Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 765 53.2 2691310 93.4% 2513683 103.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 684,616 T→C I214V (ATT→GTT)  gltL ← glutamate/aspartate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913684,6160TC100.0% 217.3 / NA 60I214V (ATT→GTT) gltLglutamate/aspartate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/34);  total (26/34)

AAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCG  >  NC_000913/684480‑684737
                                                                                                                                        |                                                                                                                         
aaaCGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAActt                                                                                                                         >  2:883612/1‑139 (MQ=255)
    gATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTAcc                                                                                                                     <  2:963603/139‑1 (MQ=255)
              gagaTGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATa                                                                                                           >  1:773787/1‑139 (MQ=255)
                              gggTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCg                                                                                           >  2:577237/1‑139 (MQ=255)
                                gTATGCGCGCCAGAGTGATTAATGCAGGCTTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGcc                                                                                         <  2:669493/139‑1 (MQ=255)
                                    gcgcgcCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTc                                                                                                                  <  2:502064/110‑1 (MQ=255)
                                    gcgcgcCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTc                                                                                                                  >  1:502064/1‑110 (MQ=255)
                                          cAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACggg                                                                               <  1:1086525/139‑1 (MQ=255)
                                           agagTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGc                                                                              <  1:124196/139‑1 (MQ=255)
                                            gagTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCa                                                                             <  1:883612/139‑1 (MQ=255)
                                            gagTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCa                                                                             <  2:1189474/139‑1 (MQ=255)
                                             cgTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTa                                                                                   >  1:109935/2‑132 (MQ=255)
                                             agTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGACTTCGACAACTTTACCCTCGTCCATAACGATCACCCGATTCGCCACTTTa                                                                                   <  2:109935/132‑1 (MQ=255)
                                                 aTTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGcc                                                                                         >  1:102548/1‑122 (MQ=255)
                                                 aTTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGcc                                                                                         <  2:102548/122‑1 (MQ=255)
                                                    aaTGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTAcc                                                                                                                     >  2:242283/1‑91 (MQ=255)
                                                    aaTGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTAcc                                                                                                                     <  1:242283/91‑1 (MQ=255)
                                                       gCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTc                                                                  >  1:635616/1‑139 (MQ=255)
                                                        cAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGccc                                                                       >  1:1133762/1‑133 (MQ=255)
                                                        cAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGccc                                                                       <  2:1133762/133‑1 (MQ=255)
                                                           gATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCg                                                                                                                 >  2:376967/1‑88 (MQ=255)
                                                           gATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCg                                                                                                                 <  1:376967/88‑1 (MQ=255)
                                                           gATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTAc                                                                                  >  1:435679/1‑119 (MQ=255)
                                                           gATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTAc                                                                                  <  2:435679/119‑1 (MQ=255)
                                                           gATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTgg                                                              >  1:1159875/1‑139 (MQ=255)
                                                                tCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcac                                                         <  2:773787/139‑1 (MQ=255)
                                                                tCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcac                                                         <  1:1095165/139‑1 (MQ=255)
                                                                  gcgAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcacca                                                       <  2:1159875/139‑1 (MQ=255)
                                                                     aGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCt                                                                                                                   >  2:1217417/1‑76 (MQ=255)
                                                                     aGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCt                                                                                                                   <  1:1217417/76‑1 (MQ=255)
                                                                     aGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGt                                                            >  2:445429/1‑131 (MQ=255)
                                                                     aGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGt                                                            <  2:932257/131‑1 (MQ=255)
                                                                     aGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGt                                                            <  1:445429/131‑1 (MQ=255)
                                                                     aGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGt                                                            >  1:932257/1‑131 (MQ=255)
                                                                          gTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATg                                               <  2:1309114/139‑1 (MQ=255)
                                                                                    cgGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTAcc                                                                                                                     <  1:353189/59‑1 (MQ=255)
                                                                                    cgGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTAcc                                                                                                                     >  2:353189/1‑59 (MQ=255)
                                                                                       tCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGCGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACcatc                                                  <  2:287334/123‑1 (MQ=255)
                                                                                       tCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACcatc                                                  >  1:287334/1‑123 (MQ=255)
                                                                                           aTTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGc                              <  2:635616/139‑1 (MQ=255)
                                                                                                 gATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGtt                         >  2:1281440/1‑138 (MQ=255)
                                                                                                 gATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGtt                         <  1:1281440/138‑1 (MQ=255)
                                                                                                      tcGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcac                                                         <  2:520958/101‑1 (MQ=255)
                                                                                                      tcGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcac                                                         >  1:520958/1‑101 (MQ=255)
                                                                                                           gaaAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACg              <  1:577237/139‑1 (MQ=255)
                                                                                                               gCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTcca                      >  2:422830/1‑127 (MQ=255)
                                                                                                               gCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTcca                      <  1:422830/127‑1 (MQ=255)
                                                                                                               gCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGT‑GGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAg         >  1:1152468/1‑139 (MQ=255)
                                                                                                                cGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGt                                                            >  2:466453/1‑88 (MQ=255)
                                                                                                                cGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGt                                                            <  1:466453/88‑1 (MQ=255)
                                                                                                                 gTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTcca                      >  2:52100/1‑125 (MQ=255)
                                                                                                                 gTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTcca                      <  1:52100/125‑1 (MQ=255)
                                                                                                                 gTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGT‑GGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTa       <  2:1152468/139‑1 (MQ=255)
                                                                                                                  tCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTa       >  1:418191/1‑139 (MQ=255)
                                                                                                                   cTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTAc      <  2:418191/139‑1 (MQ=255)
                                                                                                                            aGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcac                                                         >  1:952146/1‑79 (MQ=255)
                                                                                                                            aGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCg  <  2:579702/134‑1 (MQ=255)
                                                                                                                            aGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCg  <  2:641197/134‑1 (MQ=255)
                                                                                                                            aGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCg  >  1:579702/1‑134 (MQ=255)
                                                                                                                            aGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCg  >  1:641197/1‑134 (MQ=255)
                                                                                                                            aGTCTTCGACAACTTTACCCTCGTCCATAAAGATAACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcac                                                         <  2:952146/79‑1 (MQ=255)
                                                                                                                                        |                                                                                                                         
AAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCG  >  NC_000913/684480‑684737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A