Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,269,345 |
A→C |
I311L (ATC→CTC) |
yhaC → |
uncharacterized protein YhaC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,269,345 | 0 | A | C | 84.2%
| 52.4
/ 3.0
| 19 | I311L (ATC→CTC) | yhaC | uncharacterized protein YhaC |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base C (9/7); total (11/8) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATA > NC_000913/3269271‑3269425
|
aTTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATACTCAtt > 2:549569/1‑80 (MQ=255)
agaTGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATg < 2:549539/81‑1 (MQ=255)
aTGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCt < 1:549541/81‑1 (MQ=255)
aaGAATTCTCATCAGAAAAAATATATGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATc > 1:549560/1‑81 (MQ=255)
aaTTCTCATCAGAAAAAATATATGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTgg > 1:549569/1‑81 (MQ=255)
catcaGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTcc > 2:549552/1‑81 (MQ=255)
atcaGAAAAAATATATGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGgtcgtc < 2:549541/79‑1 (MQ=255)
aaaaaaTATATGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCaaa < 1:549543/81‑1 (MQ=255)
atGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATtata > 1:549574/1‑81 (MQ=255)
atGATTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATtata > 2:549571/1‑81 (MQ=255)
aTTACATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTa > 2:549564/1‑81 (MQ=255)
aCATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATg > 1:549568/1‑80 (MQ=255)
aCATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATg < 1:549544/80‑1 (MQ=255)
cATCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATgg < 1:549545/80‑1 (MQ=255)
aTCGATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGAt > 2:549565/1‑81 (MQ=255)
gATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGAttt < 2:549545/80‑1 (MQ=255)
gATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGAttt > 2:549570/1‑80 (MQ=255)
gATATACTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTa < 2:549546/81‑1 (MQ=255)
tataATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTaaa > 2:549572/1‑81 (MQ=255)
aTCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATa < 1:549548/81‑1 (MQ=255)
|
ATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATA > NC_000913/3269271‑3269425
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
