Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
762 |
35.5 |
1545288 |
95.5% |
1475750 |
117.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,403,059 |
A→G |
I66T (ATT→ACT) |
abgB ← |
p‑aminobenzoyl‑glutamate hydrolase, B subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,403,059 | 0 | A | G | 100.0%
| 94.1
/ NA
| 27 | I66T (ATT→ACT) | abgB | p‑aminobenzoyl‑glutamate hydrolase, B subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/14); total (13/14) |
TGTCCGCAACCGTGACCATTTTCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAATAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATA > NC_000913/1402924‑1403193
|
tgtCCGCAACCGAGACCATTTTCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTaa < 1:127100/139‑1 (MQ=255)
tttCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAAGCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGcc > 2:292537/1‑139 (MQ=255)
aGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAg < 2:90670/139‑1 (MQ=255)
ggTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGc > 2:650848/1‑139 (MQ=255)
cgcgcAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCg < 2:83413/120‑1 (MQ=255)
cgcgcAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCg > 1:83413/1‑120 (MQ=255)
cgcgcAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCa > 1:613618/1‑139 (MQ=255)
gCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCg > 1:760013/1‑139 (MQ=255)
atatTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAAc > 1:39418/1‑139 (MQ=255)
cagGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACgt < 1:292537/139‑1 (MQ=255)
agGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACgtg > 1:364373/1‑139 (MQ=255)
ggCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTc > 1:215425/1‑111 (MQ=255)
ggCGATAACCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTc < 2:215425/111‑1 (MQ=255)
aCCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGgat < 1:391920/139‑1 (MQ=255)
aCCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGgat > 2:703954/1‑139 (MQ=255)
aCCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGgat < 2:661235/139‑1 (MQ=255)
aCCGGTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGgat > 1:661235/1‑139 (MQ=255)
ggTTTGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGgatgat > 2:298509/1‑139 (MQ=255)
tttGCCTTGACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATcc < 1:703954/139‑1 (MQ=255)
gACCAAACGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTg < 2:225561/139‑1 (MQ=255)
aCGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGaa < 2:243966/97‑1 (MQ=255)
aCGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGaa > 1:243966/1‑97 (MQ=255)
aCGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGc > 2:16600/1‑139 (MQ=255)
aCGAAGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGc < 1:350747/139‑1 (MQ=255)
aaGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAat < 1:650848/139‑1 (MQ=255)
aGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAata < 2:250926/139‑1 (MQ=255)
aGCAGTAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAata < 1:298509/139‑1 (MQ=255)
|
TGTCCGCAACCGTGACCATTTTCACCGGGCGTCACGGATGTAGGTTGCGCGCAACCTGCTTGCTGACTTAAACCTGCCAGGGCGTCATATTCTCCCAGCAGGGCGATAACCGGTTTGCCTTGACCAAACGAAGCAATAAAGGCATTTGGGATATTGCCTACGTTGCGGGTAACGGTGAAGCCTGCAGATTCCAGCGCCGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATA > NC_000913/1402924‑1403193
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
