Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,356,205 |
T→A |
V494E (GTG→GAG) |
gltB → |
glutamate synthase subunit GltB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,356,205 | 0 | T | A | 100.0%
| 111.1
/ NA
| 31 | V494E (GTG→GAG) | gltB | glutamate synthase subunit GltB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (12/19); total (12/19) |
GGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGA > NC_000913/3356128‑3356280
|
ggACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGTGATGATACCCCATTCGCCGAGct > 2:627658/1‑81 (MQ=255)
gACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGc < 2:627616/79‑1 (MQ=255)
cGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTcc < 1:627617/79‑1 (MQ=255)
cGTAATTCGAGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAg > 2:627650/1‑81 (MQ=255)
gTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGt < 2:627618/81‑1 (MQ=255)
tCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGcc > 2:627657/1‑81 (MQ=255)
cgcgTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGcc < 1:627619/80‑1 (MQ=255)
gcgTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCcgc < 2:627620/81‑1 (MQ=255)
gTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCgcgc < 1:627621/81‑1 (MQ=255)
tACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCa > 1:627653/1‑81 (MQ=255)
cTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCat > 1:627661/1‑80 (MQ=255)
cTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCat < 2:627622/80‑1 (MQ=255)
aaCGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGac > 2:627656/1‑81 (MQ=255)
aCGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGact < 1:627623/81‑1 (MQ=255)
aCGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGact < 1:627625/81‑1 (MQ=255)
aCGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGact < 1:627624/81‑1 (MQ=255)
tCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACtt < 2:627626/81‑1 (MQ=255)
aGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGc > 2:627660/1‑79 (MQ=255)
aGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCca < 2:627627/81‑1 (MQ=255)
ggtcggtTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCcag > 2:627663/1‑79 (MQ=255)
gtTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTg < 1:627628/81‑1 (MQ=255)
tGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAgg < 1:627629/81‑1 (MQ=255)
ggCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTg < 1:627630/81‑1 (MQ=255)
atgatACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGATTACTTCCGCCAGCAGTTTGCCCAGGTGACt > 2:627665/1‑80 (MQ=255)
atACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAAc < 1:627631/80‑1 (MQ=255)
cccATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGcc < 1:627632/81‑1 (MQ=255)
aTTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAAt > 1:627681/1‑81 (MQ=255)
aTTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTCCCAAGGTGACTAACCCGCCAAt < 2:627633/81‑1 (MQ=255)
tCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATc > 2:627676/1‑80 (MQ=255)
cGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGa > 1:627673/1‑81 (MQ=255)
cGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTACCCAGGTGACTAACCCGCCAATCg < 2:627634/80‑1 (MQ=255)
|
GGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGA > NC_000913/3356128‑3356280
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
