Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,297,668 C→T D567N (GAC→AAC)  fdhF ← formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,297,6680CT100.0% 230.5 / NA 71D567N (GAC→AAC) fdhFformate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/35);  total (36/35)

TGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCC  >  NC_000913/4297543‑4297798
                                                                                                                             |                                                                                                                                    
tgCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTAcc                                                                                                                         >  2:676378/1‑139 (MQ=255)
     aGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑c                                                                                                                  >  2:1034221/1‑139 (MQ=255)
       ccAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGc                                                                                                                >  2:1272133/1‑139 (MQ=255)
          ggTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAgg                                                                                                             >  1:63210/1‑139 (MQ=255)
           gTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGAt                                                                                                                                   >  2:1759757/1‑118 (MQ=255)
           gTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGAt                                                                                                                                   <  1:1759757/118‑1 (MQ=255)
                tcaGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTgaaga                                                                                                       >  1:818754/1‑139 (MQ=255)
                 cagccagcGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTgaagaa                                                                                                      <  2:818754/139‑1 (MQ=255)
                 cagccagcGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTgaagaa                                                                                                      >  1:720952/1‑139 (MQ=255)
                 cagccagcGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTgaagaa                                                                                                      <  1:765840/139‑1 (MQ=255)
                     cagcGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCa                                                                                                                       >  1:1856932/1‑120 (MQ=255)
                     cagcGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCa                                                                                                                       <  2:1856932/120‑1 (MQ=255)
                      agcGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTgcg                                                                                                 <  2:63210/139‑1 (MQ=255)
                         gccgccAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCa                                                                                              >  1:1607496/1‑139 (MQ=255)
                           cgccAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGa                                                                                            <  2:1453641/139‑1 (MQ=255)
                                  gCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCg                                                                                     <  2:145345/139‑1 (MQ=255)
                                      cacaGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGa                                                                                            <  1:827397/128‑1 (MQ=255)
                                      cacaGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGa                                                                                            >  2:827397/1‑128 (MQ=255)
                                       acaGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTa                                                                                >  2:715894/1‑139 (MQ=255)
                                              ccGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                         <  1:1034221/139‑1 (MQ=255)
                                              ccGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                         <  2:1607496/139‑1 (MQ=255)
                                                   cATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCGCAGGTGAAGAACTGCGCCAGACCGTTCgggg                                                                                  >  2:1167431/1‑127 (MQ=255)
                                                   cATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCGCAGGTGAAGAACTGCGCCAGACCGTTCgggg                                                                                  <  1:1167431/127‑1 (MQ=255)
                                                     tCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCttt                                                                  >  2:481412/1‑139 (MQ=255)
                                                        aaCGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTaaa                                                               <  2:720952/139‑1 (MQ=255)
                                                               agagTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaa                                                        >  1:1705032/1‑139 (MQ=255)
                                                                gagTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaag                                                       <  1:715894/139‑1 (MQ=255)
                                                                  gtagtGACCAACTTCACGCACCGTTGATAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaagaa                                                     <  1:3878/139‑1 (MQ=255)
                                                                      tGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                         <  1:1032903/115‑1 (MQ=255)
                                                                      tGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                         >  2:1032903/1‑115 (MQ=255)
                                                                           aaCTTCACGCACCGTTGACAGTACCATCGGTTACTCGTCGGTGAGTTTGTTGATCGGCGCTACCCAGC‑‑AGCAGGTGAAGAAATGCGTCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACGGATAAGAAGTCCCCTGa                                            >  1:664430/1‑139 (MQ=255)
                                                                           aaCTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTgaagaa                                                                                                      <  1:628604/81‑1 (MQ=255)
                                                                           aaCTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTgaagaa                                                                                                      >  2:628604/1‑81 (MQ=255)
                                                                           aaCTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCg                                                                                     >  2:1676907/1‑98 (MQ=255)
                                                                           aaCTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCg                                                                                     <  1:256011/98‑1 (MQ=255)
                                                                           aaCTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCg                                                                                     >  2:256011/1‑98 (MQ=255)
                                                                           aaCTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCg                                                                                     <  1:1676907/98‑1 (MQ=255)
                                                                           aaCTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGa                                            >  2:1795833/1‑139 (MQ=255)
                                                                             cTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATc                                          >  2:686126/1‑139 (MQ=255)
                                                                                cACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                                       <  1:1795833/139‑1 (MQ=255)
                                                                                cACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                                       <  1:1272133/139‑1 (MQ=255)
                                                                                            aCAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATc                           >  2:643426/1‑139 (MQ=255)
                                                                                              aGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATcgc                         <  1:686126/139‑1 (MQ=255)
                                                                                                 aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATc                                          >  1:1806265/1‑119 (MQ=255)
                                                                                                 aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATc                                          <  2:1806265/119‑1 (MQ=255)
                                                                                                 aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGc                      >  2:460147/1‑139 (MQ=255)
                                                                                                 aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGc                      <  2:1786769/139‑1 (MQ=255)
                                                                                                   cATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAg                                                            >  2:1274575/1‑99 (MQ=255)
                                                                                                   cATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGAt                                                          <  2:448944/101‑1 (MQ=255)
                                                                                                   cATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGAt                                                          >  1:448944/1‑101 (MQ=255)
                                                                                                   cATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGCGGTATCAAACTTCTCTTTAAACAg                                                            <  1:1274575/99‑1 (MQ=255)
                                                                                                     tCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAg                                                                                             >  1:1612858/1‑64 (MQ=255)
                                                                                                     tCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAg                                                                                             <  2:1612858/64‑1 (MQ=255)
                                                                                                     tCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAgg                  <  2:369034/139‑1 (MQ=255)
                                                                                                     tCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAgg                  >  2:1531730/1‑139 (MQ=255)
                                                                                                     tCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAgg                  >  1:685261/1‑139 (MQ=255)
                                                                                                     tCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAgg                  <  1:643426/139‑1 (MQ=255)
                                                                                                      cGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaagaa                                                     <  2:1569967/103‑1 (MQ=255)
                                                                                                      cGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaagaa                                                     >  1:1569967/1‑103 (MQ=255)
                                                                                                        ggTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCTAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                                       <  1:1241801/115‑1 (MQ=255)
                                                                                                        ggTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTGTAAACAGATAAGAAGTCCCCTGATCGGc                                       >  2:1241801/1‑115 (MQ=255)
                                                                                                          tACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGa                                                                                            <  2:264860/60‑1 (MQ=255)
                                                                                                          tACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGa                                                                                            >  1:264860/1‑60 (MQ=255)
                                                                                                            cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTga                                                                                                          >  1:1278855/1‑44 (MQ=255)
                                                                                                            cTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTga                                                                                                          <  2:1278855/44‑1 (MQ=255)
                                                                                                               gtcgGTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACtgaatg        >  1:447611/1‑139 (MQ=255)
                                                                                                                   gTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCgg                       >  2:1056634/1‑120 (MQ=255)
                                                                                                                   gTGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCgg                       <  1:1056634/120‑1 (MQ=255)
                                                                                                                    tGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTgaagaa                                                                                                      >  2:166051/1‑40 (MQ=38)
                                                                                                                    tGAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTgaagaa                                                                                                      <  1:166051/40‑1 (MQ=38)
                                                                                                                     gAGTTTGTTGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGccc  <  2:685261/139‑1 (MQ=255)
                                                                                                                             |                                                                                                                                    
TGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGT‑‑CGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCC  >  NC_000913/4297543‑4297798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A