Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,548,360	C→T	D320D (GAC→GAT)	fdnG →	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,548,360	0	C	T	100.0%	155.7 / NA	49	D320D (GAC→GAT)	fdnG	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/23); total (26/23)

TCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGC  >  NC_000913/1548225‑1548490

tCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGt                                                                                                                                 <  1:900313/139‑1 (MQ=255)

              aCATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTa                                                                                                                   <  1:1310573/139‑1 (MQ=255)

                 ttACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTacga                                                                                                                <  2:343404/139‑1 (MQ=255)

                        ccTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAAACAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCa                                                                                                                          >  1:1093768/1‑122 (MQ=255)

                        ccTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAAACAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCa                                                                                                                          <  2:1093768/122‑1 (MQ=255)

                         cTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGaaa                                                                                                        <  1:88951/139‑1 (MQ=255)

                                           tACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAAt                                                                                      <  1:1359557/139‑1 (MQ=255)

                                             ccTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCg                                                                                    >  1:1036373/1‑139 (MQ=255)

                                                       aacaacAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACt                                                                          >  1:1514081/1‑139 (MQ=255)

                                                           acaaAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGaaa                                                                                                        <  2:696549/105‑1 (MQ=255)

                                                           acaaAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGaaa                                                                                                        >  1:696549/1‑105 (MQ=255)

                                                               aaTCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTc                                                                  >  1:392895/1‑139 (MQ=255)

                                                                       ccGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGaaaa                                                          <  1:1236097/139‑1 (MQ=255)

                                                                          aaTACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACgg                                                       >  2:358066/1‑139 (MQ=255)

                                                                                ttAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTgg                                                                              >  1:1745195/1‑110 (MQ=255)

                                                                                ttAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTgg                                                                              <  2:1745195/110‑1 (MQ=255)

                                                                                        tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTc                                                                                  >  2:929861/1‑98 (MQ=255)

                                                                                        tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTc                                                                                  <  1:929861/98‑1 (MQ=255)

                                                                                        tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAAcgcg                                         >  1:1418544/1‑139 (MQ=255)

                                                                                        tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAAcgcg                                         >  2:536948/1‑139 (MQ=255)

                                                                                        tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAAcgcg                                         >  1:537776/1‑139 (MQ=255)

                                                                                        tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAAcg                                           <  1:68878/137‑1 (MQ=255)

                                                                                        tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAAcg                                           >  2:68878/1‑137 (MQ=255)

                                                                                         acacCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGa                                        >  2:703014/1‑139 (MQ=255)

                                                                                         acacCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGa                                        >  2:626469/1‑139 (MQ=255)

                                                                                         acacCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGa                                        >  2:597495/1‑139 (MQ=255)

                                                                                         acacCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCCAAGATGGTCTGTTCAGCGGCTACGACGATGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCACCTCGATGAAAACGGCTATGCGAAACGCGa                                        >  2:1792132/1‑139 (MQ=255)

                                                                                          cacCAACACCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGAt                                       >  2:1642869/1‑139 (MQ=255)

                                                                                             cAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGaaaa                                                          <  2:1158883/117‑1 (MQ=255)

                                                                                             cAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGaaaa                                                          >  1:1158883/1‑117 (MQ=255)

                                                                                                  ccAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACt                               >  2:1388841/1‑139 (MQ=255)

                                                                                                     gCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGAc                            >  2:864538/1‑139 (MQ=255)

                                                                                                      ccTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACt                           <  1:1642869/139‑1 (MQ=255)

                                                                                                          ctgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGaaaa                                                          <  2:615527/104‑1 (MQ=255)

                                                                                                          ctgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGaaaa                                                          >  1:615527/1‑104 (MQ=255)

                                                                                                             gTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgc                    <  1:358066/139‑1 (MQ=255)

                                                                                                              tGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg                   <  1:212923/139‑1 (MQ=255)

                                                                                                                cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg                   <  2:871952/137‑1 (MQ=255)

                                                                                                                cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg                   >  1:871952/1‑137 (MQ=255)

                                                                                                                cGTGATGATTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCt                 <  2:1036373/139‑1 (MQ=255)

                                                                                                                  tgatgaTTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGaaa                                                                                                        <  2:180625/50‑1 (MQ=255)

                                                                                                                  tgatgaTTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGaaa                                                                                                        >  1:180625/1‑50 (MQ=255)

                                                                                                                    atgatTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgc                    <  2:1268841/132‑1 (MQ=255)

                                                                                                                    atgatTTTGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTAAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgc                    >  1:1268841/1‑132 (MQ=255)

                                                                                                                          ttGCTTTCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGaa       <  1:864538/139‑1 (MQ=255)

                                                                                                                               ttCGAAGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACctgc  <  1:626469/139‑1 (MQ=255)

                                                                                                                                tCGAAGATGGTCTGTTCAGCGGCTACGACGCTGaaa                                                                                                        <  2:1518064/36‑1 (MQ=255)

                                                                                                                                tCGAAGATGGTCTGTTCAGCGGCTACGACGCTGaaa                                                                                                        >  1:1518064/1‑36 (MQ=255)

                                                                                                                                    aGATGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg                   >  1:452974/1‑117 (MQ=255)

TCGTTCCGGTACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGC  >  NC_000913/1548225‑1548490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A