Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,521,048	A→G	V673A (GTC→GCC)	yncD ←	putative iron outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,521,048	0	A	G	100.0%	167.0 / NA	47	V673A (GTC→GCC)	yncD	putative iron outer membrane transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/24); total (23/24)

GCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGGCGCTTTTGCC  >  NC_000913/1520912‑1521179

gCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCa                                                                                                                                   >  1:487008/1‑139 (MQ=255)

    tACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGa                                                                                                                               >  1:154319/1‑139 (MQ=255)

       aaTCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAg                                                                                                                            <  2:291196/139‑1 (MQ=255)

           gTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAAcc                                                                                                                        >  1:101497/1‑139 (MQ=255)

           gTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAAcc                                                                                                                        >  1:224334/1‑139 (MQ=255)

                                      ggCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCa                                                                                                                                   >  1:214614/1‑101 (MQ=255)

                                      ggCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCa                                                                                                                                   <  2:214614/101‑1 (MQ=255)

                                      ggCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATc                                                                                             >  2:389629/1‑139 (MQ=255)

                                              ggTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     <  1:767025/139‑1 (MQ=255)

                                                        cAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                           <  2:643200/139‑1 (MQ=255)

                                                        cAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                           >  2:602418/1‑139 (MQ=255)

                                                         aaaTCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     >  2:72557/1‑128 (MQ=255)

                                                         aaaTCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     <  1:72557/128‑1 (MQ=255)

                                                              tcCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGt                                                                     <  1:171896/139‑1 (MQ=255)

                                                                cACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                           >  2:660362/1‑131 (MQ=255)

                                                                cACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATc                                                                           <  1:660362/131‑1 (MQ=255)

                                                                cACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTa                                                                   >  2:590639/1‑139 (MQ=255)

                                                                       tatTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATtgtg                                                            <  2:101497/139‑1 (MQ=255)

                                                                       tatTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATtgtg                                                            <  2:224334/139‑1 (MQ=255)

                                                                       tatTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATtgtg                                                            >  1:311964/1‑139 (MQ=255)

                                                                             tGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                         >  1:716985/1‑120 (MQ=255)

                                                                             tGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                         <  2:716985/120‑1 (MQ=255)

                                                                             tGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAtt                                                      <  1:445032/139‑1 (MQ=255)

                                                                               ccGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     <  1:512847/106‑1 (MQ=255)

                                                                               ccGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     >  2:512847/1‑106 (MQ=255)

                                                                                   cacCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAAc                                                                                                                     <  1:220120/70‑1 (MQ=255)

                                                                                   cacCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAAc                                                                                                                     >  2:220120/1‑70 (MQ=255)

                                                                                      cATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTtata                                             <  2:311964/139‑1 (MQ=255)

                                                                                              cGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     <  1:391080/91‑1 (MQ=255)

                                                                                              cGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGAc                                                                                     >  2:391080/1‑91 (MQ=255)

                                                                                                   gggCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCACTGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAg                                <  2:487008/139‑1 (MQ=255)

                                                                                                    ggCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGaa                                                                                                                          <  1:339047/48‑1 (MQ=255)

                                                                                                    ggCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGaa                                                                                                                          >  2:339047/1‑48 (MQ=255)

                                                                                                        aaGGTTCGTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCcgac                           <  1:389629/139‑1 (MQ=255)

                                                                                                               gTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAAt                                                                                                     >  2:536741/1‑58 (MQ=255)

                                                                                                               gTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAAt                                                                                                     <  1:536741/58‑1 (MQ=255)

                                                                                                               gTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                         >  2:653056/1‑86 (MQ=255)

                                                                                                               gTAATATCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCca                                                                         <  1:653056/86‑1 (MQ=255)

                                                                                                                     tCGCCCGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGAc              >  2:754118/1‑139 (MQ=255)

                                                                                                                        cccGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGgc           >  1:10255/1‑139 (MQ=255)

                                                                                                                         ccGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCG‑ATAATTTAt                                                                                              <  2:335259/54‑1 (MQ=255)

                                                                                                                          cGTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGgcgc         >  2:720661/1‑139 (MQ=255)

                                                                                                                           gTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATTGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTg                                                     <  1:461333/94‑1 (MQ=255)

                                                                                                                           gTTTGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTg                                                     >  2:461333/1‑94 (MQ=255)

                                                                                                                             ttGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAt                                                       <  2:388688/90‑1 (MQ=255)

                                                                                                                             ttGACTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAAt                                                       >  1:388688/1‑90 (MQ=255)

                                                                                                                                 cTCATTGGCAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGGCGCTTTTGcc  <  1:720661/139‑1 (MQ=255)

GCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACACGACCAAATAAATCCACAGTTAAATTGTGGTAATTGTATTTATACCCGGTGAATAAGCCGACGAGAGTATAAGACGGCGCTTTTGCC  >  NC_000913/1520912‑1521179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A