Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,268,686 |
(T)6→7 |
coding (272/1188 nt) |
yhaC → |
uncharacterized protein YhaC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,268,680 | 1 | . | T | 100.0%
| 49.8
/ NA
| 15 | D89V (GAT→GTT) | yhaC | uncharacterized protein YhaC |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (10/5); total (10/5) |
ATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGA‑TTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAA > NC_000913/3268601‑3268754
|
aTTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGA‑t < 1:606889/81‑1 (MQ=255)
ttATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGA‑tt > 2:606910/1‑81 (MQ=255)
tttCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTTAGGTTATaa > 1:606916/1‑81 (MQ=255)
aGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTTAGGTTATAACGCCCAAattatt < 2:606890/80‑1 (MQ=255)
gTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTTAGGTTATAACGCCCAAATTATTCAAc > 1:606913/1‑81 (MQ=255)
aCAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTTAGGTTATAACGCCCAAATTATTCAACGATc < 2:606891/79‑1 (MQ=255)
aTGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACaa > 1:606918/1‑80 (MQ=255)
ttCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTtgt < 1:606892/81‑1 (MQ=255)
tataATGTCTCGTCTCTTCGTCTGGATTTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACt > 2:606911/1‑81 (MQ=255)
ataATGTCTCGTCTCTTCGTCTGGATTTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACt > 1:606914/1‑80 (MQ=255)
ataATGTCTCGTCTCTTCGTCTGGATTTTTTTAGGATATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACt < 2:606893/80‑1 (MQ=255)
taATGTCTCGTCTCTTCGTCTGGATTTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACtt < 2:606894/80‑1 (MQ=255)
taATGTCTCGTCTCTTCGTCTGGATTTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTa > 2:606921/1‑81 (MQ=255)
aaTGTCTCGTCTCTTCGTCTGGATTTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTAc > 2:606912/1‑81 (MQ=255)
cgtctcTTCGTCTGGATTTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAAt > 2:606917/1‑81 (MQ=255)
cgtctcTTCGTCTGGATTTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAAt > 2:606919/1‑81 (MQ=255)
cTGGATTTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaa > 2:606920/1‑80 (MQ=255)
|
ATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGA‑TTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAA > NC_000913/3268601‑3268754
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
