Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F85 I1 R1 23 334.9 15793868 95.8% 15130525 148.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 2,590,436 C→G Q17H (CAG→CAC)  folD‑II ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,590,4360CG98.5% 930.4 / ‑0.3 264Q17H (CAG→CAC) folD‑IIbifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base G (144/116);  total (147/117)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

CTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCGGGCAAATCCTC  >  NC_002947/2590289‑2590581
                                                                                                                                                   |                                                                                                                                                 
cTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATctggtg                                                                                                                                                 <  2:7499905/150‑1 (MQ=255)
cTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATctggtg                                                                                                                                                 <  1:280587/150‑1 (MQ=255)
  gCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGgc                                                                                                                                               >  1:355193/1‑150 (MQ=255)
  gCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGgc                                                                                                                                               <  1:1934143/150‑1 (MQ=255)
     aaaTGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCa                                                                                                                                            <  2:1260622/150‑1 (MQ=255)
     aaaTGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACAAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCa                                                                                                                                            <  2:7706272/150‑1 (MQ=255)
           aGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTgg                                                                                                                                      >  2:3482988/1‑150 (MQ=255)
           aGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTgg                                                                                                                                      <  1:5806908/150‑1 (MQ=255)
            gCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTggc                                                                                                                                     >  1:4978176/1‑150 (MQ=255)
                 ccTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGa                                                                                                                                <  1:7267502/150‑1 (MQ=255)
                  ctctTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGAt                                                                                                                               >  1:1783463/1‑150 (MQ=255)
                  ctctTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGAt                                                                                                                               >  1:3358056/1‑150 (MQ=255)
                   tctTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATa                                                                                                                              <  1:4813181/150‑1 (MQ=255)
                   tctTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATa                                                                                                                              >  2:3104898/1‑150 (MQ=255)
                   tctTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATa                                                                                                                              <  2:1782701/150‑1 (MQ=255)
                    ctTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAg                                                                                                                             <  1:7495379/150‑1 (MQ=255)
                     ttCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGc                                                                                                                            >  2:6930129/1‑150 (MQ=255)
                     ttCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGc                                                                                                                            <  2:1975348/150‑1 (MQ=255)
                       cGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCATGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCt                                                                                                                          <  1:636154/150‑1 (MQ=255)
                        gCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCtt                                                                                                                         <  1:1596201/150‑1 (MQ=255)
                          aGTCCTTTCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGc                                                                                                                       <  2:6861024/150‑1 (MQ=255)
                          aGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGc                                                                                                                       <  2:2199936/150‑1 (MQ=255)
                               ttGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCg                                                                                                                  >  2:7451082/1‑150 (MQ=255)
                                   gcTTGTGCGAGACATAGACTTGGGAGGCGGGGTTGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTa                                                                                                              <  1:5888455/150‑1 (MQ=255)
                                   gcTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTa                                                                                                              <  1:6759870/150‑1 (MQ=255)
                                    cTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAg                                                                                                             <  1:6858711/150‑1 (MQ=255)
                                    cTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAg                                                                                                             <  1:5465487/150‑1 (MQ=255)
                                     ttGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAgg                                                                                                            <  2:7300981/150‑1 (MQ=255)
                                     ttGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAgg                                                                                                            >  1:6602560/1‑150 (MQ=255)
                                        tgCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGgtgt                                                                                                         <  2:5350104/150‑1 (MQ=255)
                                          cGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGc                                                                                                       >  2:3059775/1‑150 (MQ=255)
                                          cGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGc                                                                                                       >  1:6873884/1‑150 (MQ=255)
                                           gagaCATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCa                                                                                                      >  2:5313343/1‑150 (MQ=255)
                                           gagaCATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCa                                                                                                      <  2:7581372/150‑1 (MQ=255)
                                           gagaCATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCa                                                                                                      <  1:7063252/150‑1 (MQ=255)
                                               cATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCa                                                                                                  <  1:6096811/150‑1 (MQ=255)
                                                aTAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCAt                                                                                                 <  2:3539319/150‑1 (MQ=255)
                                                aTAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCAt                                                                                                 >  2:1726541/1‑150 (MQ=255)
                                                aTAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCAt                                                                                                 >  1:1756889/1‑150 (MQ=255)
                                                 tAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATa                                                                                                <  2:2758049/150‑1 (MQ=255)
                                                 tAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATa                                                                                                <  2:4315767/150‑1 (MQ=255)
                                                   gACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGa                                                                                              <  1:3489877/150‑1 (MQ=255)
                                                      ttGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGAcgc                                                                                           <  1:4363192/150‑1 (MQ=255)
                                                      ttGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGAcgc                                                                                           >  2:7008852/1‑150 (MQ=255)
                                                      ttGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGAcgc                                                                                           >  1:917268/1‑150 (MQ=255)
                                                       tGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGAcgcg                                                                                          <  2:101337/150‑1 (MQ=255)
                                                        gggAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGt                                                                                         <  1:7598332/150‑1 (MQ=255)
                                                        gggAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGt                                                                                         <  2:6486133/150‑1 (MQ=255)
                                                           aGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGAt                                                                                      >  2:6792128/1‑150 (MQ=255)
                                                           aGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGAt                                                                                      >  2:6792095/1‑150 (MQ=255)
                                                           aGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGAAAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGAt                                                                                      >  2:6220324/1‑150 (MQ=255)
                                                             gCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTa                                                                                    >  2:5547170/1‑150 (MQ=255)
                                                              cctggTCGGCACCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTaa                                                                                   >  1:1923551/4‑150 (MQ=255)
                                                               ctggTCGGCACCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAAc                                                                                  >  1:1010564/3‑150 (MQ=255)
                                                                  gTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCAt                                                                               >  1:5304190/1‑150 (MQ=255)
                                                                   tCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATc                                                                              <  2:5106456/150‑1 (MQ=255)
                                                                        gCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGaa                                                                         <  2:5924585/150‑1 (MQ=255)
                                                                          cGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAgg                                                                       <  2:4867538/150‑1 (MQ=255)
                                                                            aCCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGaa                                                                     <  2:5894398/150‑1 (MQ=255)
                                                                             ccAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACCCGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATCGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAAc                                                                    <  2:575477/150‑1 (MQ=255)
                                                                              cAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACa                                                                   <  2:3989929/150‑1 (MQ=255)
                                                                                 gATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATa                                                                >  1:1729034/1‑150 (MQ=255)
                                                                                   tCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGTCGCTCCACGTCACGTTGAGCGATCTGGTGGCGCTGGCTGGCGGCGATAGCCTTGCAATCGATTAGGTGTGCAGTCATAGACGCGTGTTTAAACATCGTGAAGGATCAATtaa                                                              <  2:1277425/150‑1 (MQ=255)
                                                                                   tCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATaaa                                                              >  1:4757105/1‑150 (MQ=255)
                                                                                    cACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAg                                                             >  2:5868892/1‑150 (MQ=255)
                                                                                     aCTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGa                                                            >  1:4524043/1‑150 (MQ=255)
                                                                                       tGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAAc                                                          >  1:7140139/1‑150 (MQ=255)
                                                                                         ccAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGt                                                        >  1:521602/1‑150 (MQ=255)
                                                                                           aGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGc                                                      <  2:3506253/150‑1 (MQ=255)
                                                                                                 ggCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATtctc                                                <  1:131109/150‑1 (MQ=255)
                                                                                                    gTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCa                                             <  2:4735693/150‑1 (MQ=255)
                                                                                                     tACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGaa                                                                         >  2:7261086/1‑121 (MQ=255)
                                                                                                       gccgggCCTGGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGTCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGa                                          >  2:2248852/5‑150 (MQ=255)
                                                                                                          aGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACAt                                       <  1:2959957/150‑1 (MQ=255)
                                                                                                          aGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACAt                                       >  2:4032282/1‑150 (MQ=255)
                                                                                                            gCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATgg                                     <  2:747094/150‑1 (MQ=255)
                                                                                                             ccTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGaa                                                           <  2:1514573/127‑1 (MQ=255)
                                                                                                             ccTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGaa                                                           >  1:1514573/1‑127 (MQ=255)
                                                                                                                 gCTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCaa                                <  2:1660997/150‑1 (MQ=255)
                                                                                                                  cTGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAg                               >  2:4484011/1‑150 (MQ=255)
                                                                                                                   tGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAgg                              <  1:1120828/150‑1 (MQ=255)
                                                                                                                   tGGCGACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAgg                              >  1:2543043/1‑150 (MQ=255)
                                                                                                                     gCGACGCTCCACGACACCTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCAACGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGc                            <  2:3662829/150‑1 (MQ=255)
                                                                                                                       gACGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCaa                          >  2:7410438/1‑150 (MQ=255)
                                                                                                                        aCGCTCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCaaa                         <  2:327106/150‑1 (MQ=255)
                                                                                                                            tCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCg                     >  1:7177756/1‑150 (MQ=255)
                                                                                                                            tCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCg                     >  1:5715422/1‑150 (MQ=255)
                                                                                                                            tCCACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCg                     >  1:5120002/1‑150 (MQ=255)
                                                                                                                             ccACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCgg                    <  2:3686500/150‑1 (MQ=255)
                                                                                                                              cACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCggg                   >  1:777207/1‑150 (MQ=255)
                                                                                                                              cACGACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCggg                   <  1:5949436/150‑1 (MQ=255)
                                                                                                                              cACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCggg                   >  2:5064315/1‑150 (MQ=255)
                                                                                                                                 gACACGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGggtgg                >  1:4138512/1‑150 (MQ=255)
                                                                                                                                   cacGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTc              <  1:6944929/150‑1 (MQ=255)
                                                                                                                                     cGTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCgg            <  2:1108471/150‑1 (MQ=255)
                                                                                                                                      gTTGAGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCggg           >  1:2183485/1‑150 (MQ=255)
                                                                                                                                          aGCGATCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCGGGCaaa       >  1:3892936/1‑150 (MQ=255)
                                                                                                                                              aTCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCGGGCAAATCct   <  1:7363203/150‑1 (MQ=255)
                                                                                                                                              aTCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCGGGCAAATCct   <  1:242796/150‑1 (MQ=255)
                                                                                                                                               tCTGGTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCGGGCAAATCctc  >  2:4125180/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATCGAGAAGGAACAATAAAGAACGTGCATTCTCGCACGACATGGGCCAAGGGCAAAGGCGGGTGGTCGGGCAAATCCTC  >  NC_002947/2590289‑2590581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A