Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F18 I0 R1 150 105.7 2655328 79.3% 2105675 226.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,319,404 A→C 11.7% V68G (GTG→GGG)  phnJ ← carbon‑phosphorus lyase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,319,4040AC11.7% 210.5 / 2.3 88V68G (GTG→GGG) phnJcarbon‑phosphorus lyase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (44/29);  new base C (3/11);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 9.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGT  >  NC_000913/4319168‑4319635
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          
gcgcGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCCCATCGTCCGTACGTTCCGTGGGGTTTACCCCGGCCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGGGTCTTCCGCG‑CCCCGGGCAACCAcg                                                                                                                                                                                                                                          >  1:240038/1‑238 (MQ=255)
   cgTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCtt                                                                                                                                                                                                                                        <  1:1007101/238‑1 (MQ=255)
   cgTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGGGACGCGTCTGGATAACCGTCGCATCGTCCGTACGGTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGACGTTGCGAAACGCACCGCCGTTGGTGGTGGCA‑CCGCGCCCCCGGCCAATCCCCTTc                                                                                                                                                                                                                                       >  2:1117387/1‑239 (MQ=255)
     tGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGTG‑CCCTGGTCAATCACCTTCa                                                                                                                                                                                                                                      >  1:780587/1‑238 (MQ=255)
     tGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGGCATCCGCG‑CCCTGGTCAATAACCTTcg                                                                                                                                                                                                                                      >  1:263968/1‑237 (MQ=255)
     tGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGAATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTTAAGAACTTTCGAATCGACCAGGCGTTGGTGGGGTCATCCGCG‑CCCTGGCAAATCCCCTTcc                                                                                                                                                                                                                                      >  2:626762/1‑237 (MQ=255)
                       gTTGCGCGCGGGTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAAt                                                                                                                                                                                                                  <  1:690656/239‑1 (MQ=255)
                         tttcggcgGTTCGTTAAATCGCAGCGGCCCGGGGTTTGGCACCTGGGAGATAATTTTCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTCCCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCa                                                                                                                                                                                                                <  2:555193/234‑1 (MQ=255)
                            ggggggCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGGGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGc                                                                                                                                                                                                             <  1:352273/235‑1 (MQ=255)
                                            cgcAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGCC‑GGTAACCTGCAt                                                                                                                                                                                            >  1:1067267/1‑239 (MQ=255)
                                               aGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGGTGGTGGTGTCATCCGCG‑CCCTGGGCAATCAACTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTGAGCTGAATg‑c                                                                                                                                                                                         >  1:1302454/1‑238 (MQ=255)
                                                   gCTGGGGGTTTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGGCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATg‑ccgcc                                                                                                                                                                                     <  1:1117387/238‑1 (MQ=255)
                                                              ggCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGCCATCCGCG‑CCCTGGTCAATCACCTTAAGCA‑CGCCGCTTTCCCCAACCACGCGGGC‑GGGAAGCCGAAAT‑CCCCCCGCTCCCCacc                                                                                                                                                                          >  2:49113/1‑236 (MQ=255)
                                                              ggCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGGCCGTACGTTCCGTGGTGTTTCTCCCGGTCACGCGCTTGAAGCCGTTGCGAATCGACCCGGCGTTGGTGGTGTCATCCGCC‑CCCTGGGCATTCCCCTTCCGCA‑CGTCGCTTTTCCCCACCCCGCACGG‑GGTAACCGGCAGG‑CCGCCGGTGcccccccg                                                                                                                                                                         >  2:1212690/1‑235 (MQ=255)
                                                                  ccTGGGAGATTTTTAGCTGGCCTTCGGGCCGCGGTGTTGCGGGGGTGCGGTGACCCGTCTTGGTAACCGTTGCATGGTCCGTGCGTTCCGTGGTGTTTTCCCCTGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTa                                                                                                                                                                      <  2:263968/238‑1 (MQ=255)
                                                                   cTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGCAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGGAACCTGAATG‑CCCCCGGTTCCCCAGcctgcc                                                                                                                                                                     >  1:515543/1‑234 (MQ=255)
                                                                     ggAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAACGTGAATT‑CCCCCCGTTCCCCACCCggaacg                                                                                                                                                                   >  1:1237394/1‑233 (MQ=255)
                                                                                    gATCTTCGGTCAGCGGCGTTTCGGGGATCCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGTGGGTTACCCCGGTCCCGCGCTTTAAGAAGTTGCGAATCGACACGGAGTTGGTGGTGTCATTCGCG‑CGCTGGTCAATCACCTCCAGCC‑AGTCGCTTTAGCCAATCACGCGGGC‑GGAAACGTGAATA‑CCGCAGGTTCCCACGCCGGAaggcgtaggccgttcgccc                                                                                                                                                   >  2:1073545/1‑230 (MQ=255)
                                                                                     aTCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGGTCCCCAACCGTACGGCATCGGCATCTCgcgc                                                                                                                                                   >  1:205380/1‑238 (MQ=255)
                                                                                     aTCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGCC‑GGGAAGCTGAATT‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCgcgc                                                                                                                                                   >  1:602089/1‑238 (MQ=255)
                                                                                         tCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCACCCGTAcggcatccgcattcccccccccc                                                                                                                                               >  1:700568/1‑227 (MQ=255)
                                                                                             ccAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCaaa                                                                                                                                           <  1:626762/237‑1 (MQ=255)
                                                                                               aGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTCCGGCATCGGCATCCCCCGCCCGCCAAACg                                                                                                                                         >  1:484960/1‑238 (MQ=255)
                                                                                               aGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCCCCCAACg                                                                                                                                         >  1:1319427/1‑238 (MQ=255)
                                                                                                 cggcgTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCTTACGGCATCGGCATCTCGCGCCCGCCAAACGGc                                                                                                                                       >  1:955305/1‑238 (MQ=255)
                                                                                                 cggcgTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGGTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGGTGCGAATCGACCCGGCGTTGGTGTTGTCCTCCGCG‑CGCTGGTCAATCACCTTCATCC‑CGTCGCTTTCGCCACTCACGCTCGC‑GGTCACCGTAGTGCCCGCCGTTTCCCCACCCG‑CCGGCAGCGGGACTCCGCCCCCACCCAACGGc                                                                                                                                       >  2:129059/1‑238 (MQ=255)
                                                                                                  ggcgTTTCGGGGATTCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACgg                                                                                                                                        <  1:49113/236‑1 (MQ=255)
                                                                                                  ggcgTTTCGGGGATGCGGTGCCGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACgg                                                                                                                                        <  1:1097958/236‑1 (MQ=255)
                                                                                                          gggggtcgggTGAAGGGTCAGGATAACCGGTGCATCGTCCGTACGTTCCGGGGTGTTTACCCCGGTCGCGGGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAAc                                                                                                                             <  1:951876/231‑1 (MQ=255)
                                                                                                              aTGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCCTCGGCATCTCCCGCCCGCCAAACGGCACCTGATAAACggg                                                                                                                         >  1:899891/1‑239 (MQ=255)
                                                                                                               tGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGCAAGCTGAATG‑CCGCCGGTTCCCCAGCCGGAAGGCAACGGCATCCCCCGCCCGCCCAACGGCCCCTGCTACACgggg                                                                                                                        >  1:1212621/1‑239 (MQ=255)
                                                                                                                 cGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAACAGGCACCGGATAACCGGGGa                                                                                                                       >  1:434082/1‑238 (MQ=255)
                                                                                                                   gTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATTCCGCGCCCCCCAAACGGCACCTGATAACCGGGGATc                                                                                                                     >  1:911697/1‑238 (MQ=255)
                                                                                                                       cgctgttGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCa                                                                                                                 <  1:516529/232‑1 (MQ=255)
                                                                                                                       cgcgTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCa                                                                                                                 <  1:723242/238‑1 (MQ=255)
                                                                                                                         cgTCTGGATACCCGTGGCATCGTCCGTACGTTCCGTGGTGTTTACCCCTGTCGCGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCGGCA‑CGTCGCTTGCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCAcc                                                                                                               <  1:1078187/238‑1 (MQ=255)
                                                                                                                         cgTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCCGCCGTCCGGCCTCGGCATCTCCCCCCCGCCCAACGGCCCCTGGTACCCGGGGTTCGCCAcc                                                                                                               >  1:581450/1‑238 (MQ=255)
                                                                                                                           tCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCCCCGGTTCCCCAGCCGGACGGCATCGGCATTCCCCGCCCCCCCAACGGCACCCGATAAACCGGGGTAGCCACCg                                                                                                              >  1:114380/1‑237 (MQ=255)
                                                                                                                           tCTGGATAACCGGCGCATTCTCCGAACGTTCCGGGGTGTTTACCCCGGTGCCGCGCTTGAAGAAGTTGCGAATCGACACGGCGTCGGGGGTGTCATCCGCG‑CCCTGGGCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATACCGCTGGCGGGTAAGTTG‑CTT‑CCGCACGCTCCACCGCCGCGCGGCATCGGCGTTTCCCGCCCCCCCAACGCCACCCGTAAACCCGGGGGCGccgcacca                                                                                                            >  2:848498/1‑233 (MQ=255)
                                                                                                                                    ccGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCACCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCCCCTGCTAACCGGGGATCGCCACCGCTTTTAAAATg                                                                                                   >  1:812948/1‑239 (MQ=255)
                                                                                                                                         gCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCCCGCCCGCCAAACGGGACCTGATAACCGGGGGTCGCCACCCCCTTTTACATGgccag                                                                                              >  1:1085608/1‑236 (MQ=255)
                                                                                                                                          cattcgccGTACCTTCCGTGGTGTTTCCCCCGGTCCCGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCgcgg                                                                                             <  1:1267019/233‑1 (MQ=255)
                                                                                                                                               tCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCACTTCGCGCCCCCCAAACGGCCCCCGAAAAACGGGGATCGCCACCGCTTTTTAGATGGCGcggcggc                                                                                         >  1:231370/1‑237 (MQ=255)
                                                                                                                                                 cGTACGTTCCGTGGTTTTTGCCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCa                                                                                      <  1:532937/239‑1 (MQ=255)
                                                                                                                                                 cGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCCCCCGATAACCGGGGATCGCCCCCCCTTTTAAGATGGCGCGGCGGATCa                                                                                      >  1:128328/1‑239 (MQ=255)
                                                                                                                                                     cGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAACCTGAATG‑CCGCCGGTTCCCCAGCCGTACGCCACCGGCCTCTCGCGCCCGCCCAACCGCCCCTGACACCCGGGGGACCCCCCCCCTTTTCAAAGGGCGCCGGCGACCATGCg                                                                                  >  2:1037984/1‑239 (MQ=255)
                                                                                                                                                         ccGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGGTCGCCCCCGCTTTTAACATGGCGCGGGGGGTCATGCGTTTg                                                                              >  1:228468/1‑239 (MQ=255)
                                                                                                                                                          cGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCCCCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTT‑CCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCaaa                                                                                                                                           <  1:394581/176‑1 (MQ=255)
                                                                                                                                                          cGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCCACTGTTAACCGGGGGTCGCCACCGCTTTTAAGATGGCGCGGCGGTGCCCGCGTTTgg                                                                             >  1:283059/1‑239 (MQ=255)
                                                                                                                                                          cGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCCCCCGCCAAACGGCACCCGGTAACCGGGGATCGCCACCGCTTTTAAGATGGCGGGGCGGATCATGCGTTggg                                                                             >  1:1294078/1‑239 (MQ=255)
                                                                                                                                                           gtggtgTTTCCCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGGGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGt                                                                            <  1:1100652/239‑1 (MQ=255)
                                                                                                                                                            tggtgTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTAc                                                                                                                                                                     <  1:83229/149‑1 (MQ=255)
                                                                                                                                                              gtttttCCCCCGGGCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCCTCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCt                                                                          <  1:1128223/235‑1 (MQ=255)
                                                                                                                                                               tgtTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCCCCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTg                                                                         <  1:464728/238‑1 (MQ=255)
                                                                                                                                                                 tttACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCCCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGGCTGCtc                                                                      >  1:49021/1‑239 (MQ=255)
                                                                                                                                                                 tttACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGGGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCC‑CGTCCCTTTCGCCAATCACCCTGGC‑GGTAAGCTGAATG‑CCCCCGGTTCCCCAGCCGCACGCCCCCGGCATCTCCCGCCCCCCAAACAGCCCCTGTGACCCGCGGAGCGCCACCCCCTTTGAGGTGCGGTGCCGGGTCATGCGTTggggtggccg                                                                      >  2:827427/1‑232 (MQ=255)
                                                                                                                                                                    aCCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGGTCCCCACCCGTACGGCAACGGCATCCCGCGCCCCCCAAACGGCACCTGCTAACCGGGGATCCCCCCCCCCTTTAAACTGGCCCGGCGGGCCATTCGGTTgggcgtgccgcc                                                                   >  2:763687/1‑229 (MQ=255)
                                                                                                                                                                        cGGTCCCGCGCTTGAAGAAGTTGCGAATCGACCCGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAgg                                                               <  1:539663/239‑1 (MQ=255)
                                                                                                                                                                         ggTCGCGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCCGCCCTACGGCACCGGCCTCTCGCGCCCGCCAAACGGTCCCCGATAACCGGGGGTCGCCACCGCCTTTAAGCTGGGGCCGCGGCCCATACGCTTGGTGTGCtggcggcgga                                                              >  2:983917/1‑230 (MQ=255)
                                                                                                                                                                         ggTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGGACCTGATAACCGGGGATCGCCCCCGCCTTTAAGATGGCGCGGCGGGTCATGCGTTTGGTCTGCTCGTCGAggg                                                              >  1:458181/1‑238 (MQ=255)
                                                                                                                                                                         ggTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGTTAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGt                                                              >  1:924348/1‑239 (MQ=255)
                                                                                                                                                                           tCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCCTGCGTTTGGTCTGCCCGCCGAGGTAg                                                            >  1:899305/1‑239 (MQ=255)
                                                                                                                                                                                cgcTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCCAACGGCACCTGATAACCGGGGCTCGCCACCGCTTTTAAAATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCaaa                                                       >  1:939056/1‑239 (MQ=255)
                                                                                                                                                                                   ttGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACgg                                                                                                                                        <  1:640637/155‑1 (MQ=255)
                                                                                                                                                                                             tGCGAATCGACACGGCGTTGGTGGTGTTATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGCTAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGGTCATGCGTTTGGTCTGCTCGTCGAGGGAGGCAAAGTTGTAGCCGCt                                           >  1:415973/1‑238 (MQ=255)
                                                                                                                                                                                             tGCGAACCCACACGGGGTTGGGGGTGTCATCCGCG‑CCCTGGTCAATCTCCTTCAGCA‑CGTCGCTTTCGCCAAGCCCGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCt                                           <  1:36721/238‑1 (MQ=255)
                                                                                                                                                                                                gAATGGACCCGGCGGTGGTGGTGTCTCCCGCC‑CCCTGGGCAACCCCCTCCAGCC‑GGGCGCCTTCGCCAATCCCGCGGGC‑GGTAAGCGAAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGa                                       <  2:1237394/239‑1 (MQ=255)
                                                                                                                                                                                                      gcatggggTTGGTGGTTTCCACCGCG‑CCCTGGTCAACCCCCTTCCGCA‑CGTCGCTTTCGCCAGTCCCGCTGGC‑GGTAAGCGCAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGcc                                 <  2:1067267/232‑1 (MQ=255)
                                                                                                                                                                                                       cccGGGGTTGGGGGTGTCATCCCGG‑CCCTGGTCAATCCCCTTCAGCC‑CGTCGCTTGCGCCAATCCCGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCa                                <  1:530090/237‑1 (MQ=255)
                                                                                                                                                                                                       cacGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTGCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCa                                <  1:916957/239‑1 (MQ=255)
                                                                                                                                                                                                        ccGGCGTTGGGGGTCGCCTCCGCC‑CCCTGGTACATCCCCTTCAGCC‑CTTCCCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCa                                <  1:1313931/237‑1 (MQ=255)
                                                                                                                                                                                                              ttGGTGGTGTCATCCGCG‑CCCTGGTCAATCCCCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTc                          <  1:405095/238‑1 (MQ=255)
                                                                                                                                                                                                               tggtggTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTg                        <  1:746291/239‑1 (MQ=255)
                                                                                                                                                                                                               tggtggGGTCAGCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCCCGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTg                        <  1:204053/239‑1 (MQ=255)
                                                                                                                                                                                                                      gtCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAACTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCCTTTAAGAGGGCGCGGCGGCTCATGCGTTTGGTCTGCCCCGCGCGGGAAGCAAAGGTGACGCCGCGCAGTGTAGCCCTTGGTCTGCCCgtgt                 >  2:1119697/1‑239 (MQ=255)
                                                                                                                                                                                                                      gtCATCCACG‑CCCTGTTCAACCACCTTCCGCT‑AGGCGCTTTCGCCAATCACGTCGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTg                  <  1:1109991/238‑1 (MQ=255)
                                                                                                                                                                                                                         aTCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTg                                                                         <  1:1227792/180‑1 (MQ=255)
                                                                                                                                                                                                                          cccGCG‑CCCGGGTCTATCACCTTCAGCC‑CGTCCCTTTCGCAACTCCCGCGGGG‑GTTACGCTGAACG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTTATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCa             <  2:415973/238‑1 (MQ=255)
                                                                                                                                                                                                                           tcccccccttGGTCAATCCCCTTCCTCC‑CGTCGCTTTCCCCAATCCCGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTCCGGCATCGGCATCTCTCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCa             <  2:205380/230‑1 (MQ=255)
                                                                                                                                                                                                                           ccccgccccTGGGTAATCCCCTTC‑GCACCGTCGCTGCCCACAATCCCGCTGGG‑GGTAAGGTGAAGT‑CCGCCGGTTCCCCAGGCGTACGGCAACGGCATCTCGCGGCCGCCGAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCa             <  2:1319427/233‑1 (MQ=255)
                                                                                                                                                                                                                           cccccccccTGGACAACACCCTCCAGCC‑CTTCCCTTTCCCCAACCCCGCGGGG‑GGTAACCGGAAGC‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCa             <  2:1122083/233‑1 (MQ=255)
                                                                                                                                                                                                                              cg‑cCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCCGCCGTACGGCCTCGGCATCTCGCGCCCGCCAAACGGCCCCCGATAACCGGGGATCGCCACCCCATTTAAGATGGCGCGGCGGATCATGCGGTTTGTCTGCTCCTCGGGGGAAGCACAGTTGTCGCCGCCCAGCTTTCGCCTGGTTTTGCTCCCCTTGCaacag         >  2:1090418/1‑235 (MQ=255)
                                                                                                                                                                                                                              cg‑cCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGACGGTTCCCCACCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCCCCTGATAACCGGGGATGGGCACCGCTTTCAAGATGGCGCGGCGGATCGCGCGTTTGGACTGCCCGGCGGGGTAGGCAACGTTGTACCACCTCAGACTTGCCACGGTTATCCTCCTGTTggaacag         >  2:569575/1‑233 (MQ=255)
                                                                                                                                                                                                                               g‑cCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCCCGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGt        <  1:1286709/239‑1 (MQ=255)
                                                                                                                                                                                                                                     ggTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGGTTAACCATGGTTTTGCTCCTGTTTCAGACGGTTGAg   >  1:301290/1‑239 (MQ=255)
                                                                                                                                                                                                                                     ggTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAg   <  1:551622/239‑1 (MQ=255)
                                                                                                                                                                                                                                       tCAATCACCTTCAGCA‑CGTCCCTTTGGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGt  <  1:95786/238‑1 (MQ=255)
                                                                                                                                                                                                                                       acAATCCCCTTCCGGC‑CGTGTCTTTCGCCAATCCCCCTGGG‑CGTAACGGTAATG‑CCGCCGGTTCCCCAGCCGTACGGCAACGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGt  <  1:489313/237‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          
GCGCGTGCATGGTGCGGGTTTCCGTTTCGCGCGGCTCGATAAAGCGCAGCGGCTCGGGGATTGGCACCTGGAAGATAATTATCTGATCTTCGGTCAGCGGCGTTTCGGGGATGCGGTGACGCGTCTGGATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCG‑CCCTGGTCAATCACCTTCAGCA‑CGTCGCTTTCGCCAATCACGCTGGC‑GGTAAGCTGAATG‑CCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCGTTTGGTCTGCTCGTCGAGGTAGGCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGT  >  NC_000913/4319168‑4319635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A