Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A36 F1 I4 R1	20	34.3	1939470	89.7%	1739704	133.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,154,508	T→C	D335G (GAC→GGC)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,154,508	0	T	C	100.0%	161.1 / NA	43	D335G (GAC→GGC)	rne	ribonuclease E
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/15); total (28/15)

AACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGACACCAGGGCTTCGGTC  >  NC_002947/2154393‑2154636

aaCCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTggcgg                                                                                                              <  2:148982/136‑1 (MQ=255)

aaCCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTggcgg                                                                                                              >  2:615790/1‑136 (MQ=255)

           gggCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTc                                                                                                   <  1:488961/136‑1 (MQ=255)

                cgGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCg                                                                                              <  1:438581/136‑1 (MQ=255)

                 ggTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGc                                                                                             >  1:285465/1‑136 (MQ=255)

                   tCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCtt                                                                                           >  2:216859/1‑136 (MQ=255)

                   tCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCtt                                                                                           >  2:822167/1‑136 (MQ=255)

                                aTTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTg                                                                              >  2:702427/1‑136 (MQ=255)

                                aTTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTg                                                                              >  1:581483/1‑136 (MQ=255)

                                    acgcacgcGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAgg                                                                          >  1:743695/1‑136 (MQ=255)

                                      gcacgcGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGc                                                                        <  1:372725/136‑1 (MQ=255)

                                                   aaCAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGGTTCTTCGATg                                                           <  1:29987/136‑1 (MQ=255)

                                                     cAGCGCGCTGGTTCTTCGCCGGGTTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTc                                                         <  2:594068/136‑1 (MQ=255)

                                                           gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTc                                                         <  1:178195/130‑1 (MQ=255)

                                                           gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTc                                                         >  2:178195/1‑130 (MQ=255)

                                                           gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTgtc                                                   >  2:37999/1‑134 (MQ=255)

                                                           gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  2:599627/1‑136 (MQ=255)

                                                           gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  2:511079/1‑136 (MQ=255)

                                                           gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  2:493679/1‑136 (MQ=255)

                                                           gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  2:337922/1‑136 (MQ=255)

                                                           gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  2:898026/1‑136 (MQ=255)

                                                           gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  1:461862/1‑136 (MQ=255)

                                                           gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  1:48477/1‑136 (MQ=255)

                                                           gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGAAGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCCCTTCCAGGGAGGACTGCAGCGCGGTTTCTAAGAAGACGCTGcc                                                   >  2:775174/1‑136 (MQ=255)

                                                             tGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCtt                                                 >  2:18423/1‑136 (MQ=255)

                                                             tGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCtt                                                 >  2:232848/1‑136 (MQ=255)

                                                             tGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCtt                                                 >  1:252564/1‑136 (MQ=255)

                                                             tGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCACGGCGGTTTCTTCGATGTCGCTGCCtt                                                 >  1:438812/1‑136 (MQ=255)

                                                                ttcttcGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTtgg                                              <  1:24733/136‑1 (MQ=255)

                                                                  cttcGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTtggtg                                            <  2:241210/136‑1 (MQ=255)

                                                                  cttcGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTtggtg                                            >  2:662351/1‑136 (MQ=255)

                                                                       ccGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGgcgcg                                       <  2:227905/136‑1 (MQ=255)

                                                                       ccGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTGCTTCGATGTCGCTGCCTTTGGTGgcgcg                                       <  2:562434/136‑1 (MQ=255)

                                                                        cGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGgcgcgc                                      >  1:594407/1‑136 (MQ=255)

                                                                        cGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGgcgcgc                                      >  1:644506/1‑136 (MQ=255)

                                                                                  tCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTgt                            >  2:569411/1‑135 (MQ=255)

                                                                                      tGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGtc                        <  1:695162/136‑1 (MQ=255)

                                                                                       taaGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGtcg                       <  1:548044/135‑1 (MQ=255)

                                                                                          gTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGtcgata                    >  1:790470/1‑135 (MQ=255)

                                                                                             gATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGac                 <  2:801857/136‑1 (MQ=255)

                                                                                              aTGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGacc                >  2:68356/1‑135 (MQ=255)

                                                                                               tGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGacac               >  2:468757/1‑136 (MQ=255)

                                                                                                            gccgATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGACACCAGGGCTtcggtc  <  2:581483/136‑1 (MQ=255)

AACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGACACCAGGGCTTCGGTC  >  NC_002947/2154393‑2154636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A