breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 37,250 A→C 18.6% V192G (GTG→GGG)  caiC ← putative crotonobetaine/carnitine‑CoA ligase
RA 37,255 A→C 17.5% G190G (GGT→GGG caiC ← putative crotonobetaine/carnitine‑CoA ligase
RA 71,870 A→G 15.5% S174G (AGC→GGC)  yabI → DedA family inner membrane protein
RA 86,765 A→G 10.9% E379G (GAG→GGG)  ilvI → acetolactate synthase 3 large subunit
RA 86,769 T→C 7.3% T380T (ACT→ACC ilvI → acetolactate synthase 3 large subunit
RA 115,063 A→C 8.4% V221G (GTG→GGG)  hofC ← assembly protein in type IV pilin biogenesis, transmembrane protein
RA 154,321 A→C 11.1% V369G (GTC→GGC)  htrE ← putative outer membrane usher protein
RA 218,734 T→G 9.2% T14T (ACA→ACC proS ← prolyl‑tRNA synthetase
RA 252,387 G→A 13.3% K29K (AAG→AAA yafO → mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 319,100 T→A 5.5% Y301F (TAC→TTC)  rclA ← reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
RA 361,668 C→T 16.7% E65K (GAA→AAA)  lacA ← thiogalactoside acetyltransferase
RA 361,728 C→T 14.1% E45K (GAA→AAA)  lacA ← thiogalactoside acetyltransferase
RA 429,830 T→G 18.8% V109G (GTG→GGG)  secF → SecYEG protein translocase auxillary subunit
RA 433,649 T→G 32.7% G65G (GGT→GGG ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
RA 437,444 A→C 18.6% V231G (GTG→GGG)  yajO ← 2‑carboxybenzaldehyde reductase
RA 454,195 T→G 11.7% intergenic (‑28/‑277) yajG ← / → bolA putative lipoprotein/stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC
JC JC 458,790 IS186 (–) +6 bp :: Δ1 bp 80.5% intergenic (+90/‑93) clpX → / → lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
RA 484,581 A→C 9.7% W347G (TGG→GGG)  acrA ← multidrug efflux system
RA 598,428 T→C 19.1% L317P (CTC→CCC)  cusB → copper/silver efflux system, membrane fusion protein
RA 649,330 A→C 11.0% V81G (GTG→GGG)  citF ← citrate lyase, citrate‑ACP transferase (alpha) subunit
RA 665,104 A→C 16.7% S29R (AGT→AGG rlpA ← septal ring protein, suppressor of prc, minor lipoprotein
RA 759,427 A→G 14.8% E241G (GAG→GGG)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
RA 766,738 A→G 28.0% E252G (GAG→GGG)  mngA → fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
RA 825,248 G→C 21.3% G287G (GGC→GGG ybhR ← putative ABC transporter permease
RA 861,108 A→C 16.4% V500G (GTG→GGG)  ybiW ← putative pyruvate formate lyase
RA 871,360 A→G 19.1% S132G (AGT→GGT)  gsiC → glutathione ABC transporter permease
RA 909,181 A→G 13.4% A38A (GCT→GCC ltaE ← L‑allo‑threonine aldolase, PLP‑dependent
RA 956,640 A→C 15.4% intergenic (‑8/‑122) ycaO ← / → ycaP ribosomal protein S12 methylthiotransferase accessory factor/UPF0702 family putative inner membrane protein
RA 1,003,134 T→G 23.0% C82W (TGT→TGG ycbU → putative fimbriae protein
RA 1,058,486 T→C 18.6% S135P (TCG→CCG)  torC → trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
RA 1,123,777 A→C 13.0% V93G (GTC→GGC)  grxB ← glutaredoxin 2 (Grx2)
RA 1,137,894 T→C 18.1% L175P (CTC→CCC)  flgJ → flagellar rod assembly protein and murein hydrolase; flagellum‑specific muramidase
RA 1,196,305 C→T 90.2% R395C (CGT→TGT)  icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,263,180 G→T 18.1% D107E (GAC→GAA lolB ← lipoprotein localization factor
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,357,427 T→C 10.1% E81G (GAG→GGG)  ymjA ← DUF2543 family protein
RA 1,383,718 C→A 22.4% E82* (GAA→TAA)  ycjW ← LacI family putative transcriptional repressor
RA 1,589,069 C→T 19.9% pseudogene (1011/1227 nt) ydeT ← pseudogene; putative outer membrane protein
RA 1,616,835 T→A 16.5% I358K (ATA→AAA)  yneK → uncharacterized protein
RA 1,639,706 A→C 12.1% C118G (TGT→GGT)  rrrQ ← Qin prophage; putative lysozyme
RA 1,912,136 A→C 19.4% G147G (GGT→GGG htpX ← putative endopeptidase
RA 1,915,574 G→A 5.1% intergenic (‑40/+57) proQ ← / ← msrC RNA chaperone, putative ProP translation regulator/free methionine‑(R)‑sulfoxide reductase
RA 1,921,260 T→G 11.9% G346G (GGT→GGG rsmF → 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
RA 1,923,359 A→T 10.5% intergenic (+45/+6) ryeA → / ← yebY novel sRNA, function unknown/DUF2511 family protein
JC JC 1,936,520 IS5 (–) +4 bp 14.0% intergenic (‑206/‑129) zwf ← / → yebK glucose‑6‑phosphate 1‑dehydrogenase/putative DNA‑binding transcriptional regulator
RA 1,976,685 T→C 14.9% E152G (GAG→GGG)  motA ← proton conductor component of flagella motor
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 1,994,236 A→C 11.4% S157R (AGT→AGG uvrC ← excinuclease UvrABC, endonuclease subunit
RA 2,120,560 A→C 11.2% V332G (GTG→GGG)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,145,868 A→G 9.9% E868G (GAG→GGG)  yegE → putative diguanylate cyclase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,219,306 A→C 17.4% V59G (GTG→GGG)  osmF ← putative ABC transporter periplasmic binding protein
RA 2,235,398 T→G 22.6% V45G (GTG→GGG)  preA → dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
RA 2,349,405 T→C 9.2% E23G (GAG→GGG)  inaA ← acid‑inducible Kdo/WaaP family putative kinase
RA 2,459,225 T→C 21.9% G415G (GGA→GGG fadI ← beta‑ketoacyl‑CoA thiolase, anaerobic, subunit
RA 2,504,892 A→C 17.1% V219G (GTG→GGG)  ypdE ← aminopeptidase
RA 2,513,129 A→T 9.2% I30F (ATC→TTC)  nupC → nucleoside (except guanosine) transporter
RA 2,541,270 T→C 15.8% E137G (GAG→GGG)  cysW ← sulfate/thiosulfate ABC transporter permease
RA 2,623,867 T→G 11.8% V275G (GTG→GGG)  ppk → polyphosphate kinase, component of RNA degradosome
RA 2,704,841 T→G 12.3% T157P (ACT→CCT)  lepB ← leader peptidase (signal peptidase I)
RA 2,739,684 T→C 13.0% E129G (GAG→GGG)  tyrA ← fused chorismate mutase T/prephenate dehydrogenase
RA 2,777,459 A→C 14.5% T3P (ACT→CCT)  ypjF → CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system
RA 2,872,180 A→C 13.1% V104G (GTG→GGG)  ispD ← 4‑diphosphocytidyl‑2C‑methyl‑D‑erythritol synthase
RA 2,877,027 T→G 15.1% G149G (GGT→GGG iap → aminopeptidase in alkaline phosphatase isozyme conversion
RA 2,913,659 T→C 13.6% intergenic (‑8/+40) relA ← / ← rlmD (p)ppGpp synthetase I/GTP pyrophosphokinase/23S rRNA m(5)U1939 methyltransferase, SAM‑dependent
RA 2,944,711 A→G 10.7% E57G (GAG→GGG)  csdE → CsdA‑binding activator; Fe‑S protein
RA 2,952,350 C→G 12.9% A38P (GCG→CCG)  recD ← exonuclease V (RecBCD complex), alpha chain
RA 2,967,845 A→C 19.2% V197G (GTG→GGG)  ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
RA 3,051,839 A→C 12.8% V160G (GTG→GGG)  ubiI ← 2‑octaprenylphenol hydroxylase, FAD‑dependent
RA 3,062,718 A→C 13.6% T623T (ACA→ACC scpA → methylmalonyl‑CoA mutase
RA 3,141,137 G→C 15.6% G50G (GGC→GGG hybD ← maturation protease for hydrogenase 2
RA 3,206,452 C→A 17.3% intergenic (‑196/‑11) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,206,471 C→G 17.3% S3R (AGC→AGG ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,483 G→A 16.8% K7K (AAG→AAA ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,506 C→A 17.1% T15K (ACA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,209,180 T→G 14.0% V386G (GTG→GGG)  ttdT → L‑tartrate/succinate antiporte
RA 3,265,360 T→C 18.2% G223G (GGA→GGG tdcB ← L‑threonine dehydratase, catabolic
RA 3,289,078 C→A 13.8% Q89K (CAG→AAG)  yraJ → putative outer membrane protein
RA 3,317,603 A→C 13.0% V135G (GTG→GGG)  rimP ← ribosome maturation factor for 30S subunits
RA 3,319,873 A→C 9.9% T413P (ACT→CCT)  argG → argininosuccinate synthetase
RA 3,332,561 T→C 16.6% R59G (AGG→GGG)  yhbE ← EamA family inner membrane putative transporter
RA 3,338,869 T→C 12.3% E127G (GAG→GGG)  mlaE ← ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
RA 3,344,984 A→C 15.9% T90P (ACC→CCC)  rpoN → RNA polymerase, sigma 54 (sigma N) factor
RA 3,395,441 T→C 11.8% E273G (GAG→GGG)  yhdP ← DUF3971‑AsmA2 domains protein
RA 3,405,781 T→G 13.1% C116G (TGC→GGC)  accB → acetyl CoA carboxylase, BCCP subunit
RA 3,416,434 C→T 17.7% R468C (CGC→TGC)  acrF → multidrug efflux system protein
RA 3,438,597 G→T 6.6% A18E (GCA→GAA)  arfA ← alternate ribosome‑rescue factor A
RA 3,439,039 A→C 11.2% V31G (GTG→GGG)  zntR ← zntA gene transcriptional activator
RA 3,460,787 T→C 15.5% F324S (TTC→TCC)  gspF → general secretory pathway component, cryptic
RA 3,480,296 T→C 21.1% G104G (GGA→GGG kefB ← potassium:proton antiporter
RA 3,499,651 G→T 22.9% intergenic (+36/‑259) yhfL → / → frlA small lipoprotein/putative fructoselysine transporter
RA 3,499,694 G→T 11.7% intergenic (+79/‑216) yhfL → / → frlA small lipoprotein/putative fructoselysine transporter
RA 3,520,597 A→C 9.0% V36G (GTG→GGG)  hofQ ← DNA catabolic putative fimbrial transporter
RA 3,536,135 G→C 22.2% R150R (CGC→CGG ompR ← response regulator in two‑component regulatory system with EnvZ
RA 3,553,285 T→G 16.0% G67G (GGT→GGG malT → mal regulon transcriptional activator
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,638,228 A→G 17.5% E196G (GAG→GGG)  pitA → phosphate transporter, low‑affinity; tellurite importer
RA 3,672,789 A→G 6.8% S126G (AGT→GGT)  yhjC → LysR family putative transcriptional regulator
RA 3,686,539 T→C 10.9% S879G (AGC→GGC)  bcsC ← cellulose synthase subunit
RA 3,699,212 G→T 13.4% L333F (TTG→TTT bcsG → DUF3260 family cellulose production inner membrane protein
RA 3,749,429 T→G 18.8% G66G (GGT→GGG sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase
RA 3,765,494 T→G 25.8% S1104R (AGT→AGG rhsA → Rhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
RA 3,784,900 A→C 12.9% V39G (GTG→GGG)  yibN ← putative rhodanese‑related sulfurtransferase
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,854,898 A→G 19.4% E326G (GAG→GGG)  emrD → multidrug efflux system protein
RA 3,862,674 C→A 35.7% pseudogene (930/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
RA 3,862,677 G→C 26.2% pseudogene (927/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
RA 3,880,848 A→G 9.8% L125P (CTC→CCC)  recF ← gap repair protein
RA 3,975,377 A→G 22.9% S78G (AGC→GGC)  wecE → TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
RA 3,975,383 A→C 19.4% T80P (ACC→CCC)  wecE → TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
RA 3,981,335 A→G 14.6% E150G (GAG→GGG)  yifK → putative APC family amino acid transporter
JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,013,577 T→G 12.1% G175G (GGT→GGG metE → 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
RA 4,046,374 G→A 8.9% L77L (CTG→TTG)  yihG ← inner membrane protein, inner membrane acyltransferase
RA 4,065,571 T→A 17.1% T66S (ACC→TCC)  yihO ← putative sulphoquinovose importer
RA 4,072,425 T→C 8.8% E76G (GAG→GGG)  yihT ← 6‑deoxy‑6‑sulphofructose‑1‑phosphate aldolase
RA 4,086,203 A→G 11.4% E63G (GAG→GGG)  fdhD → formate dehydrogenase formation protein
RA 4,101,255 C→T 9.6% S149F (TCT→TTT)  sodA → superoxide dismutase, Mn
RA 4,103,633 A→C 15.1% V448G (GTG→GGG)  cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR
RA 4,135,882 T→G 9.3% V683G (GTG→GGG)  katG → catalase‑peroxidase HPI, heme b‑containing
RA 4,140,198 T→G 15.1% H675P (CAC→CCC)  frwA ← putative PTS enzyme: Hpr, enzyme I and II components
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,192,034 A→C 17.6% V201G (GTG→GGG)  thiG ← thiamine biosynthesis ThiGH complex subunit
RA 4,201,278 A→C 20.6% G137G (GGT→GGG zraP ← Zn‑dependent periplasmic chaperone
RA 4,219,539 C→T 18.4% A315V (GCG→GTG)  aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,241,927 T→A 10.0% T110S (ACC→TCC)  xylE ← D‑xylose transporter
RA 4,241,998 T→C 12.9% D86G (GAT→GGT)  xylE ← D‑xylose transporter
RA 4,287,460 C→A 6.4% intergenic (‑89/‑304) acs ← / → nrfA acetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome
RA 4,295,700 T→G 16.3% V407G (GTG→GGG)  gltP → glutamate/aspartate:proton symporter
RA 4,296,060 C→T 20.0% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,317,498 A→G 14.9% L133P (CTC→CCC)  phnL ← ribophosphonate triphosphate synthase subunit; putative ABC transporter‑related ATPase
RA 4,319,404 A→C 11.7% V68G (GTG→GGG)  phnJ ← carbon‑phosphorus lyase, SAM‑dependent
RA 4,331,615 A→T 13.3% M372L (ATG→TTG)  proP → proline/glycine betaine transporter
RA 4,347,142 A→G 12.4% L62P (CTC→CCC)  fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,401,809 A→G 9.2% R380G (AGA→GGA)  hflX → GTPase, stimulated by 50S subunit binding; Mn(2+) homeostasis regulator
RA 4,424,136 T→G 12.5% V146G (GTG→GGG)  ulaF → L‑ribulose 5‑phosphate 4‑epimerase
RA 4,429,997 C→T 15.8% S45F (TCT→TTT)  cycA → D‑alanine/D‑serine/glycine transporter
RA 4,480,111 C→T 9.8% L128F (CTC→TTC)  yjgN → DUF898 family inner membrane protein
RA 4,487,250 C→T 9.9% L345F (CTC→TTC)  lptF → lipopolysaccharide export ABC permease
RA 4,489,888 A→C 11.8% V59G (GTG→GGG)  yjgR ← DUF853 family protein with NTPase fold
RA 4,502,617 A→C 12.2% V272G (GTG→GGG)  insG ← IS4 transposase
RA 4,542,223 C→T 12.2% R63C (CGC→TGC)  fimE → tyrosine recombinase/inversion of on/off regulator of fimA
RA 4,587,542 A→G 14.3% G198G (GGA→GGG mrr → methylated adenine and cytosine restriction protein
RA 4,588,079 A→C 14.2% W263G (TGG→GGG)  yjiA ← metal‑binding GTPase
RA 4,596,352 T→G 18.2% S121R (AGT→AGG lgoD → L‑galactonate oxidoreductase; L‑gulonate oxidoreductase
RA 4,625,085 T→G 13.6% V64G (GTG→GGG)  serB → 3‑phosphoserine phosphatase
RA 4,631,505 A→G 11.4% E258G (GAG→GGG)  slt → lytic murein transglycosylase, soluble

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 299950 300198 249 25 [22] [22] 25 paoC PaoABC aldehyde oxidoreductase, Moco‑containing subunit
* * ÷ NC_000913 583142 583744 603 25 [21] [23] 24 [appY] [appY]
* * ÷ NC_000913 1212034 1212545 512 26 [22] [22] 24 iraM/ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
* * ÷ NC_000913 1812686 1812936 251 26 [22] [23] 24 ydjO uncharacterized protein
* * ÷ NC_000913 1978500 1979278 779 26 [23] [5] 28 insB1–insA insB1,insA
* * ÷ NC_000913 2455625 2456122 498 24 [22] [18] 26 [yfcV] [yfcV]
* * ÷ NC_000913 2469139 2469785 647 34 [20] [21] 25 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ NC_000913 3653663 3653842 180 24 [23] [22] 24 [yhiS] [yhiS]
* * ÷ NC_000913 3798982 3799507 526 24 [20] [12] 25 [waaU]–[waaZ] [waaU],[waaZ]
* * ÷ NC_000913 4233747 4235542 1796 25 [23] [23] 25 pgi pgi

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 279163 =NA (NA)64 (0.670) 3/432 NT 48.5% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 2760086 68 (0.710)coding (1102/1410 nt) yfjI CP4‑57 prophage; uncharacterized protein
* ? NC_000913 1207790 =29 (0.300)71 (0.800) 53/400 NT 77.7% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 14 (0.160)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780529 (0.300)58 (0.650) 41/400 NT 74.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 14 (0.160)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)25 (0.270) 21/424 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19785020 (0.000)23 (0.250) 19/416 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein