Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A36 F1 I0 R1	94	14.9	847684	89.6%	759524	133.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,450,733	C→A	100%	R45R (CGC→CGA)	gpD →	tail formation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,450,733	0	C	A	100.0%	55.0 / NA	17	R45R (CGC→CGA)	gpD	tail formation
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (9/8); total (9/8)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCACGTATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGCGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCAAGGGCTGACCCGCTTGGGCGCATACACCGTCGACG  >  NC_002947/3450606‑3450833

ccACGTATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGc                                                                                              >  1:174251/1‑136 (MQ=255)

ccACGTATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGc                                                                                              >  1:250132/1‑136 (MQ=255)

ccACGTATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGc                                                                                              >  1:60831/1‑136 (MQ=255)

     tATCAAATCGTCGCGTATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTg                                                                                         >  1:103696/1‑136 (MQ=255)

     tATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTg                                                                                         >  2:37437/1‑136 (MQ=255)

     tATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGACTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGCCGACCGAGACCAGGCTGTTg                                                                                         >  1:16584/1‑136 (MQ=255)

             cgtcgCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCg                                                                                 <  1:34661/136‑1 (MQ=255)

                      tGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGt                                                                        <  1:281535/136‑1 (MQ=255)

                       ggCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTa                                                                       <  1:11079/136‑1 (MQ=255)

                             gACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCTAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAAcg                                                                 <  2:417413/136‑1 (MQ=255)

                                               aaTGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCt                                               <  2:110522/136‑1 (MQ=255)

                                                    ccGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAg                                          >  1:403583/1‑136 (MQ=255)

                                                          gTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCaa                                    <  1:99384/136‑1 (MQ=255)

                                                                            ggACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCAAGGGCTGAccc                          >  2:290343/1‑128 (MQ=255)

                                                                            ggACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCAAGGGCTGAccc                          <  1:290343/128‑1 (MQ=255)

                                                                            ggACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCAAGGGCTGACCCGCTTGGgc                  >  2:251435/1‑136 (MQ=255)

                                                                                            gAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCAAGGGCTGACCCGCTTGGGCGCATACACCGTcgacg  <  1:323472/136‑1 (MQ=255)

CCACGTATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGCGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCAAGGGCTGACCCGCTTGGGCGCATACACCGTCGACG  >  NC_002947/3450606‑3450833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A