Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,178,531 |
G→A |
L139L (TTG→TTA) |
tolC → |
outer membrane channel TolC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,178,531 | 0 | G | A | 100.0%
| 150.8
/ NA
| 41 | L139L (TTG→TTA) | tolC | outer membrane channel TolC |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/22); total (19/22) |
CAGGACGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTGAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGATCAAACCACCCAAC > NC_000913/3178460‑3178607
|
cAGGACGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTAt < 2:586370/80‑1 (MQ=255)
aGGACGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTAtt > 2:586422/1‑80 (MQ=255)
aGGACGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTAtt > 2:586434/1‑80 (MQ=255)
aGGACGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTg < 2:586371/81‑1 (MQ=255)
ggACGTCACGTATCAGACCGATCAGCAAACATTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGa < 1:586372/81‑1 (MQ=255)
cGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGt < 1:586373/81‑1 (MQ=255)
cGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGt < 1:586374/81‑1 (MQ=255)
gTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGtt < 1:586375/81‑1 (MQ=255)
tCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTc < 2:586376/81‑1 (MQ=255)
aCGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCtt < 1:586377/81‑1 (MQ=255)
aCGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCtt > 2:586420/1‑81 (MQ=255)
gTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTc > 1:586424/1‑81 (MQ=255)
tATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTcc < 1:586378/81‑1 (MQ=255)
tATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTcc < 2:586379/81‑1 (MQ=255)
aGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCtata > 1:586423/1‑81 (MQ=255)
aGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCtat < 2:586382/80‑1 (MQ=255)
aGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCtat < 2:586381/80‑1 (MQ=255)
aGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCtat < 1:586380/80‑1 (MQ=255)
aCCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATaca > 1:586429/1‑81 (MQ=255)
tCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAgg > 2:586448/1‑80 (MQ=255)
aaaCCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACaa > 2:586451/1‑80 (MQ=255)
aaaCCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACaa > 2:586442/1‑80 (MQ=255)
aaCCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACaaa > 2:586428/1‑80 (MQ=255)
ttGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAaag > 2:586427/1‑79 (MQ=255)
gATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGc > 1:586437/1‑81 (MQ=255)
cTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCGTATACACAGGCACAAAAAGAAGCGATc > 2:586449/1‑81 (MQ=255)
cTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATc < 2:586383/81‑1 (MQ=255)
cTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATc < 2:586384/81‑1 (MQ=255)
cAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTa > 1:586447/1‑81 (MQ=255)
aaCACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCt < 1:586385/79‑1 (MQ=255)
aaCACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTAc > 2:586433/1‑81 (MQ=255)
cgcgACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCa < 2:586386/81‑1 (MQ=255)
gACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCATCAAt > 2:586436/1‑80 (MQ=255)
aCCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTa < 1:586387/81‑1 (MQ=255)
gCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGAt < 1:586388/81‑1 (MQ=255)
cTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGAt > 1:586443/1‑80 (MQ=255)
aTTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGATCaaa > 2:586445/1‑81 (MQ=255)
tttCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGATCAAac > 2:586446/1‑81 (MQ=255)
cAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGATCAAacca < 2:586389/80‑1 (MQ=255)
tgtTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGTTCTACCGTCAATTAGATCAAACCACCCAAc < 2:586391/81‑1 (MQ=255)
tgtTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGATCAAACCACCCAAc < 2:586390/81‑1 (MQ=255)
|
CAGGACGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTGAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGATCAAACCACCCAAC > NC_000913/3178460‑3178607
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
