Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,080,994 |
G→A |
R214R (CGC→CGT) |
tktA ← |
transketolase 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,080,994 | 0 | G | A | 100.0%
| 178.5
/ NA
| 49 | R214R (CGC→CGT) | tktA | transketolase 1 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/24); total (25/24) |
GGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCG > NC_000913/3080919‑3081069
|
ggAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAAt < 2:563247/81‑1 (MQ=255)
aaGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATaa > 2:563323/1‑81 (MQ=255)
aGGTTTGTCAGTCACTGCGCGCGCCTCCTCTACTGCGCGTCTGATAGATGCCGCGTCATGACCGTCGATCTCACGAATAAc > 2:563309/1‑81 (MQ=255)
tGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGc > 1:563300/1‑80 (MQ=255)
gtcagtcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGcc < 2:563248/80‑1 (MQ=255)
gtcagtcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGc > 2:563332/1‑79 (MQ=255)
tcagtcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCa < 1:563249/80‑1 (MQ=255)
gtcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCg > 1:563292/1‑81 (MQ=255)
tcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCg > 2:563314/1‑80 (MQ=255)
caCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGt > 2:563326/1‑80 (MQ=255)
aCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTa < 1:563250/80‑1 (MQ=255)
gcgcgcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCt > 1:563313/1‑81 (MQ=255)
cgcgcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCtt < 2:563252/81‑1 (MQ=255)
cgcgcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCtt < 2:563251/81‑1 (MQ=255)
gcgcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTc > 1:563308/1‑81 (MQ=255)
tcttctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACg > 2:563334/1‑80 (MQ=255)
tcttctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACg > 1:563298/1‑80 (MQ=255)
cttctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACg < 1:563253/79‑1 (MQ=255)
ttctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCAt > 2:563329/1‑81 (MQ=255)
ctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCAt < 1:563254/79‑1 (MQ=255)
aCTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCg > 2:563302/1‑81 (MQ=255)
cTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGc < 1:563255/79‑1 (MQ=255)
cTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCg < 1:563256/80‑1 (MQ=255)
tGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgt > 1:563304/1‑81 (MQ=255)
gcgcGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgtg > 1:563343/1‑81 (MQ=255)
gcgcGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgtg > 1:563312/1‑81 (MQ=255)
gcgTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtc < 1:563258/81‑1 (MQ=255)
gcgTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtc < 1:563257/81‑1 (MQ=255)
gTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtcgt > 2:563327/1‑81 (MQ=255)
ttGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGtcgtcg > 2:563311/1‑81 (MQ=255)
tGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGtcgtcg < 2:563260/80‑1 (MQ=255)
gATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAAc < 1:563261/81‑1 (MQ=255)
aTGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAAcc < 2:563262/81‑1 (MQ=255)
tGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAAc > 1:563320/1‑79 (MQ=255)
tGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCa < 2:563264/81‑1 (MQ=255)
tGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCa < 1:563263/81‑1 (MQ=255)
gCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAATCAg > 1:563331/1‑81 (MQ=255)
gcgTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCt > 2:563324/1‑81 (MQ=255)
cgTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCtt < 2:563265/81‑1 (MQ=255)
gTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCtt > 2:563316/1‑80 (MQ=255)
gTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCtt > 2:563328/1‑80 (MQ=255)
tCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCa < 2:563266/81‑1 (MQ=255)
gTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGAc > 1:563305/1‑80 (MQ=255)
tCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCa < 2:563267/81‑1 (MQ=255)
cGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCAt > 2:563330/1‑81 (MQ=255)
gATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCAt < 1:563268/80‑1 (MQ=255)
gATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATc < 2:563269/81‑1 (MQ=255)
aTGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCg < 2:563270/81‑1 (MQ=255)
aTGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCg < 1:563271/81‑1 (MQ=255)
|
GGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCG > NC_000913/3080919‑3081069
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
