Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,447,869	Δ1 bp	coding (3758/3780 nt)	tamB →	translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,447,867	0	T	.	100.0%	218.1 / NA	51	coding (3756/3780 nt)	tamB	translocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (30/21); total (30/21)

CCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTA  >  NC_000913/4447745‑4448000

ccAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAt                                                                                                                                       <  1:279175/123‑1 (MQ=255)

ccAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAt                                                                                                                                       >  2:279175/1‑123 (MQ=255)

ccAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGa                                                                                                                      >  2:680603/1‑139 (MQ=255)

 cAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAg                                                                                                                     <  2:7307/139‑1 (MQ=255)

   ggTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGtt                                                                                                                   >  2:95870/1‑139 (MQ=255)

     tCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGtttt                                                                                                                 >  2:304492/1‑139 (MQ=255)

               aaaTACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGa                                                                                                       <  1:680603/139‑1 (MQ=255)

                      gCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTg                                                                                                >  1:654510/1‑139 (MQ=255)

                                    gACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTa                                                                                  <  2:566050/139‑1 (MQ=255)

                                    gACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTa                                                                                  <  1:95870/139‑1 (MQ=255)

                                     aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTaac                                                                                                              <  2:33773/110‑3 (MQ=255)

                                     aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTaac                                                                                                              >  1:33773/1‑108 (MQ=255)

                                         tataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCca                                                                             >  1:166216/1‑139 (MQ=255)

                                         tataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCca                                                                             >  1:190003/1‑139 (MQ=255)

                                           taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                           >  2:5306/1‑139 (MQ=255)

                                             gCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaa                                                                         <  1:304492/139‑1 (MQ=255)

                                              cAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                              <  2:309645/133‑1 (MQ=255)

                                              cAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                              >  1:309645/1‑133 (MQ=255)

                                                   cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGtt                                                                                                                         <  1:55665/85‑1 (MQ=255)

                                                   cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGtt                                                                                                                         >  2:55665/1‑85 (MQ=255)

                                                         ttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                             <  2:163082/139‑1 (MQ=255)

                                                                 tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTTGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                     <  1:747053/139‑1 (MQ=255)

                                                                  cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaaca                                                                       <  2:791600/120‑1 (MQ=255)

                                                                  cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaaca                                                                       >  1:791600/1‑120 (MQ=255)

                                                                  cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATg                                                    >  2:757450/1‑139 (MQ=255)

                                                                  cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAAAACAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATg                                                    >  1:401072/1‑139 (MQ=255)

                                                                      tGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCa                                                >  1:141810/1‑139 (MQ=255)

                                                                       gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                               <  2:141810/139‑1 (MQ=255)

                                                                        aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAAt                                              >  1:64707/1‑139 (MQ=255)

                                                                         tGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATg                                             <  1:773732/139‑1 (MQ=255)

                                                                           ccTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                           <  2:166216/139‑1 (MQ=255)

                                                                                     atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTgg                                 >  1:97937/1‑139 (MQ=255)

                                                                                          gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                            >  2:67775/1‑139 (MQ=255)

                                                                                          gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                            >  2:726509/1‑139 (MQ=255)

                                                                                          gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                            >  2:70207/1‑139 (MQ=255)

                                                                                              ccGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                        >  1:364194/1‑139 (MQ=255)

                                                                                               cGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAg                       >  1:521183/1‑139 (MQ=255)

                                                                                                       gtgtAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATa               <  2:97937/139‑1 (MQ=255)

                                                                                                            gACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                 <  2:339143/84‑1 (MQ=255)

                                                                                                            gACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                 >  1:339143/1‑84 (MQ=255)

                                                                                                             aCCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                           <  2:508521/73‑1 (MQ=255)

                                                                                                             aCCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                           >  1:508521/1‑73 (MQ=255)

                                                                                                              ccAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa        <  2:190003/139‑1 (MQ=255)

                                                                                                              ccAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa        <  1:757450/139‑1 (MQ=255)

                                                                                                                 ggCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt     >  1:384362/1‑139 (MQ=255)

                                                                                                                 ggCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt     >  1:360987/1‑139 (MQ=255)

                                                                                                                  gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCCGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg    >  1:146196/1‑139 (MQ=255)

                                                                                                                  gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                 <  2:581668/78‑1 (MQ=255)

                                                                                                                  gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                 >  1:581668/1‑78 (MQ=255)

                                                                                                                  gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg    >  2:219594/1‑139 (MQ=255)

                                                                                                                  gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg    >  2:103690/1‑139 (MQ=255)

                                                                                                                  gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg    >  1:381195/1‑139 (MQ=255)

                                                                                                                    aCTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta  <  2:568386/139‑1 (MQ=255)

CCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTA  >  NC_000913/4447745‑4448000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A