Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F110 I2 R1
|
16 |
53.0 |
1914758 |
91.2% |
1746259 |
142.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,296,381 |
+GC |
intergenic (+587/+55) |
gltP → / ← yjcO |
glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,296,380 | 1 | . | C | 94.4%
| 93.5
/ 0.1
| 36 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (15/19); total (16/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 |
| * | NC_000913 | 4,296,380 | 2 | . | G | 94.3%
| 94.7
/ 0.1
| 36 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (15/18); total (16/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGT > NC_000913/4296300‑4296509
||
cTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAc < 1:552391/143‑1 (MQ=2)
ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTTTCAGGCCTATCTTAAcc > 1:272602/1‑143 (MQ=255)
aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTCATGCGACGCTTGCCGCGTCTTATCCGGCCTATCTTAACCgcg > 2:154343/1‑141 (MQ=11)
aCGCGTCTTATCAGCCCTCCACCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtt < 1:641694/143‑1 (MQ=11)
cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg < 1:838167/143‑1 (MQ=255)
cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg < 1:224765/143‑1 (MQ=255)
gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGtt > 1:953379/1‑143 (MQ=255)
gTCTTATCAGGCCTACGCCAGACAGCAAAAGATCCTGATTTAGCGTGATCTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCATGCCGCGTCTTATCAGGCCTATCTCAACCGATGGtt > 2:580605/1‑143 (MQ=11)
cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTATGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa < 1:851768/143‑1 (MQ=255)
ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAACGCGTTTA‑‑CGAC‑CCATCCGACATCAATAAATCGCGCTACCCTTGCCGCGCCTTATCAGGCCTATCTTACGCGTTGGTTCAt > 1:489128/1‑143 (MQ=11)
aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTATTTCACATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttt < 1:694380/143‑1 (MQ=14)
tCAGGCCTACGCCTGACAGCGCGATAGCCTGATTCAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTACTC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAtttt < 1:171489/143‑1 (MQ=14)
aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc > 1:445115/1‑143 (MQ=255)
ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc < 2:508844/143‑1 (MQ=255)
aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCacac > 1:203320/1‑143 (MQ=255)
gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGt < 1:954373/143‑1 (MQ=255)
cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTa < 2:647631/143‑1 (MQ=255)
gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 1:331231/143‑1 (MQ=255)
ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTGTTCAAACTCTTCACACCCGGTAGCAAAc > 2:943011/1‑143 (MQ=18)
ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACACTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc > 1:583522/1‑143 (MQ=255)
tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTCACCGTTGGTTAATTTTTCATACTCTTCTCACCCGGTATCAAAccc > 1:451816/1‑143 (MQ=18)
gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCt > 2:348356/1‑143 (MQ=255)
aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt > 2:750716/1‑143 (MQ=255)
ttAGCGTGATTGTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTcc < 1:120646/143‑1 (MQ=255)
gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa < 1:336643/143‑1 (MQ=255)
gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGCTACCAAACCCTTACATa > 1:689075/1‑143 (MQ=18)
tGATTTTGTAGGTCGGATACGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAg < 2:530767/143‑1 (MQ=255)
tGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAg < 2:11999/143‑1 (MQ=255)
tGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAg > 2:69561/1‑143 (MQ=255)
aTTTTGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCCTAACCGTTGGTTACTTTTCCAAACCCTTCACACACGCTATCAAACCCTCCcacacacca > 2:190352/1‑140 (MQ=17)
ttttGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTc < 1:714996/143‑1 (MQ=255)
ttGTAGGTCGGATAAGGCGTTTA‑‑CG‑CTGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAg < 2:1982/143‑1 (MQ=255)
tGTAGGTCGGATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAgg < 1:832869/143‑1 (MQ=255)
ggTCGGAGAAGGCGGTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCa < 1:211406/143‑1 (MQ=25)
ctgATAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAcc < 1:796077/141‑1 (MQ=255)
aTAAGGCGTTTACGC‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGCCTTATCCGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCaat > 1:149029/1‑141 (MQ=37)
||
CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGT > NC_000913/4296300‑4296509
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
