Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
777 |
59.2 |
2955938 |
92.5% |
2734242 |
106.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,548,360 |
C→T |
D320D (GAC→GAT) |
fdnG → |
formate dehydrogenase‑N, alpha subunit, nitrate‑inducible |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,548,360 | 0 | C | T | 100.0%
| 151.6
/ NA
| 47 | D320D (GAC→GAT) | fdnG | formate dehydrogenase‑N, alpha subunit, nitrate‑inducible |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (25/22); total (25/22) |
TACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCT > NC_000913/1548234‑1548475
|
tACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATgg > 1:1289114/1‑129 (MQ=255)
tACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATgg < 2:1289114/129‑1 (MQ=255)
aCATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTa > 1:1000915/1‑139 (MQ=255)
ttACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTacga > 2:1261607/1‑139 (MQ=255)
tACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTacgac > 2:28616/1‑139 (MQ=255)
aCGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTacgacg < 2:1458410/139‑1 (MQ=255)
ttCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCTCAGCGGCTacgacg > 2:476103/1‑138 (MQ=255)
ttCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGTTCTCAGCGGCTacgacg < 1:476103/138‑1 (MQ=255)
tGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGaaaa > 2:717813/1‑139 (MQ=255)
gCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCa > 1:297647/1‑139 (MQ=255)
cGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCaa < 1:717813/139‑1 (MQ=255)
tGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGa > 2:318302/1‑139 (MQ=255)
ccTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCg < 1:318302/139‑1 (MQ=255)
aaaaCAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGaa < 1:28616/139‑1 (MQ=255)
aacaacAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACt > 1:219569/1‑139 (MQ=255)
caaAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAg < 1:310995/87‑1 (MQ=255)
caaAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAg > 2:310995/1‑87 (MQ=255)
gCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAg < 2:82391/77‑1 (MQ=255)
gCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAg > 1:82391/1‑77 (MQ=255)
gCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGaaa > 1:36479/1‑139 (MQ=255)
ttACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTc < 1:145020/99‑1 (MQ=255)
ttACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTc > 2:145020/1‑99 (MQ=255)
tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAAc > 2:1269061/1‑79 (MQ=255)
tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAAc < 1:1269061/79‑1 (MQ=255)
tACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAAcgcg > 2:867113/1‑139 (MQ=255)
aCGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTa < 2:1170613/58‑1 (MQ=255)
aCGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTa > 1:1170613/1‑58 (MQ=255)
aCGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAac > 2:1104939/1‑139 (MQ=255)
aCGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAac < 2:297647/139‑1 (MQ=255)
cGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAcaca < 2:1000915/139‑1 (MQ=255)
ccAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACt > 1:1456734/1‑139 (MQ=255)
gcttgctgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGCCCTgg < 2:1354818/86‑1 (MQ=255)
gCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTgg > 1:1354818/1‑89 (MQ=255)
ccTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTgg > 2:97336/1‑88 (MQ=255)
ccTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTgg < 1:97336/88‑1 (MQ=255)
ccTGCTGGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACt < 2:36479/139‑1 (MQ=255)
ctgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATc > 2:758187/1‑139 (MQ=255)
ctgATGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATc > 2:641213/1‑139 (MQ=255)
tgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTa > 2:749731/1‑46 (MQ=255)
tgGTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTa < 1:749731/46‑1 (MQ=255)
gTGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAACCACTGACTCATCcgc > 2:1392198/1‑139 (MQ=255)
tGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAACCACTGACTCATCcgcg < 1:1392198/139‑1 (MQ=255)
tGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg < 2:219569/139‑1 (MQ=255)
tGCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCcgcg < 2:1220044/139‑1 (MQ=255)
gCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAATGGCTATGCGAAACGCGATGAAACACTGACt > 2:493307/1‑130 (MQ=255)
gCGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAATGGCTATGCGAAACGCGATGAAACACTGACt < 1:493307/130‑1 (MQ=255)
cGTGATGATTTTGCTTTCGAAGATGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCt < 2:1456734/139‑1 (MQ=255)
|
TACGGACATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAATACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGT‑‑TCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCT > NC_000913/1548234‑1548475
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
