Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 185 118.8 2757226 85.6% 2360185 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C94.2% 275.2 / 6.4 103intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (1/5);  new base C (45/48);  total (49/54)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*NC_0009134,296,3802.G94.2% 276.3 / 5.1 103intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (1/5);  new base G (47/49);  total (49/54)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 103 total aligned reads displayed.

CCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATT  >  NC_000913/4296206‑4296604
                                                                                                                                                                                   ||                                                                                                                                                                                                                                    
ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAc                                                                                                                                                                      <  1:701917/237‑1 (MQ=1)
 cTACGCCAGACAGCGAAAGTGCCTTATTGAGGGTGATTTTGTAGGTCGGAAAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                                                                                                                                                                    <  1:905799/238‑1 (MQ=11)
    cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCACACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTT‑‑ACCC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACCCTATCCGCGTCTTACGAGTCCTATCTTCACCGTTgg                                                                                                                                                                >  1:743137/1‑239 (MQ=11)
         gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCACTAGCCTGATTTAGCGTCATTTTGTAGGTCCGATAAGGCGTTT‑‑AAGC‑‑CGC‑‑ATCTCACATCAATGCTTGATCCGTCACTCGCCCCGCCTTATCAGACCCATCTTAACCGTTgggaaat                                                                                                                                                           >  2:727297/1‑234 (MQ=11)
              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTT‑‑ACGC‑‑CCC‑‑ATCCGACCTCAATGCCCGATGCGCCGCTTGCCGCGCCTTATCTGGCCTATCTTACCCATTGGTTAATTTTTc                                                                                                                                                      >  1:669077/1‑239 (MQ=11)
              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCCG‑‑C‑‑CGC‑‑ATCCGACCTCAATGCCTGATGCGACGCTTGCCGCGTCTTTCAAGGCCTATCTTACCCGTTGGTTACTTTTTc                                                                                                                                                      >  1:154484/1‑239 (MQ=11)
              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc                                                                                                                                                      >  1:110501/1‑239 (MQ=255)
              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CCC‑‑ATCCGACATCAATGCCTGATCCGACGCTTGCCGCGTCTTATCCGGCCTAACTTAACCGTTGGGTAATTTTTc                                                                                                                                                      >  2:1009912/1‑239 (MQ=11)
                 agggacaggATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                                                                                   <  1:428381/231‑1 (MQ=255)
                 aaatgCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                                                                                   <  1:1201061/235‑1 (MQ=255)
                 aaTTGCCTGATTTAGCGTTGTTTTGGAGGGCGGATAAGGCT‑GTTAGGCCGCATC‑CGACATCAACGCCTGAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                                                                                   <  1:1320174/239‑1 (MQ=14)
                  aTAGCCTGATTTAGCTTGATTTTGTAGGACGTGTAAGGCC‑TGTATGCCCACTC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                                                                                   <  1:128302/238‑1 (MQ=14)
                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTATCGTGATTTTGTAGTTCGGATAAGCCGTTT‑‑AAGC‑‑CCC‑‑ATCCGACATCAATGCCTATTGCGACGCTTGCCGCGTCTTTCCAGGCCAACTTTAACCATTGGTTAATTTTTCaacccctcca                                                                                                                                            >  2:164398/1‑231 (MQ=11)
                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                                                                                                                             >  1:1110731/1‑238 (MQ=255)
                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACCCTTc                                                                                                                                             >  1:966677/1‑238 (MQ=255)
                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca                                                                                                                                            <  1:1181387/239‑1 (MQ=255)
                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAATTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGCCCATTGCCGCGTCTTTACAGGCCTATCTTAACCGTTGGTTAATTTTTCaaccctttaa                                                                                                                                            >  2:1374224/1‑231 (MQ=11)
                           tttAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTcaca                                                                                                                                          >  1:304901/1‑238 (MQ=255)
                            tttgcgtttttttGTGGGGGGGATAAGGGT‑TTTACTCCGGCCC‑CGGAATCAAAGCCTGATGCCAAGCTTAACGGGTTTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                        <  2:1355405/230‑1 (MQ=14)
                            ttAGGGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCAGC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCacac                                                                                                                                         <  1:164398/238‑1 (MQ=255)
                            ttAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                        <  1:580367/239‑1 (MQ=255)
                             tacggtttttttGTGGGTCGGA‑GAGGCGTTTTATGCACCAGC‑CGACATCAACGCCTGAAGGGACGCTTAACGGGTCTTATCCGGCCTTAGGCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                                                                                       <  2:378939/231‑1 (MQ=14)
                                cGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTCGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGTGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCTTTGGTTAATTTTTCAACCTCTTATCACCCgga                                                                                                                                    >  2:310663/1‑238 (MQ=14)
                                cGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTCCCGCGTCTTATCAGGCCTATCTTAACCGTAGGTTAATTTTTCAAACTCTTC‑CAccccggc                                                                                                                                   >  2:1221270/1‑235 (MQ=14)
                                    aTTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTTACCGTTGGGTAATTTTTCAAACTCTTCACACCCgcattca                                                                                                                                >  1:324276/1‑233 (MQ=255)
                                    aTTTTGGAGGTCGTATAAGGTA‑TTTATCCCCCATC‑CGACATACACGGCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCa                                                                                                                                <  1:501910/239‑1 (MQ=17)
                                     ttttGGAGGTCGGATAAGGCC‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaa                                                                                                                               <  1:1009912/239‑1 (MQ=255)
                                      ttttttgggtggATAAGGCT‑GTTTGGCCGCATCGCGA‑GTCAACGCCTGAGGCGGCGCTTAACGTGTCTTATCAGGCCGACGACGGACAGCGCAATAGGCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCTTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACGCCCGGTATCaaa                                                                                                                              <  2:743137/229‑1 (MQ=14)
                                      ttttcaGGTGGGATAGGGCG‑TTTATGCCCCATC‑CGACATCAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCTTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGGAGGGCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                              <  2:1363839/234‑1 (MQ=17)
                                      tttGTAGGTCGGATAAGGCG‑TTTATGCCGCAGC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                              <  1:1295294/239‑1 (MQ=255)
                                               cGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCa                                                                                                                      <  1:125638/238‑1 (MQ=255)
                                               cGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGGTTCAAACCCTTCCAt                                                                                                                     >  1:530857/1‑239 (MQ=255)
                                                ggATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                                                                                                                    <  1:893090/239‑1 (MQ=255)
                                                 ggtAAGGCT‑TTTTGGCCGCACC‑CCACATCAACGCCTGGTGCGCCGCTTAACGTGTCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                                                                                                                    <  2:147939/236‑1 (MQ=17)
                                                           tttATGCCGCATC‑CAACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                                                                                          <  1:727297/239‑1 (MQ=255)
                                                            ttATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGGGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGTCGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                                                                                          <  1:1015434/238‑1 (MQ=255)
                                                            ttATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                                                                                          <  2:1073770/238‑1 (MQ=255)
                                                             tATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCCACCCGGTATCAAACCCTTCCATACAGCTCAGGtta                                                                                                        >  2:759594/1‑238 (MQ=255)
                                                             tATGCCGCATC‑CGACATCAACGCCTGAGGCGACGCTTACCGAGTCTTATCAGGCCTACGCCAGACAGCCCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑CTCCGACATCCATGCCTGCTGCGGCCCTTCCCACGCATCATCCGCCCTATCTTAACCGTAGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTCCCATCCACCCCAgatac                                                                                                        >  2:247444/1‑235 (MQ=17)
                                                                gCCCCATC‑CGACATCAACGCCTGAGTCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                                                                                     <  1:663083/239‑1 (MQ=255)
                                                                   gCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCCACCCGGACTCAAACCCTTCCATACAACCCAGGTTCAAcacg                                                                                                  >  2:1286417/1‑236 (MQ=17)
                                                                   gCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAACTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTAAGGGTCAACAAg                                                                                                  >  1:885957/1‑239 (MQ=255)
                                                                    cacc‑cGACATCAACGCCTGATGGGACGTTTAAGGGTTTTTATCAGGCCGACGCCAGACAGCGGAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                               <  2:710139/222‑1 (MQ=18)
                                                                     aTC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCCTCACGCTCAGGTTCAACCAGt                                                                                                 >  1:1290410/1‑238 (MQ=255)
                                                                      tC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTAAGGTTCAACCAGTGc                                                                                               >  1:305306/1‑239 (MQ=255)
                                                                         cgcaaTCAACGCCTGATGCGGCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                                                                             <  1:1342855/235‑1 (MQ=255)
                                                                         ccccctcacccccTGAGGGGACGGTTAGTGCTTCTTATCAGGGCTACGCCAGACGGCGCAATGGCCTAATTTAGCGTGTTTTTCTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGAGATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                                                                             <  2:1103575/228‑1 (MQ=18)
                                                                         cGACATCAACGCCTGATGGGAGCGTTAACGGGTTTTATCAGGCCTGCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGGAGGGCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                                                                             <  2:110501/239‑1 (MQ=18)
                                                                              ataaCGGCTGATGGGAACCCTAACGTGTCTTATCAGGCTTAAGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCCGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCTACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                                                                                        <  1:734946/237‑1 (MQ=21)
                                                                               cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCATTGCCTGATGCGACGCTTGCCTCGTCTTATAAGCCCTACCTTAACCGTTGGTTAATTTTTCACACTCTTACCACCCGGATTCACACCCCTCCACAAAACCCAGGATAAACACATGCAGCGCCtt                                                                                       >  2:354614/1‑239 (MQ=14)
                                                                               cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACCTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTAAAc                                                                                                     >  1:710139/1‑225 (MQ=255)
                                                                                  cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca                                                                                                                                            >  1:1185027/1‑183 (MQ=255)
                                                                                  cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATAAGGCCTATCTTAACCGTTGGTTAATTTTTAAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCACAGGTTCACCCAGTGCCGCGCCttt                                                                                      >  1:1286825/1‑237 (MQ=18)
                                                                                  cGCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTcc                                                                                                                                            <  2:1185027/183‑2 (MQ=255)
                                                                                   gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTTCGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCTTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCGGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCTTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGc                                                                                   <  1:146140/239‑1 (MQ=21)
                                                                                       gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGt                                                                               >  1:397598/1‑239 (MQ=255)
                                                                                         tGCGACGCTTAACGCGTCTTATCAGGCCGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttc                                                                             <  1:5393/239‑1 (MQ=255)
                                                                                           ccaggtttAACTGGTTTTATCAGGCCTACGCCAGGCAGGGTAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGACAAGGCGTTT‑‑ACGC‑‑CGC‑‑AGCCGACATCAATGCATGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttctt                                                                           <  2:1042113/233‑1 (MQ=21)
                                                                                            ggcgtttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttctt                                                                           <  1:1323331/233‑1 (MQ=255)
                                                                                            gaagtttAACGCGTCTTATCAGGCCTACGTCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttctt                                                                           <  1:1307546/233‑1 (MQ=255)
                                                                                             aCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ATGC‑‑CGC‑‑ATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTATCTTAACCGTTGGGTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGtccttc                                                                          >  1:937306/1‑238 (MQ=18)
                                                                                             aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttcttc                                                                          >  1:1302305/1‑238 (MQ=255)
                                                                                               gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGCCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCCACCCGGTATCAAACCCTTCCTTACACCCCAGGTTCAACCAGTCCAACCCCTTTTCCTTGtctttcccg                                                                       >  2:707300/1‑231 (MQ=18)
                                                                                                    acggtttttttCAGGCCTACGCCAAACAGCGCAATACCCTTATTTAGGGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaa                                                                  <  2:1367282/229‑1 (MQ=25)
                                                                                                    accgggtttATCCGGTCTACGCCAGACAGCGCAATAGCTTGATTTAGCGTGATTTGTTAGGTCGGATAAGGCGGTT‑‑ACGC‑‑CGC‑‑AACCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTAGCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaa                                                                  <  1:1374224/232‑1 (MQ=25)
                                                                                                     cgcgTTCTATCCGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaaa                                                                 <  1:782491/239‑1 (MQ=255)
                                                                                                         tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                              >  2:218522/1‑174 (MQ=255)
                                                                                                         tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                              <  1:218522/174‑1 (MQ=255)
                                                                                                          cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACct                                                              >  1:436978/1‑237 (MQ=255)
                                                                                                          cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGAGCAGCGCCTTTTGCTTGTTCTTTTCGATAAAACct                                                              >  1:30161/1‑237 (MQ=255)
                                                                                                           ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGCCATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTAACACCCGGTATCAAACCCTTCCATACAGCTATGGTTCAACCAGTGAATAGCCTTTTGCTTGTTCTTCTCGAGAAAACcttct                                                           >  1:834399/1‑239 (MQ=21)
                                                                                                           ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCCTACAGCTACGGTTCACCCAGAGCAGCGCCTTTTGCTCTTTCTTCTCGATAAAAAcctct                                                           >  2:1240585/1‑239 (MQ=21)
                                                                                                              tagggCCTGCGCCAGACAGCGGAATGACCTGATTTAGCGGTATGTGTGAGGTGCGATTAGGCGTTT‑‑AAGG‑‑CGG‑‑ATCCGACATCAATGCCGTATGCGACGCTTGCCGCGTCTTATCAGGCCTAGCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCa                                                        <  2:154484/236‑1 (MQ=25)
                                                                                                               aagcaagcggCCAGGCAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGGCATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAGCCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAc                                                       <  1:1238115/230‑1 (MQ=25)
                                                                                                                  gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCACCCAATGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCATCTTCAccct                                                    >  2:560498/1‑237 (MQ=255)
                                                                                                                       cgcacgccAGCGCTATAGCCTTGTTTAGCGGGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTGCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                               <  2:397598/232‑1 (MQ=37)
                                                                                                                       ccccagccagTGCATAAGCCTGATTTAGCTTTATTTTTTAGGTCGGGTAAGGCTTTTCCG‑‑C‑‑CGG‑‑ATCCGAAATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                               <  2:65001/237‑1 (MQ=37)
                                                                                                                       cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                                                                                     >  2:761427/1‑185 (MQ=255)
                                                                                                                       cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                                                                                     <  1:761427/185‑1 (MQ=255)
                                                                                                                       cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTaaa                                               >  2:532490/1‑238 (MQ=255)
                                                                                                                       cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGAAGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTAACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAATGCAGCGCCTTTTTCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTaaa                                               >  2:1029996/1‑238 (MQ=255)
                                                                                                                            gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGAATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGTTAAAACCTTCTTCACCGTTTAAGAAca                                           >  1:1241205/1‑238 (MQ=255)
                                                                                                                                 cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGCTCACCCAGTCCAGCGCCTTTTGCTTGTTTTTCTCGCTAAAACCTTCTCACCCGTTTAAGAActtgcctt                                     >  2:1238115/1‑232 (MQ=25)
                                                                                                                                 cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTACCAAACCCTTCCATAAAGTCCAGGTTCAACCAGTCCAGCGCCTTTTGCTTGTTTTTCTCTTTAAAACATTCTTCACCGTTAAAAAAcaacttt                                      >  2:1059770/1‑233 (MQ=21)
                                                                                                                                   cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTccc                                   <  1:1221270/239‑1 (MQ=255)
                                                                                                                                      tAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCTTTT‑‑A‑‑CGACGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                <  1:354614/239‑1 (MQ=255)
                                                                                                                                      tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                <  1:530715/239‑1 (MQ=255)
                                                                                                                                      tAGCCTGATTTAGCGTGATTTTGGAGGTCGGATAAGGCTTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                <  1:1286417/239‑1 (MQ=255)
                                                                                                                                      attgctcttttAGCGTTGTTTTGTAGGTGGGTGAAGGGGTGT‑‑A‑‑CGTCGC‑‑CACCGACATCAAGGCCCGGTCGGACGCTTAGCGGATCTTATTAGGCCAATCTTTACCGTTGGTTAATGTTTCAAACTCTTCACACCCGGTATCAATCCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGGTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                <  2:669077/231‑1 (MQ=255)
                                                                                                                                           tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCCTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTa                           <  1:1301753/239‑1 (MQ=255)
                                                                                                                                                   cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATTCGACATCAATGCCTAGTGCGCCGCTTGCCGCGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACACTTCCCTAAAGCACAGTATAAACCCAGGCATCGCCTTTTGCTTGTTCTTATCGATACAACCTTCTTCACAGTTTAGGAACATAATTCCCACCCAgaatcgtgata                   >  2:898143/1‑229 (MQ=21)
                                                                                                                                                   cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGTTAAACCCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGata                   >  1:688749/1‑237 (MQ=255)
                                                                                                                                                   cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAACCCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAAt                   >  1:809795/1‑239 (MQ=255)
                                                                                                                                                   cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGAACCCGGaaa                   >  2:28726/1‑238 (MQ=255)
                                                                                                                                                       aTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                        >  1:800651/1‑118 (MQ=255)
                                                                                                                                                       aTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                        <  2:800651/118‑1 (MQ=255)
                                                                                                                                                           tttAGGTCGGATAAGGGGTTTCCC‑‑C‑‑CGC‑‑ACCCGACAGCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGtt           <  2:848491/237‑1 (MQ=39)
                                                                                                                                                             ttggtgcgttAAGGCGTTT‑‑A‑‑C‑‑GGCCAATCCAGCATCAATGCCGTCTGCCACGCTTGCCCCGTTTTATCAGGCCATGCTTAACCGTTGGTTAATTTTTCAAACGCGTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCg         <  2:304901/230‑1 (MQ=255)
                                                                                                                                                                 tCGGATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa      <  1:985433/238‑1 (MQ=255)
                                                                                                                                                                    gATAAGGCGTTT‑‑ACGC‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCATCCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAAAACATCATTCCCGCCCATAAACCGAAATAACCGTTCCGGGaaaat  >  2:395447/1‑237 (MQ=38)
                                                                                                                                                                                   ||                                                                                                                                                                                                                                    
CCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑TTTATGCCGCATC‑CGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CGC‑‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATT  >  NC_000913/4296206‑4296604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A