Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
764 |
42.0 |
2018750 |
95.5% |
1927906 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
450,764 |
C→T |
A283T (GCT→ACT) |
cyoA ← |
cytochrome o ubiquinol oxidase subunit II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 450,764 | 0 | C | T | 100.0%
| 119.5
/ NA
| 39 | A283T (GCT→ACT) | cyoA | cytochrome o ubiquinol oxidase subunit II |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/20); total (19/20) |
TTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTG > NC_000913/450630‑450893
|
ttttCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGTTCACCTTCTGGCTGGCTCATGTCCATGCTCTTACCGTGAGTCATa < 1:582886/139‑1 (MQ=255)
tCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAAt < 2:683709/139‑1 (MQ=255)
ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAAc < 1:576334/120‑1 (MQ=255)
ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAAc > 2:576334/1‑120 (MQ=255)
ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCaa > 1:818109/1‑139 (MQ=255)
ggCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAAc > 2:762288/1‑103 (MQ=255)
ggCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAAc < 1:762288/103‑1 (MQ=255)
ggCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACg < 2:818109/139‑1 (MQ=255)
gccgcaTTCCCCGTGGCTCATGTCCATGCCTTCCATACCGTCGTGTGCTCTGTACTCACATTCTGGCTGGGTCTTGTCTAGGCTCTTTCCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACg < 1:637601/134‑1 (MQ=255)
cGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACg < 2:74589/137‑1 (MQ=255)
cGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACg > 1:74589/1‑137 (MQ=255)
ttCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGgag < 2:223627/139‑1 (MQ=255)
gCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTcc > 1:975039/1‑139 (MQ=255)
tCATGTCCATTCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAc < 2:511073/139‑1 (MQ=255)
gTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg < 2:586649/139‑1 (MQ=255)
cGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGc > 2:376155/1‑139 (MQ=255)
cGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGc > 1:426979/1‑139 (MQ=255)
gtgtGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGcc > 1:851934/1‑139 (MQ=255)
tgtgCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGttt > 1:988043/1‑92 (MQ=255)
tgtgCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGttt < 2:988043/92‑1 (MQ=255)
gtgCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACt < 1:969142/53‑1 (MQ=255)
gtgCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACt > 2:969142/1‑53 (MQ=255)
gCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGaa < 2:851934/139‑1 (MQ=255)
cTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAAc < 1:663698/139‑1 (MQ=255)
ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCaaa < 1:935242/61‑1 (MQ=255)
ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCaaa > 2:935242/1‑61 (MQ=255)
ggTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGCTAATTCCATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTAGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGAc > 2:675396/1‑139 (MQ=255)
aTGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATgg < 2:975039/139‑1 (MQ=255)
aTGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCg > 2:877390/1‑139 (MQ=255)
gCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAc > 2:1007001/1‑75 (MQ=255)
gCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAc < 1:1007001/75‑1 (MQ=255)
tACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGc < 1:497472/112‑1 (MQ=255)
tACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGc > 2:745088/1‑112 (MQ=255)
tACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGc < 1:745088/112‑1 (MQ=255)
tACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGc > 2:497472/1‑112 (MQ=255)
tACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTg > 2:762228/1‑139 (MQ=255)
tACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTg > 2:938460/1‑139 (MQ=255)
aCCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg > 1:683208/1‑73 (MQ=255)
aCCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg < 2:683208/73‑1 (MQ=255)
|
TTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTG > NC_000913/450630‑450893
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
