Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	450,764	C→T	A283T (GCT→ACT)	cyoA ←	cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	450,764	0	C	T	100.0%	150.6 / NA	47	A283T (GCT→ACT)	cyoA	cytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/20); total (27/20)

CGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGC  >  NC_000913/450635‑450894

cGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACtt                                                                                                                            <  1:607205/139‑1 (MQ=255)

cGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACtt                                                                                                                            <  1:568582/139‑1 (MQ=255)

         ttATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTAc                                                                                                                   >  1:1744725/1‑139 (MQ=255)

                 ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATa                                                                                                                                 <  1:1168781/117‑1 (MQ=255)

                 ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATa                                                                                                                                 >  2:1168781/1‑117 (MQ=255)

                 ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCaa                                                                                                           >  2:548443/1‑139 (MQ=255)

                 ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCaa                                                                                                           >  1:1548469/1‑139 (MQ=255)

                 ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCaa                                                                                                           >  1:1834407/1‑139 (MQ=255)

                           tttAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAAc                                                                                                                              <  2:44562/110‑1 (MQ=255)

                           tttAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAAc                                                                                                                              >  1:44562/1‑110 (MQ=255)

                                   gCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACtt                                                                                                                            >  2:67314/1‑104 (MQ=255)

                                   gCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACtt                                                                                                                            <  1:67314/104‑1 (MQ=255)

                                    cGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCa                                                                                                                                   >  2:203126/1‑96 (MQ=255)

                                      gATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTgg                                                                                      >  1:827221/1‑139 (MQ=255)

                                             cgTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTc                                                                                                    <  2:881133/118‑1 (MQ=255)

                                             cgTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTc                                                                                                    >  1:881133/1‑118 (MQ=255)

                                                          cATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTg                                                                  >  1:1453668/1‑139 (MQ=255)

                                                            tGCCTTCCATACCTTTGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACg                                                                                          <  1:1099253/113‑1 (MQ=255)

                                                            tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACtt                                                                                                                            >  1:1739662/1‑79 (MQ=255)

                                                            tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACtt                                                                                                                            <  2:1739662/79‑1 (MQ=255)

                                                            tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTcgcg                                                                                          >  2:1099253/1‑113 (MQ=255)

                                                                  ccATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCt                                                         >  1:747909/1‑139 (MQ=255)

                                                                   cATACCTTCGTGTGCACTGTGCTCACCTTCTGGCTGGATCATGTCCATGCTCTTACCGTGAGTCATAAACTTGCTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTa                                                        >  1:1226952/1‑139 (MQ=255)

                                                                             tgtgCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCa                                              >  1:1009042/1‑139 (MQ=255)

                                                                                  gcTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGtttt                                         <  1:548443/139‑1 (MQ=255)

                                                                                      tgCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTg                                                                                       >  1:1049089/1‑90 (MQ=255)

                                                                                      tgCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTg                                                                                       <  2:1049089/90‑1 (MQ=255)

                                                                                            ccTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                               <  2:59694/139‑1 (MQ=255)

                                                                                            ccTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                               <  2:1009042/139‑1 (MQ=255)

                                                                                             cTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGc                              <  2:1744725/139‑1 (MQ=255)

                                                                                                ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg                                                                     >  1:1614760/1‑98 (MQ=255)

                                                                                                ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg                                                                     <  2:1614760/98‑1 (MQ=255)

                                                                                                ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTTCGCTAGGCGCGGCCAGTTTTTc                                       >  1:1683665/1‑128 (MQ=255)

                                                                                                ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTTCGCTAGGCGCGGCCAGTTTTTc                                       <  2:1683665/128‑1 (MQ=255)

                                                                                                ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCAt                           >  2:1514979/1‑139 (MQ=255)

                                                                                                ctggctggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCAt                           >  1:207007/1‑139 (MQ=255)

                                                                                                     tggGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAg                      >  1:212098/1‑139 (MQ=255)

                                                                                                             gTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                               <  2:460099/122‑1 (MQ=255)

                                                                                                             gTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                               >  1:460099/1‑122 (MQ=255)

                                                                                                                   gCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCt                                                                                                   <  2:1740628/49‑1 (MQ=255)

                                                                                                                   gCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCt                                                                                                   >  1:1740628/1‑49 (MQ=255)

                                                                                                                   gCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGc                                 >  2:1799713/1‑114 (MQ=255)

                                                                                                                   gCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGc                                 <  1:1799713/114‑1 (MQ=255)

                                                                                                                    ctctTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCg       <  2:1834407/139‑1 (MQ=255)

                                                                                                                        tACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGt                                                                                                     <  2:1755611/42‑1 (MQ=255)

                                                                                                                        tACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGt                                                                                                     >  1:1755611/1‑42 (MQ=255)

                                                                                                                         aCCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGc  >  1:1821900/1‑139 (MQ=255)

CGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTA‑TTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGC  >  NC_000913/450635‑450894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A