Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,297,668	C→T	D567N (GAC→AAC)	fdhF ←	formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,297,668	0	C	T	100.0%	164.5 / NA	51	D567N (GAC→AAC)	fdhF	formate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/29); total (22/29)

TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACT  >  NC_000913/4297536‑4297787

tGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTgg                                                                                                                   <  2:722718/139‑1 (MQ=255)

       tgCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTAcc                                                                                                            >  1:618128/1‑139 (MQ=255)

              ccAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGc                                                                                                     <  1:258459/139‑1 (MQ=255)

              ccAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGc                                                                                                     <  1:276496/139‑1 (MQ=255)

                aGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAg                                                                                                   <  1:173187/139‑1 (MQ=255)

                     catcaGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaa                                                                                              >  2:762830/1‑139 (MQ=255)

                        cagccagcGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                           <  1:762830/139‑1 (MQ=255)

                             agcGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTgcg                                                                                      >  2:33653/1‑139 (MQ=255)

                                   gccAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGAc                                                                                <  1:265179/139‑1 (MQ=255)

                                               caGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTAt                                                                    >  1:125178/1‑139 (MQ=255)

                                                 gTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCa                                                                  >  2:322848/1‑139 (MQ=255)

                                                  ttACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCaa                                                                 >  2:444361/1‑139 (MQ=255)

                                                     ccGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                              <  2:93872/139‑1 (MQ=255)

                                                        gTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAg                                                                                  <  2:679343/116‑1 (MQ=255)

                                                        gTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAg                                                                                  >  1:679343/1‑116 (MQ=255)

                                                          cATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                           <  2:651157/105‑1 (MQ=255)

                                                          cATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                           >  1:651157/1‑105 (MQ=255)

                                                            tCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCttt                                                       >  1:199007/1‑139 (MQ=255)

                                                             cGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTa                                                      <  1:407684/139‑1 (MQ=255)

                                                               aaCGGCAAGAGTAGTGACCAACTTAACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTaaa                                                    <  1:429308/139‑1 (MQ=255)

                                                                    cAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGAt                                               <  1:33653/139‑1 (MQ=255)

                                                                     aaGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATa                                              >  1:309132/1‑139 (MQ=255)

                                                                         gtagtGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaagaa                                          <  1:314879/139‑1 (MQ=255)

                                                                                    cTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTa                                                      <  2:509855/116‑1 (MQ=255)

                                                                                    cTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTa                                                      >  1:509855/1‑116 (MQ=255)

                                                                                      tCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCgg                             <  1:431004/139‑1 (MQ=255)

                                                                                       cACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                            <  1:711960/139‑1 (MQ=255)

                                                                                              cGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGaa                     >  2:47930/1‑139 (MQ=255)

                                                                                              cGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGaa                     >  2:263549/1‑139 (MQ=255)

                                                                                               gTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaag                                            <  1:207031/115‑1 (MQ=255)

                                                                                               gTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaag                                            >  2:207031/1‑115 (MQ=255)

                                                                                               gTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAg                    >  1:19260/1‑139 (MQ=255)

                                                                                                        aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCGGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGAt                                               <  1:575314/103‑1 (MQ=255)

                                                                                                        aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCGGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGAt                                               >  2:575314/1‑103 (MQ=255)

                                                                                                        aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGTGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGc           <  2:309132/139‑1 (MQ=255)

                                                                                                        aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGc           >  1:142139/1‑139 (MQ=255)

                                                                                                        aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGc           <  1:47930/139‑1 (MQ=255)

                                                                                                        aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGc           <  2:142139/139‑1 (MQ=255)

                                                                                                         ccATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCa          <  1:263549/139‑1 (MQ=255)

                                                                                                          cATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                           >  1:430561/1‑57 (MQ=255)

                                                                                                          cATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                           <  2:430561/57‑1 (MQ=255)

                                                                                                            tCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaagaa                                          <  1:326333/104‑1 (MQ=255)

                                                                                                            tCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaagaa                                          >  2:326333/1‑104 (MQ=255)

                                                                                                            tCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                            <  2:206321/118‑1 (MQ=255)

                                                                                                            tCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                            >  1:206321/1‑118 (MQ=255)

                                                                                                            tCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAgg       <  2:199007/139‑1 (MQ=255)

                                                                                                                 tACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                            <  1:208697/113‑1 (MQ=255)

                                                                                                                 tACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                            >  2:208697/1‑113 (MQ=255)

                                                                                                                 tACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACt  <  1:322848/139‑1 (MQ=255)

                                                                                                                   cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                              >  2:498171/1‑77 (MQ=255)

                                                                                                                   cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                              <  1:498171/77‑1 (MQ=255)

TGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACT  >  NC_000913/4297536‑4297787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A