Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,225,044	C→T	196 (TAG→TAA)	yohC ←	Yip1 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,225,044	0	C	T	100.0%	109.0 / NA	36	196 (TAG→TAA)	yohC	Yip1 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/19); total (17/19)

CGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAG  >  NC_000913/2224908‑2225173

cgcACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTa                                                                                                                                 >  2:614275/1‑139 (MQ=255)

       ccgGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACaaa                                                                                                                          >  2:246069/1‑139 (MQ=255)

          gAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGa                                                                                                                       <  1:614275/139‑1 (MQ=255)

              aaGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAAc                                                                                                                   <  2:206304/139‑1 (MQ=255)

                   tttACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                              <  1:237218/139‑1 (MQ=255)

                        tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                         <  1:188085/139‑1 (MQ=255)

                        tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                         <  1:86358/139‑1 (MQ=255)

                            gCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGt                                                                                                     >  1:209639/1‑139 (MQ=255)

                                 cGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAAc                                                                                                >  2:71357/1‑139 (MQ=255)

                                      ccATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGCGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTa                                                                                           >  1:309925/1‑139 (MQ=255)

                                      ccATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTa                                                                                           >  1:44564/1‑139 (MQ=255)

                                                 aGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCACCATAGTATAACGGTTAGGGCGagcagc                                                                                >  1:461870/1‑139 (MQ=255)

                                                     aaaaGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACtt                                                                            >  1:676167/1‑139 (MQ=255)

                                                        aGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCa                                                                         >  1:116570/1‑139 (MQ=255)

                                                                           aaTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                           <  1:335227/86‑1 (MQ=255)

                                                                           aaTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                           >  2:335227/1‑86 (MQ=255)

                                                                             tCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                              <  1:467393/81‑1 (MQ=255)

                                                                             tCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                              >  2:467393/1‑81 (MQ=255)

                                                                                 gAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                                <  1:755773/139‑1 (MQ=255)

                                                                                 gAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCACCACGCCAATCGCCAGCGt                                                <  2:461870/139‑1 (MQ=255)

                                                                                     aaaTGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGaa                                            <  2:116570/139‑1 (MQ=255)

                                                                                     aaaTGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGaa                                            <  2:309925/139‑1 (MQ=255)

                                                                                           tgcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTagag                                      <  2:376132/139‑1 (MQ=255)

                                                                                                  cTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAAc                                                                                                                             >  1:553188/1‑45 (MQ=255)

                                                                                                  cTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAAc                                                                                                                             <  2:553188/45‑1 (MQ=255)

                                                                                                        tttACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCtt                         <  1:246069/139‑1 (MQ=255)

                                                                                                          tACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTcc                       >  1:426186/1‑139 (MQ=255)

                                                                                                            cGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                                >  1:175329/1‑112 (MQ=255)

                                                                                                            cGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                                <  2:175329/112‑1 (MQ=255)

                                                                                                            cGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGG‑‑AGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCtt                   >  1:10040/1‑139 (MQ=255)

                                                                                                                 gCAATGCAACGTCAAAGAACTTTTTAGCACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                                >  1:498660/1‑107 (MQ=255)

                                                                                                                 gCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGTCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                                <  2:498660/107‑1 (MQ=255)

                                                                                                                            tCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAgg     <  2:44564/139‑1 (MQ=255)

                                                                                                                            tCAAAGAACTCTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGTCGAGCAGCACTTCCAGTACCAGCACGCCAATCGACAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAgg     >  2:105938/1‑139 (MQ=255)

                                                                                                                              aaaGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGaa   <  2:199153/139‑1 (MQ=255)

                                                                                                                              aaaGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTA‑CAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAg  <  1:206601/139‑1 (MQ=255)

CGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAG  >  NC_000913/2224908‑2225173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A