Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F110 I2 R1
|
19 |
56.9 |
2036372 |
91.3% |
1859207 |
142.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,296,381 |
+GC |
intergenic (+587/+55) |
gltP → / ← yjcO |
glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,296,380 | 1 | . | C | 90.2%
| 109.3
/ 7.6
| 41 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (3/1); new base C (18/19); total (21/20) |
| Fisher's exact test for biased strand distribution p-value = 6.06e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | NC_000913 | 4,296,380 | 2 | . | G | 90.2%
| 110.3
/ 7.6
| 41 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (3/1); new base G (18/19); total (21/20) |
| Fisher's exact test for biased strand distribution p-value = 6.06e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCA > NC_000913/4296265‑4296507
||
ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGTCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCCTTT‑‑A‑‑CCAC‑GCACCCCACATCGATGcccgcag > 2:654111/1‑138 (MQ=11)
aCGCGTCTCATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtt < 1:194617/143‑1 (MQ=255)
cgcgTCGTCTCAGGCCTACGCCAGACAGCGCAATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCACCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg < 2:700930/143‑1 (MQ=11)
gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAACCGTTgg > 1:342975/1‑143 (MQ=255)
gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg > 2:356211/1‑143 (MQ=255)
cTTCTCAGGCCTACGGCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGGAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa < 1:285358/143‑1 (MQ=11)
cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa < 1:493916/143‑1 (MQ=255)
aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttt > 1:960790/1‑143 (MQ=255)
aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttt > 1:389800/1‑143 (MQ=255)
aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGACAAGCCGTCTACA‑‑C‑‑C‑CCATCCGACATCAATGCCTGATTCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttt > 1:992587/1‑143 (MQ=14)
ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTTGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑Ca < 1:217217/143‑1 (MQ=14)
ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑Ca < 2:610455/143‑1 (MQ=255)
ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTCACTTTTT‑Ca > 2:820253/1‑143 (MQ=14)
gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑Caa < 1:42367/143‑1 (MQ=255)
cgcccGCCAGTCGGCGCAATAGCAGGATCTAGCTGGATTGTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑Caaa < 2:204982/139‑1 (MQ=14)
tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAAct < 1:709735/143‑1 (MQ=255)
tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAAct < 1:708701/143‑1 (MQ=255)
tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGCCCTATCTTAACCGTTGGTTAATTTTT‑CAAAct > 2:315313/1‑143 (MQ=255)
gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCtt < 2:86838/143‑1 (MQ=255)
gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCtt < 1:417529/143‑1 (MQ=255)
gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCtt < 1:118656/143‑1 (MQ=255)
ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGCTGGTTAATTTTT‑CCAACTCTTc > 2:471367/1‑143 (MQ=14)
aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTcac > 1:474890/1‑143 (MQ=255)
aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTGT‑CAAACTCTTcac > 1:856901/1‑143 (MQ=255)
gctatggCCTGATTTCGCGTGATGTCGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGt < 2:196000/137‑1 (MQ=17)
gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGt < 1:745113/143‑1 (MQ=255)
aaTAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGTAt < 1:324239/143‑1 (MQ=255)
gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAGTTTTT‑CCAACTCTTCCCACCCGGTATCAAAccat > 1:49048/1‑141 (MQ=18)
tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGTATCAAACCCTTc < 1:381308/143‑1 (MQ=255)
aGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATTAGGCCTATCTTAACCGTTGGTTAATTTTT‑TAAACTCTTCACACCCGGTATCAAACCATTCCAt > 1:502429/1‑143 (MQ=21)
gCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGTATCAAACCCTTCCATa < 1:167658/143‑1 (MQ=255)
cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑ACATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTACTTTTT‑CAACCTCTTCACACCCAGTCTCAAACCCTTCCAtcc > 2:422046/1‑141 (MQ=18)
cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CG‑CTGCATCCGACATCAATGCCTGATGCGACACTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGTATCAAAcc > 2:107904/1‑134 (MQ=21)
cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CG‑CTGCATCCGACATCAATGCCTGATGCGACACTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGTATCAAAcc < 1:107904/134‑1 (MQ=21)
gTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGTATCAAACCCTTCCATACa > 1:530854/1‑143 (MQ=255)
aGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGGCTGATCAGGCCTATCTTAACCGTTGGTTAGTTTTT‑CAAACTCTTCACACCCGGTATCAAACCCTCCCATCCAAATCAGGTTc > 2:30867/1‑143 (MQ=21)
gTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCaa < 1:72376/143‑1 (MQ=255)
tCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGTATCAAACCCTTACATACAGCTCACGTTCAAc > 2:454461/1‑143 (MQ=25)
tCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGGTTCTCACCCTTCCATACAGCTCAGGTTCacc > 1:686397/1‑141 (MQ=21)
cGGATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTACAAAC‑CATCACGCCCGGTCTCAAACCCTTCCATACAGCTCAGGTTCAAcc > 1:1004265/1‑143 (MQ=21)
ggATAAGGCGTTT‑‑ACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CACACTCTTCACACCCGGTATCAAACCCCTCCCTACAGCTCAGGTTCAACCa > 1:285761/1‑143 (MQ=25)
||
TTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTT‑CAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCA > NC_000913/4296265‑4296507
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
