Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A36 F1 I3 R1 19 18.3 1069718 89.3% 955258 133.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 2,533,181 G→A A30A (GCG→GCA)  PP_2217 → enoyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,533,1810GA100.0% 52.5 / NA 16A30A (GCG→GCA) PP_2217enoyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/9);  total (7/9)

CATTGCGCGCAATCTTTGAAGGAGCAGACTGCATGGCATTCGAAACCATCCTGTTGGACATCCACGGCAAGGTTGGCCTGATCACCCTCAACCGGCCGCAGGCGCTCAATGCGCTGAACGCGCAGATTGTCGGCGAGATCAACCAGGCTCTGGACCAGCTCGAGCGCGACCCGAACATCGGCTGCGTGGTGCTGACAGGCTCGGCCAAAGCCTTTGCCGCTGGCGCCGACATCAAGGAAATGGCCGAGCTGCAA  >  NC_002947/2533060‑2533313
                                                                                                                         |                                                                                                                                    
cATTGCGCGCAATCTTTGAAGGAGCAGACTGCATGGCATTCGAAACCATCCTGTTGGACATCCACGGCAAGGTTGGCCTGATCACCCTCAACCGGCCGCAGGCGCTCAATGCGCTGAACGCACAGATTGTCGGCga                                                                                                                        >  2:409863/1‑136 (MQ=255)
      gcgcAATCTTTGAAGGAGCAGACTGCATGGCATTCGAAACCATCCTGTTGGACATCCACGGCAAGGTTGGCCTGATCACCCTCAACCGGCCGCAGGCGCTCAATGCGCTGAACGCACAGATTGTCGGCGAGATCaa                                                                                                                  <  2:520226/136‑1 (MQ=255)
          aaTCTTTGAAGGAGCAGACTGCATGGCATTCGAAACCATCCTGTTGGACATCCACGGCAAGGTTGGCCTGATCACCCTCAACCGGCCGCAGGCGCTCAATGCGCTGAACGCACAGATTGTCGGCGAGATCAACCAg                                                                                                              <  1:360535/136‑1 (MQ=255)
            tCTTTGAAGGAGCAGACTGCATGGCATTCGAAACCATCCTGTTGGACATCCACGGCAAGGTTGGCCTGATCACCCTCAACCGGCCGCAGGCGCTCAATGCGCTGAACGCACAGATTGTCGGCGAGATCAACCAGGc                                                                                                            >  1:404119/1‑136 (MQ=255)
                tGAAGGAGCAGACTGCATGGCATTCGAAACCATCCTGTTGGACATCCACGGCAAGGTTGGCCTGATCACCCTCAACCGGCCGCAGGCGCTCAATGCGCTGAACGCACAGATTGTCGGCGAGATCAACCAGGCTCTg                                                                                                        <  2:404119/136‑1 (MQ=255)
                          gACTGCATGGCATTCGAAACCATCCTGTTGGACATCCACGGCAAGGTTGGCCTGATCACCCTCAACCGGCCGCAGGCGCTCAATGCGCTGAACGCACAGATTGTCGGCGAGATCAACCAGGCTCTGGACCAGCTCg                                                                                              >  1:440825/1‑136 (MQ=255)
                                   gCATTCGAAACCATCCTGTTGGACATCCACGGCAAGGTTGGCCTGATCACCCTCAACCGGCCGCAGGCGCTCAATGCGCTGAACGCACAGATTGTCGGCGAGATCAACCAGGCTCTGGACCAGCTCGAGCGCGAcc                                                                                     >  2:396727/1‑136 (MQ=255)
                                   gCATTCGAAACCATCCTGTTGGACATCCACGGCAAGGTTGGCCTGATCACCCTCAACCGGCCGCAGGCGCTCAATGCGCTGAACGCACAGATTGTCGGCGAGATCAACCAGGCTCTGGACCAGCTCGAGCGCGAcc                                                                                     >  2:331522/1‑136 (MQ=255)
                                            aCCATCCTGTTGGACATCCACGGCAAGGTTGGCCTGATCACCCTCAACCGGCCGCAGGCGCTCAATGCGCTGAACGCACAGATTGTCGGCAAGATCAACCAGGCTCTGGACCAGCTCGAGCGCGACCCGAACATCg                                                                            >  2:531822/1‑136 (MQ=255)
                                                              cACGGCAAGGTTGGCCTGATCACCCTCAACCGGCCGCAGGCGCTCAATGCGCTGAACGCACAGATTGTCGGCAAGATCAACCAGGCTCTGGACCAGCTCGAGCGCGACCCGAACATCGGCTGCGTGGTGCTGACAg                                                          <  1:531822/136‑1 (MQ=255)
                                                                                                      cgcTCAATGCGCTGAACGCACAGATTGTCGGCGAGATCAACCAGGCTCTg                                                                                                        <  1:504866/50‑1 (MQ=255)
                                                                                                      cgcTCAATGCGCTGAACGCACAGATTGTCGGCGAGATCAACCAGGCTCTg                                                                                                        >  2:504866/1‑50 (MQ=255)
                                                                                                        cTCAATGCGCTGAACGCACAGATTGTCGGCGAGATCAACCAGGCTCTGGACCAGCTCGAGCGCGACCCGAACATCGGCTGCGTGGTGCTGACAGGCTCGGCCAAAGCCTTTGCCGCTGGCGCCGACATCAAGGaaa                <  1:17385/136‑1 (MQ=255)
                                                                                                        cTCAATGCGCTGAACGCACAGATTGTCGGCGAGATCAACCAGGCTCTGGACCAGCTCGAGCGCGACCCGAACATCGGCTGCGTGGTGCTGACAGGCTCGGCCAAAGCCTTTGCCGCTGGCGCCGACATCAAGGaaa                <  1:369770/136‑1 (MQ=255)
                                                                                                        cTCAATGCGCTGAACGCACAGATTGTCGGCGAGATCAACCAGGCTCTGGACCAGCTCGAGCGCGACCCGAACATCGGCTGCGTGGTGCTGACAGGCTCGGCCAAAGCCTTTGCCGCTGGCGCCGACATCAAGGaaa                <  1:189989/136‑1 (MQ=255)
                                                                                                                    aaCGCACAGATTGTCGGCGAGATCAACCAGGCTCTGGACCAGCTCGAGCGCGACCCGAACATCGGCTGCGTGGTGCTGACAGGCTCGGCCAAAGCCTTTGCCGCTGGCGCCGACATCAAGGAAATGGCCGAGCTGc    <  1:267415/136‑1 (MQ=255)
                                                                                                                      cgcaCAGATTGTCGGCGAGATCAACCAGGCTCTGGACCAGCTCGAGCGCGACCCGAACATCGGCTGCGTGGTGCTGACAGGCTCGGCCAAAGCCTTTGCCGCTGGCGCCGACATCAAGGAAATGGCCGAGCTGCaa  >  2:273074/1‑136 (MQ=255)
                                                                                                                         |                                                                                                                                    
CATTGCGCGCAATCTTTGAAGGAGCAGACTGCATGGCATTCGAAACCATCCTGTTGGACATCCACGGCAAGGTTGGCCTGATCACCCTCAACCGGCCGCAGGCGCTCAATGCGCTGAACGCGCAGATTGTCGGCGAGATCAACCAGGCTCTGGACCAGCTCGAGCGCGACCCGAACATCGGCTGCGTGGTGCTGACAGGCTCGGCCAAAGCCTTTGCCGCTGGCGCCGACATCAAGGAAATGGCCGAGCTGCAA  >  NC_002947/2533060‑2533313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A