Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 765 53.2 2691310 93.4% 2513683 103.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,225,044 C→T *196* (TAG→TAA)  yohC ← Yip1 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,225,0440CT100.0% 221.4 / NA 69*196* (TAG→TAA) yohCYip1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/39);  total (30/39)

CACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGG  >  NC_000913/2224910‑2225170
                                                                                                                                      |                                                                                                                              
cACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGa                                                                                                                            >  2:524753/1‑139 (MQ=255)
cACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGa                                                                                                                            >  1:685673/1‑139 (MQ=255)
 aCCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGaa                                                                                                                           <  2:478245/139‑1 (MQ=255)
 aCCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGaa                                                                                                                           <  1:611657/139‑1 (MQ=255)
              ggATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGNTCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCg                                                                                                              <  1:1227329/139‑1 (MQ=255)
                    aCTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                        <  1:1040318/139‑1 (MQ=255)
                    aCTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                        <  2:685673/139‑1 (MQ=255)
                    aCTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                        <  1:501918/139‑1 (MQ=255)
                      tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                      <  1:878066/139‑1 (MQ=255)
                      tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                      <  1:765824/139‑1 (MQ=255)
                      tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                      <  1:524753/139‑1 (MQ=255)
                      tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGCCAAAGGAGAATCGTGAGGAAATGCTGCATTACTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                      <  2:917462/139‑1 (MQ=255)
                               cGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAAc                                                                                             >  1:42118/1‑139 (MQ=255)
                                    ccATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                           >  2:493428/1‑120 (MQ=255)
                                    ccATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                           >  2:167662/1‑120 (MQ=255)
                                    ccATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                           <  1:493428/120‑1 (MQ=255)
                                    ccATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                           <  1:167662/120‑1 (MQ=255)
                                    ccATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTa                                                                                        >  2:572547/1‑139 (MQ=255)
                                      atcatAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAgg                                                                                      >  2:265075/1‑139 (MQ=255)
                                       gtataagagacagaaaGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAggg                                                                                     >  1:198973/14‑139 (MQ=255)
                                               aGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                        >  2:950316/1‑112 (MQ=255)
                                               aGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                        <  1:950316/112‑1 (MQ=255)
                                               aGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagcagc                                                                             >  2:199089/1‑139 (MQ=255)
                                                gCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                           >  2:470710/1‑108 (MQ=255)
                                                gCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                           <  1:470710/108‑1 (MQ=255)
                                                 cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTa                                                                                                                              >  1:844065/1‑88 (MQ=255)
                                                 cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTa                                                                                                                              <  2:844065/88‑1 (MQ=255)
                                                 cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCAc                                                                           >  1:293543/1‑139 (MQ=255)
                                                 cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCAc                                                                           >  2:658708/1‑139 (MQ=255)
                                                          cGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                >  2:920316/1‑109 (MQ=255)
                                                          cGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                <  1:920316/109‑1 (MQ=255)
                                                           gATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACa                                                                                                                         >  2:374941/1‑83 (MQ=255)
                                                           gATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACa                                                                                                                         <  1:374941/83‑1 (MQ=255)
                                                             tCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAg                                                               <  1:658708/139‑1 (MQ=255)
                                                                 cAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACg                                                           <  2:293543/139‑1 (MQ=255)
                                                                  aaaGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                      >  1:850316/1‑95 (MQ=255)
                                                                  aaaGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                      <  2:850316/95‑1 (MQ=255)
                                                                         aaTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGa                                                                                                                    <  1:1011104/74‑1 (MQ=255)
                                                                         aaTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGa                                                                                                                    >  2:1011104/1‑74 (MQ=255)
                                                                              tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                      <  2:243792/83‑1 (MQ=255)
                                                                              tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCg                                              <  2:743308/139‑1 (MQ=255)
                                                                              tGAGGAAATGCTGCATTGCTGACATTTACGCAAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                      >  1:243792/1‑83 (MQ=255)
                                                                               gAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                             <  1:572547/139‑1 (MQ=255)
                                                                               gAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                             <  2:42118/139‑1 (MQ=255)
                                                                                                cTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                           <  1:859587/60‑1 (MQ=255)
                                                                                                cTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                           >  2:859587/1‑60 (MQ=255)
                                                                                                   aCATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagcagc                                                                             >  1:256808/1‑87 (MQ=255)
                                                                                                   aCATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagcagc                                                                             <  2:256808/87‑1 (MQ=255)
                                                                                                    cATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGcc                        <  1:265075/139‑1 (MQ=255)
                                                                                                     aTTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACg                                                                                                                     >  1:731920/1‑45 (MQ=255)
                                                                                                     aTTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACg                                                                                                                     <  2:731920/45‑1 (MQ=255)
                                                                                                       ttACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTc                     <  1:293467/139‑1 (MQ=255)
                                                                                                        tACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                             >  1:389375/1‑114 (MQ=255)
                                                                                                        tACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                             <  2:389375/114‑1 (MQ=255)
                                                                                                          cGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGcccc                                                                                                       <  1:607169/54‑1 (MQ=255)
                                                                                                          cGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGcccc                                                                                                       >  2:607169/1‑54 (MQ=255)
                                                                                                               gCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGTGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgt             >  1:77946/1‑139 (MQ=255)
                                                                                                                 aaTGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgttg           <  1:1124300/139‑1 (MQ=255)
                                                                                                                  aTGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGt                                                                                          >  2:1250962/1‑59 (MQ=255)
                                                                                                                  aTGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGt                                                                                          <  1:1250962/59‑1 (MQ=255)
                                                                                                                        cGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGGAGCTAAGGc                         >  2:864552/1‑118 (MQ=255)
                                                                                                                        cGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGc                         <  1:864552/118‑1 (MQ=255)
                                                                                                                         gTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGaa                                         <  1:1204485/101‑1 (MQ=255)
                                                                                                                         gTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGaa                                         >  2:1204485/1‑101 (MQ=255)
                                                                                                                          tCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                      >  2:204975/1‑39 (MQ=255)
                                                                                                                          tCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                      <  1:204975/39‑1 (MQ=255)
                                                                                                                          tCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCAGGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAgg  <  2:198973/139‑1 (MQ=255)
                                                                                                                          tCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTa                             <  1:429476/112‑1 (MQ=255)
                                                                                                                          tCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTa                             >  2:429476/1‑112 (MQ=255)
                                                                                                                                      |                                                                                                                              
CACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGG  >  NC_000913/2224910‑2225170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A