Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 773 79.9 4112934 92.2% 3792125 104.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,225,044 C→T *196* (TAG→TAA)  yohC ← Yip1 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,225,0440CT100.0% 202.7 / NA 63*196* (TAG→TAA) yohCYip1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/35);  total (28/35)

CGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAG  >  NC_000913/2224908‑2225173
                                                                                                                                        |                                                                                                                                 
cgcACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATTCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTa                                                                                                                                 >  1:1060207/1‑139 (MQ=255)
   aCCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGaa                                                                                                                              <  1:2014609/139‑1 (MQ=255)
       ccgGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGGGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACaaa                                                                                                                          >  2:604910/1‑139 (MQ=255)
           aGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGAt                                                                                                                      <  1:833727/139‑1 (MQ=255)
                ggATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCg                                                                                                                 <  1:663806/139‑1 (MQ=255)
                   tttACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATTCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                              <  2:1060207/139‑1 (MQ=255)
                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGGGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGcc                                                                                                            <  1:604910/139‑1 (MQ=255)
                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGAt                                                                                                                      <  1:840521/129‑1 (MQ=255)
                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGAt                                                                                                                      >  2:840521/1‑129 (MQ=255)
                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGcc                                                                                                            >  2:1089440/1‑139 (MQ=255)
                        tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCTATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                         <  1:1273998/139‑1 (MQ=255)
                        tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                         <  2:765341/139‑1 (MQ=255)
                           ggCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAg                                                                                                      >  2:1379991/1‑139 (MQ=255)
                              tAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                   <  2:598318/139‑1 (MQ=255)
                              tAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                   <  1:1175519/139‑1 (MQ=255)
                              tAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                   <  2:1774084/139‑1 (MQ=255)
                                          catAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACaa                                                                                                                           <  1:1492388/103‑1 (MQ=255)
                                          catAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACaa                                                                                                                           >  2:1492388/1‑103 (MQ=255)
                                               cGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagca                                                                                  >  1:544270/1‑139 (MQ=255)
                                                gAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagcag                                                                                 <  2:565202/139‑1 (MQ=255)
                                                  gCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAgcagca                                                                               <  1:1379991/139‑1 (MQ=255)
                                                   cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGt                                                                                                                <  1:1858035/105‑1 (MQ=255)
                                                   cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGt                                                                                                                >  2:1858035/1‑105 (MQ=255)
                                                   cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                         <  1:177463/112‑1 (MQ=255)
                                                   cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                         >  2:177463/1‑112 (MQ=255)
                                                   cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagcagc                                                                                >  1:933336/1‑137 (MQ=255)
                                                   cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagcagc                                                                                <  2:933336/137‑1 (MQ=255)
                                                   cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCAc                                                                              >  2:353173/1‑139 (MQ=255)
                                                   cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCAc                                                                              >  2:1720858/1‑139 (MQ=255)
                                                        aGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGATCTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCa                                                                         >  1:1097091/1‑139 (MQ=255)
                                                          tGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCATCAATGCAACGTCAAAGATCTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGt                                                                       <  2:1097091/139‑1 (MQ=255)
                                                            cGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGcc                                                                                                            <  1:1412256/100‑1 (MQ=255)
                                                            cGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGcc                                                                                                            >  2:1412256/1‑100 (MQ=255)
                                                                cGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagcagc                                                                                >  1:1111986/1‑124 (MQ=255)
                                                                cGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagcagc                                                                                <  2:1111986/124‑1 (MQ=255)
                                                                 gTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCa                                                                <  1:353173/139‑1 (MQ=255)
                                                                   cAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACg                                                              <  1:1720858/139‑1 (MQ=255)
                                                                            aTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAg                                                                  >  2:150932/1‑126 (MQ=255)
                                                                            aTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAg                                                                  <  1:150932/126‑1 (MQ=255)
                                                                             tCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                           >  1:238953/1‑84 (MQ=255)
                                                                             tCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                           >  1:1219201/1‑84 (MQ=255)
                                                                             tCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                           <  2:1219201/84‑1 (MQ=255)
                                                                             tCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                           <  2:238953/84‑1 (MQ=255)
                                                                                  aGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTc                                               >  2:91620/1‑139 (MQ=255)
                                                                                      aaTGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAAc                                           <  2:544270/139‑1 (MQ=255)
                                                                                       aTGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCg                                                                                      >  2:29968/1‑95 (MQ=255)
                                                                                       aTGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCg                                                                                      <  1:29968/95‑1 (MQ=255)
                                                                                         gctgcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTag                                        >  2:917945/1‑139 (MQ=255)
                                                                                               ttGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGc                                  >  2:194196/1‑139 (MQ=255)
                                                                                                  cTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTaa                               >  1:5542/1‑139 (MQ=255)
                                                                                                      cATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGcc                           <  1:917945/139‑1 (MQ=255)
                                                                                                             gCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCctct                    <  1:91620/139‑1 (MQ=255)
                                                                                                                 gCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgt                >  2:1523819/1‑139 (MQ=255)
                                                                                                                 gCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgt                <  2:829581/139‑1 (MQ=255)
                                                                                                                  cAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGcc                           >  1:1238361/1‑127 (MQ=255)
                                                                                                                  cAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAATATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGcc                           <  2:1238361/127‑1 (MQ=255)
                                                                                                                            tCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAgg     <  2:5542/139‑1 (MQ=255)
                                                                                                                              aaaGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGaa   <  1:194196/139‑1 (MQ=255)
                                                                                                                               aaGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTagag                                      >  2:666116/1‑103 (MQ=255)
                                                                                                                               aaGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTagag                                      <  1:666116/103‑1 (MQ=255)
                                                                                                                               aaGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAg  >  2:1712392/1‑139 (MQ=255)
                                                                                                                                aGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagca                                                                                  >  1:1526180/1‑58 (MQ=255)
                                                                                                                                aGAACTTTTTAAAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagca                                                                                  <  2:1526180/58‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
CGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAG  >  NC_000913/2224908‑2225173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A