Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	690,550	A→G	V111A (GTG→GCG)	lnt ←	apolipoprotein N‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	690,550	0	A	G	100.0%	133.3 / NA	39	V111A (GTG→GCG)	lnt	apolipoprotein N‑acyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/21); total (18/21)

TATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCACGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCG  >  NC_000913/690422‑690672

tataGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCaaa                                                                                                                  <  1:30907/139‑1 (MQ=255)

  taGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                <  1:439859/139‑1 (MQ=255)

        aaCTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGCAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCaaa                                                                                                                  <  2:810576/131‑1 (MQ=255)

        aaCTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCaaa                                                                                                                  >  1:810576/1‑131 (MQ=255)

                     ggAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                <  2:43111/120‑1 (MQ=255)

                     ggAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                >  1:43111/1‑120 (MQ=255)

                       aaGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAAt                                                                                           >  1:1034716/1‑139 (MQ=255)

                              tCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACa                                                                                                    >  1:1071070/1‑123 (MQ=255)

                              tCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACa                                                                                                    <  2:1071070/123‑1 (MQ=255)

                                    cccAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCcagcag                                                                              <  2:909295/139‑1 (MQ=255)

                                     ccAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTcc                                                                                                            <  1:421109/108‑1 (MQ=255)

                                     ccAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTcc                                                                                                            >  2:421109/1‑108 (MQ=255)

                                      cAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAgcagca                                                                            <  2:1244159/139‑1 (MQ=255)

                                       aaCCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGcac                                                                           <  2:237463/139‑1 (MQ=255)

                                       aaCCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGcac                                                                           >  2:1085206/1‑139 (MQ=255)

                                            cgcAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                >  2:642375/1‑97 (MQ=255)

                                            cgcAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                <  1:642375/97‑1 (MQ=255)

                                            cgcAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                >  2:817498/1‑97 (MQ=255)

                                            cgcAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                <  1:817498/97‑1 (MQ=255)

                                            cgcAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCAccacca                                                                      >  2:64918/1‑139 (MQ=255)

                                                         tCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAAc                                                         <  2:621132/139‑1 (MQ=255)

                                                               gCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                >  2:1301464/1‑78 (MQ=255)

                                                               gCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                <  1:1301464/78‑1 (MQ=255)

                                                                 cAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                >  2:252818/1‑76 (MQ=255)

                                                                 cAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                <  1:252818/76‑1 (MQ=255)

                                                                       gCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAgcagca                                                                            >  1:1305918/1‑106 (MQ=255)

                                                                       gCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAgcagca                                                                            <  2:1305918/106‑1 (MQ=255)

                                                                                gcAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAgg                                  >  2:798559/1‑139 (MQ=255)

                                                                                gcAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAgg                                  >  1:2295/1‑139 (MQ=255)

                                                                                  aaTCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTc                                >  2:338983/1‑139 (MQ=255)

                                                                                    tCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTcgc                              <  2:2295/139‑1 (MQ=255)

                                                                                      gCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGa                            <  1:1085206/139‑1 (MQ=255)

                                                                                           gcgcAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGt                                                                                                              <  2:1265673/52‑1 (MQ=255)

                                                                                           gcgcAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGt                                                                                                              >  1:1265673/1‑52 (MQ=255)

                                                                                               aGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACAt                   >  2:456939/1‑139 (MQ=255)

                                                                                                    ggtggtTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGAcc              <  2:1034716/139‑1 (MQ=255)

                                                                                                     gtggtTTTCGGCCACAGACGCGACAGCGCGCCAGCaaa                                                                                                                  <  2:680604/38‑1 (MQ=255)

                                                                                                     gtggtTTTCGGCCACAGACGCGACAGCGCGCCAGCaaa                                                                                                                  >  1:680604/1‑38 (MQ=255)

                                                                                                                ccACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCg  <  1:338983/139‑1 (MQ=255)

TATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCACGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCG  >  NC_000913/690422‑690672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A