Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,308,868	G→A	C19C (TGC→TGT)	nlpI ←	lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,308,868	0	G	A	100.0%	161.3 / NA	50	C19C (TGC→TGT)	nlpI	lipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/27); total (23/27)

AGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCC  >  NC_000913/3308734‑3309003

aGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATc                                                                                                                                     <  2:1152917/139‑1 (MQ=255)

    gtgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACa                                                                                                                                       <  2:104848/133‑1 (MQ=255)

    gtgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGc                                                                                                                                 >  2:167271/1‑139 (MQ=255)

     tgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGTCCGGAA‑‑GTATTACTACATCCTGCa                                                                                                                                >  2:1150816/1‑139 (MQ=255)

     tgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCa                                                                                                                                >  2:152177/1‑139 (MQ=255)

     tgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCa                                                                                                                                <  2:1346759/139‑1 (MQ=255)

                     gTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTCCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCg                                                                                                                <  2:1358854/139‑1 (MQ=255)

                      ttAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                               >  2:588398/1‑139 (MQ=255)

                      ttAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                               <  1:335487/139‑1 (MQ=255)

                               gACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                               <  2:175607/130‑1 (MQ=255)

                               gACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                               >  1:175607/1‑130 (MQ=255)

                                aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCt                                                                                                                                   >  2:850896/1‑109 (MQ=255)

                                aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCt                                                                                                                                   <  1:850896/109‑1 (MQ=255)

                                aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGc                                                                                                                                 <  1:1536083/111‑1 (MQ=255)

                                aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGc                                                                                                                                 >  2:1536083/1‑111 (MQ=255)

                                aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGt                                                                                                                  <  2:1025838/126‑1 (MQ=255)

                                aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGt                                                                                                                  >  1:1025838/1‑126 (MQ=255)

                                aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAacac                                                                                                     >  1:625234/1‑139 (MQ=255)

                                aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAacac                                                                                                     <  1:152177/139‑1 (MQ=255)

                                aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAacac                                                                                                     <  1:167271/139‑1 (MQ=255)

                                    gCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAgc                                                                                                 <  2:967735/139‑1 (MQ=255)

                                                   aTACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCa                                                                                  >  2:812484/1‑139 (MQ=255)

                                                        tGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCATAAAAGGCTTCATTTcc                                                                             <  1:588398/139‑1 (MQ=255)

                                                         gCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTccc                                                                            >  1:94509/1‑139 (MQ=255)

                                                         gCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTccc                                                                            >  2:1916815/1‑139 (MQ=255)

                                                         gCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTccc                                                                            >  1:279536/1‑139 (MQ=255)

                                                         gCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTccc                                                                            >  1:1715797/1‑139 (MQ=255)

                                                          ccAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCa                                                                           <  2:625234/139‑1 (MQ=255)

                                                          ccAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCa                                                                           <  2:279536/139‑1 (MQ=255)

                                                                  aCTTCCTGCTGTAAAGTCGGTAGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGaa                                                                   <  1:1584206/139‑1 (MQ=255)

                                                                    ttCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCa                                                                           <  2:145130/129‑1 (MQ=255)

                                                                    ttCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCa                                                                           >  1:145130/1‑129 (MQ=255)

                                                                      ccTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGAcc                                                               <  2:1490090/139‑1 (MQ=255)

                                                                          ctgTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                               >  2:534146/1‑87 (MQ=255)

                                                                          ctgTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                               <  1:534146/87‑1 (MQ=255)

                                                                                    ggTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAAc                                                 >  2:861460/1‑139 (MQ=255)

                                                                                       tGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCa                                                                           <  1:1059255/110‑1 (MQ=255)

                                                                                       tGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCa                                                                           >  2:1059255/1‑110 (MQ=255)

                                                                                            tGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTGTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTc                                              <  2:647401/136‑1 (MQ=255)

                                                                                            tGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTGTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTc                                              >  1:647401/1‑136 (MQ=255)

                                                                                                  cgcgAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGt                                   <  1:812484/139‑1 (MQ=255)

                                                                                                      aGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCt                               >  2:1069472/1‑139 (MQ=255)

                                                                                                           ttCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACaa                          <  1:861460/139‑1 (MQ=255)

                                                                                                                  ttACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTtgaa                                                      <  2:178131/104‑1 (MQ=255)

                                                                                                                  ttACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTtgaa                                                      >  1:178131/1‑104 (MQ=255)

                                                                                                                  ttACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTACCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTcc                   >  1:1244587/1‑139 (MQ=255)

                                                                                                                     cGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAg                         >  2:1779815/1‑130 (MQ=255)

                                                                                                                     cGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAg                         <  1:1779815/130‑1 (MQ=255)

                                                                                                                      gCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGcc               <  2:94509/139‑1 (MQ=255)

                                                                                                                                     aCTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGccc  <  2:1715797/139‑1 (MQ=255)

AGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCC  >  NC_000913/3308734‑3309003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A