Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 185 118.8 2757226 85.6% 2360185 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,319,404 A→C 19.4% V68G (GTG→GGG)  phnJ ← carbon‑phosphorus lyase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,319,4040AC19.4% 219.5 / 7.1 111V68G (GTG→GGG) phnJcarbon‑phosphorus lyase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (48/36);  new base C (4/23);  total (52/59)
Fisher's exact test for biased strand distribution p-value = 1.22e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGT  >  NC_000913/4319168‑4319635
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          
gcgcGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGGGGTGTCATCCGCGCCCTGGTCAATCa                                                                                                                                                                                                                                            >  1:226225/1‑237 (MQ=255)
     tGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGGTGCGGGGACGCGTCTGGATAACCGTCGCATCGTCCGTCCGTTCCGTGGGGTTTACCCCGGTCACGCCCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCCTCCGCCCCCCGGTCAATCACCTTCa                                                                                                                                                                                                                                      >  2:819076/1‑238 (MQ=255)
     tGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGCCATCGGCGCCCTGGTCAATACCCTTCa                                                                                                                                                                                                                                      >  1:246565/1‑238 (MQ=255)
     tGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGGGTCATCCGCGCCCTGGTCAATCACCTCCa                                                                                                                                                                                                                                      >  1:1061545/1‑238 (MQ=255)
     tGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGGGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACCCGGCGTTGGGGGTTTCATCCGCGCCCTGGTCAATCACCttaa                                                                                                                                                                                                                                      >  1:8008/1‑236 (MQ=255)
     tGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGGGTTTACCCCGGGCACGCGCTTGAAGAAGTGCCGAATCGCCACGGCGTTGGGGGTGTCATCCCCGCCCTGGTCAATCACCttta                                                                                                                                                                                                                                      >  2:1271042/1‑236 (MQ=255)
     tGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGGGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAGTCGCCACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCa                                                                                                                                                                                                                                      >  1:1357600/1‑238 (MQ=255)
          ggTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACg                                                                                                                                                                                                                                 <  1:1248744/238‑1 (MQ=255)
           gTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGGGTTTACCCCGGTCACGCGCTTGAAGCAGTTGCGAATCGCAACGGCGTTGGTGGGGGCACCCGGCCCCGGGTCAATCACCCTccgaaaccc                                                                                                                                                                                                                               >  2:29549/1‑231 (MQ=255)
            tGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGtg                                                                                                                                                                                                                               >  1:859355/1‑237 (MQ=255)
               gggTTTCCGTTTCGCGCGGCTCGATTAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCt                                                                                                                                                                                                                            <  1:1211485/238‑1 (MQ=255)
                    tCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGTCACCTGGCAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGCTGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTTCGTTCCGTGGGTTTTACCCCGGCCACGCGCTTGAAGAGGTTTGGAATCGACACAGCGTTGGCGGGGTCCTCCGCCCCCGGGTGAATCCCCTTCAACCCGCCCCTTTCGc                                                                                                                                                                                                                       >  2:213753/1‑238 (MQ=255)
                           cgcgcgGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGGTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCAc                                                                                                                                                                                                                >  1:713103/1‑238 (MQ=255)
                            gcgggGCTCGATAAAGGGCCGGGGCTTGGGGATTGGGCCCTGGAAGATAATTATCTGAGCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGc                                                                                                                                                                                                              <  2:1061545/239‑1 (MQ=255)
                             cgcgGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCAc                                                                                                                                                                                                                >  1:966531/1‑236 (MQ=255)
                                          aGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAACCTGa                                                                                                                                                                                                >  1:128674/1‑239 (MQ=255)
                                            cgcAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGGGGTGTCATCCGCGCCCTGGTCAATCCCCTTCAGCACGTCGCTTTCGCCACTCACGCTGGCGGTAAGcgtca                                                                                                                                                                                               >  1:625655/1‑234 (MQ=255)
                                            cgcAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGGGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCCCCAATCACGCTGGCGGTAAGCTGaac                                                                                                                                                                                              >  1:860020/1‑238 (MQ=255)
                                                 cGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGCCACGGCGTTGGTGGGGTCATCCGCGCCCTGGTCAATCACCTTCAGAACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATgccc                                                                                                                                                                                          >  1:684986/1‑237 (MQ=255)
                                                 cGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCCCGACGCTTTCGCCAATCACGCTGGCGGTAAGCTGaaatccg                                                                                                                                                                                          >  1:1207029/1‑233 (MQ=255)
                                                 cGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGGGGTGTCATCCGCGCCCCGGTCAAGCACCTTCCGAACGTCGCTTTCGCCAATCACGCTGGCGGGAAAAGGAATgcccc                                                                                                                                                                                         >  2:549108/1‑237 (MQ=255)
                                                 cGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGGGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGGGGGGTAATCCGCGCCCTGGCCAATCACCTTCCGACCGTCCCTTTTGCCAATCACCCTGCGGGCAAGCGAAATggccc                                                                                                                                                                                         >  2:952635/1‑235 (MQ=255)
                                                  ggttCGGGGTTTGGCCGCTGGAAGATAATTATCTGATCTTCGGTACACGGCGTTTCGGGGGTGCGGGGGCGCGTGTGGATAACCGTCGCCTCGTCCGTACGTTCCGTGGTGTTTACCCCGGTTACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGGGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATgccgcc                                                                                                                                                                                        <  2:860020/236‑1 (MQ=255)
                                                    cTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATgcc                                                                                                                                                                                           <  1:1105581/234‑1 (MQ=255)
                                                     tCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCgg                                                                                                                                                                                      <  1:1098012/238‑1 (MQ=255)
                                                          gATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTccc                                                                                                                                                                                 <  1:819076/238‑1 (MQ=255)
                                                              ggCCCCTGGAAGCTAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCCTCCCCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCCCCACTCCCGCCGGCGGTAAGCTGAAAGCCGCCGGGTCCCCAGcc                                                                                                                                                                            >  2:8119/1‑239 (MQ=255)
                                                              ggCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCCCCAACCACCCTGGCGGGAAGCTGGATgccgcccggaccccccg                                                                                                                                                                             >  2:1105581/1‑227 (MQ=255)
                                                                   cTGGAAAATAAATTTCTGATCTTCGGGCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTAcg                                                                                                                                                                       <  1:837127/239‑1 (MQ=255)
                                                                                 tCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGGCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCCGCCGGCAAACCGAATGCCGCCGGTTCCCCAGCCTAAcg‑gcctcggcagaccg                                                                                                                                                        >  2:354409/1‑234 (MQ=255)
                                                                                     aTCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAACCTGAATGCCGCCGGGTCCCCAGCCGTAcg‑gcatcggc                                                                                                                                                              >  1:828608/1‑229 (MQ=255)
                                                                                     aTCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGAACGCCGCTTTCGCCAATACCGCTGGCGGGAAGCTGAATGCCCGCGGGTCCCCCGCCGCAcg‑acatcggcaaccccgcgc                                                                                                                                                    >  2:601769/1‑230 (MQ=255)
                                                                                             tCAGCGGCGTTTGGGGGATGCGGGGACGCGTCTGGATAACCGGCGCATCGTTCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCaaa                                                                                                                                             <  1:237890/238‑1 (MQ=255)
                                                                                               aGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGGCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACgg                                                                                                                                          <  1:29549/239‑1 (MQ=255)
                                                                                               aGCGGCGGTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACgg                                                                                                                                          <  1:402930/239‑1 (MQ=255)
                                                                                                 cggcgTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCCCCGGTTCCCCCGCCGTCCG‑GCATCGGCCTCCCGCGCCCCCCAAACGGc                                                                                                                                         >  2:983550/1‑238 (MQ=255)
                                                                                                  ggcgTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACgg                                                                                                                                          <  1:213753/236‑1 (MQ=255)
                                                                                                     gTTTGGGGGTTGGGGTGACGCTTCTGGATACCCGTCGCATCGGCCGTCCGTTCCGTGGTGTTTCCCCCGGCCACGCGCTTGAAGAAGTTGGGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCCCCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCt                                                                                                                                     <  2:713103/238‑1 (MQ=255)
                                                                                                      tttCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCCGCCGTACG‑GCATCGGCATACCGCGCCCGCCaacaggccacccg                                                                                                                                   >  2:39195/1‑228 (MQ=255)
                                                                                                               tGCGGTGACGCTTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTGCCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCggg                                                                                                                           <  1:1305630/238‑1 (MQ=255)
                                                                                                                      gcccctttGGATAACCGGTGCATGGTGCGTAGGTTCCGTGGTGTTTTCCCCGGGCCCGCGCTTGAAGAAGTTGGCAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCCCCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCa                                                                                                                  <  2:440593/232‑1 (MQ=255)
                                                                                                                       gggggctggtcaaCCTTCGCTTCGGCCGTCCGGTCCGGGGTGTTTCCCCCGGTCACGCGCTTGAAGAAGTTGCGAACCGACACGGCGTTGGTGGGGGCATCCGCGCCCTGGTCAATCACCTGCAGCACGTCGCTTGCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCa                                                                                                                  <  2:1275872/227‑1 (MQ=255)
                                                                                                                       cggggttGGATAACCGTGCCAACGTCCGTACGGTCCGGGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCa                                                                                                                  <  1:716106/232‑1 (MQ=255)
                                                                                                                        gcgttgggATAACCGTCGCAGCGTCCGTACGTTGCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTTTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCAc                                                                                                                 <  1:885873/232‑1 (MQ=255)
                                                                                                                        gcgTCTTTATATCCGTGGCGTCGTCGGTCGGTTCCGGTGTGTTTCCCCCGGTCACGCCGTTGAAGAAGTTGCGAATCGACCCGGCTTTGGTGGTGTCACCCGGGCCCTGGTCAATCCCCTTCAGCACGTCGCTTCCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCAc                                                                                                                 <  2:68998/238‑1 (MQ=255)
                                                                                                                                     cGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGCTAACCGGGGATCC‑CCCCCGCTTTTAAAATgg                                                                                                   >  1:1256587/1‑239 (MQ=255)
                                                                                                                                     cGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAACTGAATGCCCCCGGGTCCCCAGCCTGACGCGCA‑CGGCCTTCCGCGCCCCCCCAACGGCCCCGCAAAACCCGGGAACGCCCACCCCTTTaaacggg                                                                                                    >  2:658094/1‑235 (MQ=255)
                                                                                                                                      gTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGGACCTGATAACCGGGGATCG‑CCACCGCtgtcccttttac                                                                                                  >  1:295339/1‑228 (MQ=255)
                                                                                                                                        cGCATCGGCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGgcgc                                                                                                <  1:439859/239‑1 (MQ=255)
                                                                                                                                         gCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCCCGCCCGCCCAACGGCACCTGATAACCGGGGCTCT‑CCACCGCCTTTAACATGGcccg                                                                                               >  1:516455/1‑239 (MQ=255)
                                                                                                                                           agcggccGTAAGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCgcgg                                                                                              <  1:845061/233‑1 (MQ=255)
                                                                                                                                               tCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCCGCATCTCCCGCCCGCCAAACGGGACCTGTTAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGcggcggc                                                                                          >  1:875487/1‑237 (MQ=255)
                                                                                                                                                ccGTACGTTCCGTGGTTTTTCCCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑At                                                                                        <  1:1148107/238‑1 (MQ=255)
                                                                                                                                                ccGTACGTTCCGGGGGTTTTTCCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACCCGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCCCCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTGAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑At                                                                                        <  1:1227957/238‑1 (MQ=255)
                                                                                                                                                 cGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCa                                                                                      <  1:39195/239‑1 (MQ=255)
                                                                                                                                                    aCGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCCTCGGCATCTCGCGCCCGCCAAACGGACCCTGCTAACCGGGGATCG‑CCACCGCTTTTAAAATGGCGCGGCGG‑ATCATGc                                                                                   >  1:86318/1‑239 (MQ=255)
                                                                                                                                                         ccGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCCGATAACCGGGGATCG‑CCACCCCTTTTAAGATGGCGCGCGGG‑ATCAAGCGTTTg                                                                              >  1:66753/1‑239 (MQ=255)
                                                                                                                                                         ccGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCCCCAAACGGCACCTGATAACCGGGGATCG‑CCCCCGCTTTTAAGATGGCCCGGCGG‑CTAATGCGTTTg                                                                              >  1:306510/1‑239 (MQ=255)
                                                                                                                                                          cGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAAATGGCCCGGCGG‑GCCATGCGTTTgg                                                                             >  1:473196/1‑239 (MQ=255)
                                                                                                                                                              gtttttACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACCCGGCGTTGGTGGTTGCATCCGCGCCCTGGTCAATCCCCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTg                                                                         <  1:658094/236‑1 (MQ=255)
                                                                                                                                                               tgtTTACCCCGGTCACGCGTTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCCCCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTg                                                                         <  1:8119/238‑1 (MQ=255)
                                                                                                                                                                 tttACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGCCACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTCAACTGAATGCCGCCGGTGCCCCAGCCGTACG‑GCATAGGCATATCGCGCCCGCCCAAGAGGACCTGATAACCGGGGGACG‑CCCCCCCTGTTTATGGGGCGCGGGGGCGTCA‑GCGTTTGGTCTGCt                                                                       >  2:926941/1‑238 (MQ=255)
                                                                                                                                                                 tttACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGGAAGCTGAATGCCGCCGGTTCCCCCGCCCTACG‑GGCACGCCATATCCCGCCCGCCCAACGGGCCCCGCTAACCGCGGGGCC‑CCCCCCCCTTTCAGAGGGCGCGGCGG‑ACCAACCGTTTGGCGTGCtc                                                                      >  2:207616/1‑239 (MQ=255)
                                                                                                                                                                   tACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTgg                                                                                                                                                                                                           >  1:605264/1‑107 (MQ=255)
                                                                                                                                                                   tACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTgg                                                                                                                                                                                                           <  2:605264/107‑1 (MQ=255)
                                                                                                                                                                       ccgtgcccgtccTTGAAGAAGTTGGTAATCGACGCGGCGTTGGTGGGGTCATCCGCGCCCGGGTCAACCCCCTTCACCCCGCCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAg                                                                <  2:966531/228‑1 (MQ=255)
                                                                                                                                                                       ccGGTAACGGGCTGGAAGAAGTTGGGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCCCCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAg                                                                <  1:531693/239‑1 (MQ=255)
                                                                                                                                                                         ggTCACGCGCTTGAAGAAGTTGCGAATCGACTCGGCGTTGGTGGTTTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCCCGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGt                                                              <  1:991884/239‑1 (MQ=255)
                                                                                                                                                                         ggTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCCTCGGCATCTCGCGCCCGCCCAACGGCACCTGATAAACGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑CTCCTGCGTTTGGGCTGCTCGGCggggg                                                              >  2:129945/1‑238 (MQ=255)
                                                                                                                                                                         ggTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCCCCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCCCGCCGAGGt                                                              >  1:436072/1‑239 (MQ=255)
                                                                                                                                                                             aCGCGCTTGAAGAAGTTGCGAATCGACCCGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCCCCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGc                                                          <  1:980928/239‑1 (MQ=255)
                                                                                                                                                                              cgggcTTGAAGAAGTTGCGAACCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCa                                                         <  1:176130/236‑1 (MQ=255)
                                                                                                                                                                                    tGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCCCCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCCAACGGCACCTGCTAACCGGGGCTCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑CTCATGCGTTTGGGCTGCTCGTCGCGGGTGGCACAGGTg                                                   >  1:187560/1‑239 (MQ=255)
                                                                                                                                                                                    tGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGGTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCCTTTAAGAAGGCCCGGCGG‑ACCCTGGGTTTGGTCTGCCCGTCGGGGTAGGCCAAGTTg                                                   >  1:1167956/1‑239 (MQ=255)
                                                                                                                                                                                     gaagaaGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAACTGAATGCCGCCGGTTCCCCACCCCGCCG‑CCACCCGCACCTCCCGCCCGCCAAACGGACCCTGCAAACCGGGGATCG‑CCACCCCCTTTAAGATGGCGCGGCGG‑CACATGCGTTTTGCTTGCCCGTCGAGGTAGGGaaaaggtg                                                  >  2:567771/1‑234 (MQ=255)
                                                                                                                                                                                       agaatTTGCGAATCGACACGGGCTTGGTGGTGTCATCCGCGCCCTGGTCAATCCCCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAg                                                <  1:1345456/239‑1 (MQ=255)
                                                                                                                                                                                       agaagTTGCGAATCGACACGGCGTTGGTGGTGTCCTCCGGCCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAg                                                <  1:130314/239‑1 (MQ=255)
                                                                                                                                                                                       aaatgtggggAATCGCCGCGGGTTTGGGGGGGTCACCCGCGCCCTGGTCAATCCCCTGCAGCCGGTCGTTTTCGCCAACCACGCTGGCGGTAAGCGGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAg                                                <  2:859355/230‑1 (MQ=255)
                                                                                                                                                                                          agTTGCAAATCGACGCGGCGTTGGGTGTGTCCTCCGCGCCCGGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGc                                               <  1:772967/237‑1 (MQ=255)
                                                                                                                                                                                            ttgcgattgtgcacGGGGTTGGTGGTGTCACCCGGGCCCTGGTAAACCCTCTTCAGCACGTCGCTTTCGCCCATCCGGCTGGCGGTAAGCTGAATGGCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCGCGCGCCCGCCAAACGGCACCTGATAACCGGTGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCt                                           <  2:246565/228‑1 (MQ=255)
                                                                                                                                                                                             tGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCCGCCGTACG‑GCATAGGCATCCCGCGCCCGCCAAACGGCACCTGGTAACCGGGGAACG‑CCCACGCTCTTAAACTGGCCCGGCGG‑AACATGCGTTTGGTCTGCCCCCCGGGGGAGGCAAAGTTgggggcgccg                                          >  2:718775/1‑230 (MQ=255)
                                                                                                                                                                                             tGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCt                                           <  1:207616/238‑1 (MQ=255)
                                                                                                                                                                                             tGCGAAGCGACACGGGGTTGGTGGTGTCATCCGCGGCCTGGTCAATCACCTTCAGCACGTCCGTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCt                                           <  1:991156/238‑1 (MQ=255)
                                                                                                                                                                                               cGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGCTCCCCAGCCGTACG‑GCATTGGCATCTCGCGCCCGCCAAACGGCACCTGTTAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCCTGAGATTTGTTTGCTCGGCGAGGGTGGCAAAGTTGTAGACGCTccg                                        >  1:407660/1‑237 (MQ=255)
                                                                                                                                                                                                gattCGACCCGGTGTTGGTGGTGTCACCCCCGCCCTGGTCAGTCCCCTTCAGCACGTCGCTTTCGCCATCCACGCTGGCGGTAAGCTGAAGGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCCCCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGa                                       <  2:436072/236‑1 (MQ=255)
                                                                                                                                                                                                 aaTCGTCACGGCGTTGGTGGTGTCATCCGCGGCCTGGTCAATCCCCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGa                                       <  1:211670/238‑1 (MQ=255)
                                                                                                                                                                                                 aaTCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGCGGACGGCAAAGTTGTAGCCGCCCAAAt                                      >  1:878664/1‑239 (MQ=255)
                                                                                                                                                                                                       cccGGCGTTTGTGGTGTCCTCCCCGCCCGGGTCAATCACCTTCACCCCGTCGGTTCCGCCAATCCCGCGGGCGATAAGCTTAATGCCGCCGGTCCCCCAGCCTTACG‑GCATCGGCATCCCGCTCCCGCCAAACGGCACCTGATAACCGGGGATCT‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCa                                <  2:1167956/237‑1 (MQ=255)
                                                                                                                                                                                                       cccGGCGTTGGGGTTTTCCTCCCCGCCCTGTTCAACCCCCTTCCGCCCGTCGCTTGCGCCAACCCCGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCa                                <  2:1256587/237‑1 (MQ=255)
                                                                                                                                                                                                                  tggttccctCCGCGCCCTGGTCAATCCCCTTCAGCACGTCGCTTTCGCCAATCCCGCTGGCGGTAAGCTTAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCt                      <  1:952635/230‑1 (MQ=255)
                                                                                                                                                                                                                      gtCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGGAGGCAAAGTTGTAGCCGCCCAGATTAGCCATGGTTCTGCTccctt                 >  1:996097/1‑236 (MQ=255)
                                                                                                                                                                                                                          tCCGCGCCCTGGGCAATCCCCTTCAGCCCGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCa             <  1:567771/239‑1 (MQ=255)
                                                                                                                                                                                                                          cccGCGCCCTGGTCAATCCCCTTCAGCACGTCCCTTTCGCCAATCCCGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCa             <  2:684986/238‑1 (MQ=255)
                                                                                                                                                                                                                              cgcCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCAc                                                                                                                 >  2:330095/1‑136 (MQ=255)
                                                                                                                                                                                                                              cgcCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCAc                                                                                                                 <  1:330095/136‑1 (MQ=255)
                                                                                                                                                                                                                               gcCCTGGTCAATCCCCTTCAGCCCGTCGCTTCCGCCAATCCCGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGt        <  1:129945/239‑1 (MQ=255)
                                                                                                                                                                                                                                   tGGTCAACCCCCTTCCCCCCGTCCCTTGCGCCATTCCCGCTGGCGGTAAGCTGAATGCCGCTGGTTCCCCAGCCGTCCG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGa    <  2:778352/239‑1 (MQ=255)
                                                                                                                                                                                                                                    gttCAAGCACCTTCAGCACGGCGCTTTCGCCAATCACGCTGGCCGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAg   <  1:549108/237‑1 (MQ=255)
                                                                                                                                                                                                                                     ttccaTCACCTTCCGCCCGTCGTTTTCGCCAATCACGGTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTCCG‑GCCTCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGt  <  2:128674/235‑1 (MQ=255)
                                                                                                                                                                                                                                      tCAATCCCCTTCAGCCCGTCCCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGt  <  1:1118232/238‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          
GCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACG‑GCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCG‑CCACCGCTTTTAAGATGGCGCGGCGG‑ATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGT  >  NC_000913/4319168‑4319635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A