Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F85 I0 R1 2080 367.9 17641200 95.7% 16882628 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,590,436 C→G 19.9% Q17H (CAG→CAC)  folD‑II ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,590,4360CG19.9% 910.9 / 215.0 372Q17H (CAG→CAC) folD‑IIbifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base C (155/143);  new base G (41/33);  total (196/176)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 385 total aligned reads displayed.

GCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCGGGCAAATCCTCTAAC  >  NC_002947/2590287‑2590585
                                                                                                                                                      |                                                                                                                                                     
  cTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATctgctg                                                                                                                                                     <  1:2504475/150‑1 (MQ=255)
    gCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGgc                                                                                                                                                   >  1:236917/1‑150 (MQ=255)
    gCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGgc                                                                                                                                                   <  1:5415783/150‑1 (MQ=255)
         aTGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAgg                                                                                                                                              <  2:2616922/150‑1 (MQ=255)
          tGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGc                                                                                                                                             <  1:4423589/150‑1 (MQ=255)
          tGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGc                                                                                                                                             >  1:5006534/1‑150 (MQ=255)
            aaGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTg                                                                                                                                           >  1:116002/1‑150 (MQ=255)
             aGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTgg                                                                                                                                          <  2:4257285/150‑1 (MQ=255)
             aGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTgg                                                                                                                                          >  2:5184399/1‑150 (MQ=255)
              gCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTggc                                                                                                                                         >  1:6175839/1‑150 (MQ=255)
               ccgcccgcTGCGCAGTCCGTGCGCGTGTGCGAGACATCGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTCCG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTggcg                                                                                                                                        <  2:5516577/150‑1 (MQ=255)
               ccGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTggcg                                                                                                                                        >  2:8624577/1‑150 (MQ=255)
               ccGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTggcg                                                                                                                                        >  2:7540180/1‑150 (MQ=255)
                cGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTggcgg                                                                                                                                       <  2:1843366/150‑1 (MQ=255)
                 gACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTggcggc                                                                                                                                      <  2:5315508/150‑1 (MQ=255)
                 gACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTggcggc                                                                                                                                      <  1:8543682/150‑1 (MQ=255)
                    ctctTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGAt                                                                                                                                   >  1:132359/1‑150 (MQ=255)
                      ctTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAg                                                                                                                                 >  1:8084238/1‑150 (MQ=255)
                       ttCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGc                                                                                                                                >  1:731657/1‑150 (MQ=255)
                       ttCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGc                                                                                                                                >  2:3270020/1‑150 (MQ=255)
                        tCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGcc                                                                                                                               >  1:4724950/1‑150 (MQ=255)
                          gCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCtt                                                                                                                             <  1:5671835/150‑1 (MQ=255)
                          gCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCtt                                                                                                                             >  1:2653711/1‑150 (MQ=255)
                           cAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTg                                                                                                                            >  2:4450384/1‑150 (MQ=255)
                           cAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTg                                                                                                                            <  2:4623951/150‑1 (MQ=255)
                           cAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTg                                                                                                                            <  2:237430/150‑1 (MQ=255)
                              tCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCa                                                                                                                         >  1:1816199/1‑150 (MQ=255)
                               ccTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCAt                                                                                                                        <  2:795066/150‑1 (MQ=255)
                                cTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATc                                                                                                                       <  2:3740997/150‑1 (MQ=255)
                                cTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATc                                                                                                                       <  2:5422610/150‑1 (MQ=255)
                                cTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATc                                                                                                                       >  2:1874128/1‑150 (MQ=255)
                                 ttGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCg                                                                                                                      >  2:3652901/1‑150 (MQ=255)
                                  tGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGa                                                                                                                     <  1:598433/150‑1 (MQ=255)
                                     gcTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTa                                                                                                                  <  2:3483590/150‑1 (MQ=255)
                                      cTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAg                                                                                                                 >  1:1942319/1‑150 (MQ=255)
                                       ttGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAgg                                                                                                                <  1:387691/150‑1 (MQ=255)
                                           gCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGtgtg                                                                                                            >  2:5247657/1‑150 (MQ=255)
                                            cGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGc                                                                                                           >  1:3319823/1‑150 (MQ=255)
                                                aCATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTc                                                                                                       <  1:1856566/150‑1 (MQ=255)
                                                  ntAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCAt                                                                                                     >  1:4937076/2‑150 (MQ=255)
                                                  aTAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCAt                                                                                                     <  1:862517/150‑1 (MQ=255)
                                                  aTAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCAt                                                                                                     >  2:4639170/1‑150 (MQ=255)
                                                   tAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATa                                                                                                    >  1:5319171/1‑150 (MQ=255)
                                                   tAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATa                                                                                                    >  2:2214931/1‑150 (MQ=255)
                                                     gACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGa                                                                                                  <  1:8460921/150‑1 (MQ=255)
                                                       cTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGAcg                                                                                                >  2:6104320/1‑150 (MQ=255)
                                                        ttGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGAcgc                                                                                               >  1:691239/1‑150 (MQ=255)
                                                          gggAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGt                                                                                             >  1:7817243/1‑150 (MQ=255)
                                                            gAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGa                                                                                           <  1:6376565/150‑1 (MQ=255)
                                                                cctggTCGGCACCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTaa                                                                                       >  1:6474283/4‑150 (MQ=255)
                                                                 ggggTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGgtgt                                                                                                             >  2:4757453/1‑127 (MQ=255)
                                                                  tggTCGGCACCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAAcc                                                                                     >  1:6700/2‑150 (MQ=255)
                                                                  gggTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAAcc                                                                                     <  1:160708/150‑1 (MQ=255)
                                                                    gTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCAt                                                                                   <  1:358179/150‑1 (MQ=255)
                                                                     tCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATc                                                                                  >  2:8107261/1‑150 (MQ=255)
                                                                     tCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATc                                                                                  <  2:8621135/150‑1 (MQ=255)
                                                                      cGGTGCCGACCAGGGTCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCg                                                                                 <  1:1005231/150‑1 (MQ=255)
                                                                       ggTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCga                                                                                <  2:8733282/150‑1 (MQ=255)
                                                                        gTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCgag                                                                               >  1:836596/1‑150 (MQ=255)
                                                                           ccGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAg                                                                            <  1:4140356/150‑1 (MQ=255)
                                                                           ccGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAg                                                                            <  1:2102769/150‑1 (MQ=255)
                                                                            cGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAgg                                                                           <  1:1064165/150‑1 (MQ=255)
                                                                             gACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGa                                                                          >  1:994888/1‑150 (MQ=255)
                                                                             gACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGa                                                                          <  1:7015269/150‑1 (MQ=255)
                                                                               ccAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAAc                                                                        >  1:3975067/1‑150 (MQ=255)
                                                                                 aGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACaa                                                                      <  2:5003863/150‑1 (MQ=255)
                                                                                     tCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATaaa                                                                  <  1:6807818/150‑1 (MQ=255)
                                                                                     tCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATaaa                                                                  <  2:6784838/150‑1 (MQ=255)
                                                                                     tCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATaaa                                                                  <  2:641468/150‑1 (MQ=255)
                                                                                        cTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGaa                                                               <  1:4223725/150‑1 (MQ=255)
                                                                                        cTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGaa                                                               <  1:4956631/150‑1 (MQ=255)
                                                                                        cTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGaa                                                               >  2:4471802/1‑150 (MQ=255)
                                                                                        cTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGaa                                                               >  1:8732558/1‑150 (MQ=255)
                                                                                         tGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAAc                                                              >  1:514799/1‑150 (MQ=255)
                                                                                           acAGGCCCGGCGTACG‑CAGGCCTTGCAGGCGACGCTCAACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCTATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAATAACGt                                                            >  2:3137904/2‑150 (MQ=255)
                                                                                             aaggcctgCGTACGCCGGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTg                                                           >  2:7053264/8‑150 (MQ=255)
                                                                                             aGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGc                                                          >  1:5549170/1‑150 (MQ=255)
                                                                                             aGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGc                                                          <  2:6233365/150‑1 (MQ=255)
                                                                                               gCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCAt                                                        >  2:1172258/1‑150 (MQ=255)
                                                                                               gCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCAt                                                        >  1:2761523/1‑150 (MQ=255)
                                                                                                   ggCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATtctc                                                    <  2:6611869/150‑1 (MQ=255)
                                                                                                      gTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCa                                                 >  1:2218450/1‑150 (MQ=255)
                                                                                                      gTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGAATTCTCGCa                                                 >  2:112679/1‑150 (MQ=255)
                                                                                                          g‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGcc                                                                                                                               <  2:156784/68‑1 (MQ=255)
                                                                                                            cAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGACTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACa                                            <  2:2261983/150‑1 (MQ=255)
                                                                                                               gCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATgg                                         <  2:8423696/150‑1 (MQ=255)
                                                                                                               gCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATgg                                         <  2:3898313/150‑1 (MQ=255)
                                                                                                                ccTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATggg                                        >  2:5711144/1‑150 (MQ=255)
                                                                                                                    gCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCaa                                    <  2:6525255/150‑1 (MQ=255)
                                                                                                                    gCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCaa                                    <  2:6964830/150‑1 (MQ=255)
                                                                                                                        gCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTa                                                                                                                  <  1:3119152/68‑1 (MQ=255)
                                                                                                                        gCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGc                                >  1:3520671/1‑150 (MQ=255)
                                                                                                                           aCGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCaaa                             <  1:7492044/150‑1 (MQ=255)
                                                                                                                               tCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCg                         >  1:1468036/1‑150 (MQ=255)
                                                                                                                                  aCGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGggt                      >  1:5235348/1‑150 (MQ=255)
                                                                                                                                    gACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGggtgg                    >  2:7663332/1‑150 (MQ=255)
                                                                                                                                         gTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCggg               >  2:7179719/1‑150 (MQ=255)
                                                                                                                                         gTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCggg               >  2:3006094/1‑150 (MQ=255)
                                                                                                                                         gTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCggg               >  1:8606951/1‑150 (MQ=255)
                                                                                                                                              gCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGCTTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCGGGCtaag          <  2:140670/150‑2 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACG‑CAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCGGGCAAATCCTCTAAC  >  NC_002947/2590287‑2590585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A