Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
771 |
44.8 |
2153074 |
94.1% |
2026042 |
109.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
684,616 |
T→C |
I214V (ATT→GTT) |
gltL ← |
glutamate/aspartate ABC transporter ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 684,616 | 0 | T | C | 100.0%
| 72.0
/ NA
| 23 | I214V (ATT→GTT) | gltL | glutamate/aspartate ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/10); total (13/10) |
GATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATC > NC_000913/684484‑684746
|
gATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTAcc < 2:115800/139‑1 (MQ=255)
gcgcCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGATAACTTTACCCTc < 1:1033086/108‑1 (MQ=255)
gcgcCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGATAACTTTACCCTc > 2:1033086/1‑108 (MQ=255)
ccAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTc > 2:723070/1‑105 (MQ=255)
ccAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTc < 1:723070/105‑1 (MQ=255)
gATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTgg > 1:743747/1‑139 (MQ=255)
ttttCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGt < 1:26460/139‑1 (MQ=255)
gcgAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcacca > 1:415375/1‑139 (MQ=255)
gATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCttcgttcg < 2:866079/139‑1 (MQ=255)
tCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAg < 2:803417/139‑1 (MQ=255)
gATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTc > 2:546004/1‑139 (MQ=255)
atcGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTccacc > 2:182039/1‑139 (MQ=255)
aGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTcc > 1:949790/1‑139 (MQ=255)
cGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcac < 1:471707/91‑1 (MQ=255)
cGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcac > 2:471707/1‑91 (MQ=255)
cGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAg > 1:741337/1‑139 (MQ=255)
cGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAg > 1:406638/1‑139 (MQ=255)
tCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTc > 2:685397/1‑139 (MQ=255)
aGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGat < 2:1066024/139‑1 (MQ=255)
gTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGatc < 2:741337/139‑1 (MQ=255)
gTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGatc < 1:546004/139‑1 (MQ=255)
gTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGatc > 2:948959/1‑139 (MQ=255)
ttCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCCCTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGatcatc > 2:316544/1‑139 (MQ=255)
|
GATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATC > NC_000913/684484‑684746
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
