Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,168,013 |
(T)6→5 |
coding (234/396 nt) |
mqsA ← |
antitoxin of the MqsRA toxin‑antitoxin system/DNA‑binding transcriptional repressor MqsA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,168,008 | 0 | T | . | 100.0%
| 60.7
/ NA
| 15 | coding (239/396 nt) | mqsA | antitoxin of the MqsRA toxin‑antitoxin system/DNA‑binding transcriptional repressor MqsA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (9/6); total (9/6) |
ATTGCCTTTTTCGTAACGCGAAAACGCATTTACACCTCCCCCAAAAATTTCGCTTGCCTCTTTTTGGGTAAGAGAGAGCTTTTTTCGAACCTTCACTATAAATTCAGGTGCCACTGTTTCGGCATTCACCGAAGCCCGAAATGCCTTTACTTGCG > NC_000913/3167929‑3168083
|
aTTGCCTTTTTCGTAACGCGAAAACGCATTTACACCTCCCCCAAAAATTTCGCTTGCCTCTTTTTGGGTAAGAGAGAGCtt < 2:583947/81‑1 (MQ=255)
ttCGTAACGCGAAAACGCATTTACACCTCCCCCAAAAATTTCGCTTGCCTCTTTTTGGGTAAGAGAGAGC‑TTTTTCGAAcc > 2:583973/1‑81 (MQ=255)
gAAAACGCATTTACACCTCCCCCAAAAATTTCGCTTGCCTCTTTTTGGGTAAGAGAGAGC‑TTTTTCGAACCTTCACTATaa < 1:583948/81‑1 (MQ=255)
aaCGCATTTACACCTCCCCCAAAAATTTCGCTTGCCTCTTTTTGGGTAAGAGAGAGC‑TTTTTCGAACCTTCACTATAAAtt > 2:583974/1‑81 (MQ=255)
cGCATTTACACCTCCCCCAAAAATTTCGCTTGCCTCTTTTTGGGTAAGAGAGAGC‑TTTTTCGAACCTTCACTATAAATTCa < 1:583949/81‑1 (MQ=255)
tttACACCTCCCCCAAAAATTTCGCTTGCCTCTTTTTGGGTAAGAGAGAGC‑TTTTTCGAACCTTCACTATAAATTCAGGTg < 1:583950/81‑1 (MQ=255)
cccccAAAAATTTCGCTTGCCTCTTTTTGGGTAAGAGAGAGC‑TTTTTCGAACCTTCACTATAAATTCAGGTGCCACTGttt > 1:583975/1‑81 (MQ=255)
aaaaTTTCGCTTGCCTCTTTTTGGGTAAGAGAGAGC‑TTTTTCGAACCTTCACTATAAATTCAGGTGCCACTGTTTCGGCAt < 1:583951/81‑1 (MQ=255)
aaaTTTCGCTTGCCTCTTTTTGGGTAAGAGAGAGC‑TTTTTCGAACCTTCACTATAAATTCAGGTGCCACTGTTTCGGCAtt > 1:583982/1‑81 (MQ=255)
aTTTCGCTTGCCTCTTTTTGGGTAAGAGAGAGC‑TTTTTCGAACCTTCACTATAAATTCAGGTGCCACTGTTTCGGCATTCa > 1:583979/1‑81 (MQ=255)
ttCGCTTGCCTCTTTTTGGGTAAGAGAGAGC‑TTTTTCGAACCTTCACTATAAATTCAGGTGCCACTGTTTCGGCATTCAcc > 1:583972/1‑81 (MQ=255)
gCCTCTTTTTGGGTAAGAGAGAGC‑TTTTTCGAACCTTCACTATAAATTCAGTTGCCACTGTTTCGGCATTCACCGAAGc < 2:583952/79‑1 (MQ=255)
ccTCTTTTTGGGTAAGAGAGAGC‑TTTTTCGAACCTTCACTATAAATTCAGGTGCCACTGTTTCGGCATTCACCGAAGccc > 1:583977/1‑80 (MQ=255)
tttGGGTAAGAGAGAGC‑TTTTTCGAACCTTCACTATAAATTCAGGTGCCACTGTTTCGGCATTCACCGAAGCCCGAAAt < 1:583953/79‑1 (MQ=255)
aaGAGAGAGC‑TTTTTCGAACCTTCACTATAAATTCAGGTGCCACTGTTTCGGCATTCACCGAAGCCCGAAATGCCTTTACt > 1:583980/1‑81 (MQ=255)
gagagC‑TTTTTCGAACCTTCACTATAAATTCAGGTGCCACTGTTTCGGCATTCACCGAAGCCCGAAATGCCTTTACTTgcg > 1:583984/1‑81 (MQ=255)
|
ATTGCCTTTTTCGTAACGCGAAAACGCATTTACACCTCCCCCAAAAATTTCGCTTGCCTCTTTTTGGGTAAGAGAGAGCTTTTTTCGAACCTTCACTATAAATTCAGGTGCCACTGTTTCGGCATTCACCGAAGCCCGAAATGCCTTTACTTGCG > NC_000913/3167929‑3168083
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
