Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,567,517 |
G→A |
H173Y (CAC→TAC) |
glgA ← |
glycogen synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,567,517 | 0 | G | A | 100.0%
| 138.1
/ NA
| 38 | H173Y (CAC→TAC) | glgA | glycogen synthase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/21); total (17/21) |
GGAAAGAGATTTGTCCGTTGAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGCCGC > NC_000913/3567442‑3567592
|
ggAAAGAGATTTGTCCGTTGAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCatgtaatgtg > 1:678550/1‑81 (MQ=255)
agagATTTGTCCGTTGAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCAt > 1:678564/1‑80 (MQ=255)
gagaTTTGTCCGTTGAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATa > 1:678559/1‑80 (MQ=255)
tttGTCCGTTGAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACa < 1:678516/81‑1 (MQ=255)
ccGTTGAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCt > 1:678575/1‑81 (MQ=255)
cGTTGAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCt < 1:678517/80‑1 (MQ=255)
gTTGAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTg < 2:678518/81‑1 (MQ=255)
ttGAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGa < 2:678520/81‑1 (MQ=255)
ttGAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGa < 1:678521/81‑1 (MQ=255)
ttGAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGa < 1:678519/81‑1 (MQ=255)
cAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCa < 1:678522/80‑1 (MQ=255)
cAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAg < 1:678523/81‑1 (MQ=255)
aGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAgg > 2:678573/1‑81 (MQ=255)
gCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGt < 2:678524/81‑1 (MQ=255)
cATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTtgt < 1:678525/81‑1 (MQ=255)
gAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGca > 2:678567/1‑81 (MQ=255)
aaTATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGca < 2:678526/80‑1 (MQ=255)
aaTATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGca > 1:678571/1‑80 (MQ=255)
aaTATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGca < 1:678527/80‑1 (MQ=255)
atTAAAGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAg > 1:678566/1‑80 (MQ=255)
aaaGAATGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTaaa > 2:678565/1‑81 (MQ=255)
aaTGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACAcc > 1:678558/1‑81 (MQ=255)
aaTGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACAcc < 1:678528/81‑1 (MQ=255)
tGACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGa < 1:678529/81‑1 (MQ=255)
gACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGAc > 2:678562/1‑81 (MQ=255)
gACCATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGAc > 1:678563/1‑81 (MQ=255)
ccATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACtt > 1:678561/1‑81 (MQ=255)
cATGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTc < 2:678530/81‑1 (MQ=255)
tGGCAATTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACTGACTTCGc < 1:678531/81‑1 (MQ=255)
aTTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCATAGTAAACACCGATTTCGCCGTAc > 1:678580/1‑81 (MQ=255)
aTTGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGAc > 1:678579/1‑81 (MQ=255)
ttGGATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACg < 1:678532/81‑1 (MQ=255)
gATGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGccc < 1:678533/81‑1 (MQ=255)
aTGTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCcg < 2:678534/81‑1 (MQ=255)
gTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCcgcg > 1:678586/1‑81 (MQ=255)
gTCATTCATGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCcgcg > 2:678577/1‑81 (MQ=255)
tcattcatGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCcgcgc < 2:678535/81‑1 (MQ=255)
attcatGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCcgcgcg < 2:678536/80‑1 (MQ=255)
tcatGTAATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGcgccg > 2:678592/1‑81 (MQ=255)
atgtaatgtgCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGcgccgc > 2:678568/1‑80 (MQ=255)
|
GGAAAGAGATTTGTCCGTTGAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGCCGC > NC_000913/3567442‑3567592
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
