Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	502	34.8	1912488	98.2%	1878063	80.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,882,617	T→C	A7A (GCA→GCG)	casB ←	type I‑E CRISPR system Cascade subunit CasB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,882,617	0	T	C	95.7%	75.3 / NA	23	A7A (GCA→GCG)	casB	type I‑E CRISPR system Cascade subunit CasB
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (10/12); total (11/12)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATTGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGGCGCG  >  NC_000913/2882540‑2882692

tcatcaGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCa                                                                          >  2:520348/1‑81 (MQ=255)

  atcaGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCAt                                                                         >  1:520353/1‑80 (MQ=255)

  atcaGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCATc                                                                        <  1:520323/81‑1 (MQ=255)

                       aaTTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATg                                                   >  2:520350/1‑81 (MQ=255)

                        aTTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATgg                                                  <  1:520324/81‑1 (MQ=255)

                          ttGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGcc                                                >  2:520354/1‑81 (MQ=255)

                             cgcACATGATCCATTATCCTGTTGTTGCCAGGCTCGATATAAAGCCATTGCATCAATTTCATCAGCCATTTGATGGCcctc                                             >  2:520359/1‑81 (MQ=255)

                                acaTGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCtt                                          <  2:520325/81‑1 (MQ=255)

                                                     ttgCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTaaa                      <  2:520327/80‑1 (MQ=255)

                                                     ttgCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAAt                     <  1:520328/81‑1 (MQ=255)

                                                     ttgCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAAt                     <  1:520326/81‑1 (MQ=255)

                                                          aGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTg                >  1:520362/1‑81 (MQ=255)

                                                             cTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGtat             <  2:520329/81‑1 (MQ=255)

                                                                  tataAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGt        <  2:520330/81‑1 (MQ=255)

                                                                    taAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTgg      <  1:520332/81‑1 (MQ=255)

                                                                    taAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTgg      >  2:520372/1‑81 (MQ=255)

                                                                    taAAGCCATCGCATCAATTTCATCAGCCATATGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTg       <  2:520331/80‑1 (MQ=255)

                                                                      aaGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGgcg    <  2:520333/81‑1 (MQ=255)

                                                                      aaGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGgc     <  1:520334/80‑1 (MQ=255)

                                                                       aGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGgcgc   >  1:520368/1‑81 (MQ=255)

                                                                        gCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGGcgcg  >  1:520358/1‑81 (MQ=255)

                                                                        gCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGGcgcg  >  1:520357/1‑81 (MQ=255)

                                                                        gCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGGcgcg  >  2:520361/1‑81 (MQ=255)

TCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATTGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGGCGCG  >  NC_000913/2882540‑2882692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A