Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,634,581	A→G	Y244H (TAC→CAC)	rob ←	right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,634,581	0	A	G	100.0%	191.6 / NA	54	Y244H (TAC→CAC)	rob	right oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/30); total (24/30)

GGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGG  >  NC_000913/4634449‑4634715

ggATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGt                                                                                                                                  <  2:967778/139‑1 (MQ=255)

  aTCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCa                                                                                                                                >  1:691596/1‑139 (MQ=255)

            gcagTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCNGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCTGGATAAACTc                                                                                                                      >  1:881481/1‑139 (MQ=255)

               gTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTg                                                                                                                   >  1:200961/1‑139 (MQ=255)

                 tCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCa                                                                                                                 >  2:886104/1‑139 (MQ=255)

                      gCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCg                                                                                                            <  1:1019049/139‑1 (MQ=255)

                      gCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGACGCGTCAGGTTGAGCCTTGGCATGCAGGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCg                                                                                                            <  1:671569/139‑1 (MQ=255)

                           gATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTTAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTcc                                                                                                       <  2:691596/139‑1 (MQ=255)

                           gATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTcc                                                                                                       <  1:622300/139‑1 (MQ=255)

                             ttAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCa                                                                                                     <  1:235681/139‑1 (MQ=255)

                               aaTTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGa                                                                                                   <  1:1002877/139‑1 (MQ=255)

                               aaTTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGa                                                                                                   <  2:469463/139‑1 (MQ=255)

                                 ttGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGAcc                                                                                                 <  1:353025/139‑1 (MQ=255)

                                    ggCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTc                                                                                              >  2:401715/1‑139 (MQ=255)

                                      cGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCAt                                                                                            >  1:196751/1‑139 (MQ=255)

                                           tcCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTa                                                                                       <  1:401715/139‑1 (MQ=255)

                                                   tGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATcac                                                                               >  2:459989/1‑139 (MQ=255)

                                                   tGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATcac                                                                               >  1:888043/1‑139 (MQ=255)

                                                     gCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACat                                                                             >  1:179947/1‑139 (MQ=255)

                                                             tGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTcgccg                                                                     <  1:639845/139‑1 (MQ=255)

                                                                  ggTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTg                                                                >  1:738326/1‑139 (MQ=255)

                                                                    tagtaTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGt                                                                                        >  2:742774/1‑113 (MQ=255)

                                                                    tagtaTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGt                                                                                        <  1:742774/113‑1 (MQ=255)

                                                                    tagtaTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCt                                                                 >  2:885477/1‑136 (MQ=255)

                                                                    tagtaTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCt                                                                 <  1:885477/136‑1 (MQ=255)

                                                                    tagtaTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTgca                                                              >  2:332120/1‑139 (MQ=255)

                                                                    tagtaTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTgca                                                              >  2:255685/1‑139 (MQ=255)

                                                                       taTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTgcagca                                                           >  2:291354/1‑139 (MQ=255)

                                                                            cTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCAcc                                                      >  2:1029126/1‑139 (MQ=255)

                                                                            cTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCAcc                                                      >  1:475869/1‑139 (MQ=255)

                                                                                    ccTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGccc                                              >  1:497808/1‑139 (MQ=255)

                                                                                      tGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCATATATTCGCCGCCCTGCAGCATCACCGGATGCCCCg                                            >  1:638164/1‑139 (MQ=255)

                                                                                       gACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGt                                           <  2:888043/139‑1 (MQ=255)

                                                                                             tACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAAc                                                                                    >  1:69240/1‑92 (MQ=255)

                                                                                             tACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAAc                                                                                    <  2:69240/92‑1 (MQ=255)

                                                                                              aCGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACa                                    <  1:459989/139‑1 (MQ=255)

                                                                                                          gTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGcc                        <  2:391517/139‑1 (MQ=255)

                                                                                                          gTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGcc                        <  2:678947/139‑1 (MQ=255)

                                                                                                               gCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGc                                                >  2:228433/1‑110 (MQ=255)

                                                                                                               gCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGc                                                <  1:228433/110‑1 (MQ=255)

                                                                                                                    ggCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTggg              >  1:313346/1‑139 (MQ=255)

                                                                                                                    ggCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTggg              >  1:689793/1‑139 (MQ=255)

                                                                                                                     gCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGc             <  1:603278/139‑1 (MQ=255)

                                                                                                                        tGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTaa          <  2:689793/139‑1 (MQ=255)

                                                                                                                           aCGTTACGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCACAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAAcgc       <  2:78571/139‑1 (MQ=255)

                                                                                                                            cGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAAcgcg      <  2:738326/139‑1 (MQ=255)

                                                                                                                             gTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCgg     <  2:638164/139‑1 (MQ=255)

                                                                                                                             gTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCgg     <  2:200961/139‑1 (MQ=255)

                                                                                                                             gTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCgg     <  1:426206/139‑1 (MQ=255)

                                                                                                                             gTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCgg     <  1:925019/139‑1 (MQ=255)

                                                                                                                               tCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCATACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCggtg   <  2:881481/139‑1 (MQ=255)

                                                                                                                               tCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACAt                                   >  2:831716/1‑107 (MQ=255)

                                                                                                                               tCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACAt                                   <  1:831716/107‑1 (MQ=255)

                                                                                                                                ccGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCggtgg  <  1:403389/139‑1 (MQ=255)

GGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGG  >  NC_000913/4634449‑4634715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A