Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,568,321 |
G→A |
G336G (GGC→GGT) |
glgC ← |
glucose‑1‑phosphate adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,568,321 | 0 | G | A | 100.0%
| 120.0
/ NA
| 35 | G336G (GGC→GGT) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/19); total (16/19) |
TGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGATT > NC_000913/3568245‑3568397
|
tGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccaccg > 1:621431/1‑80 (MQ=255)
gTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCgg > 1:621415/1‑80 (MQ=255)
ttGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGa < 2:621349/80‑1 (MQ=255)
ttGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGa < 1:621351/80‑1 (MQ=255)
gCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAAc > 1:621416/1‑81 (MQ=255)
aatgaatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGt > 1:621436/1‑81 (MQ=255)
aatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGt > 2:621421/1‑81 (MQ=255)
atTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGtt < 1:621352/81‑1 (MQ=255)
ttCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTa < 1:621353/81‑1 (MQ=255)
gcgAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGt > 1:621428/1‑81 (MQ=255)
gcgAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGt < 2:621353/81‑1 (MQ=255)
gAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCa < 1:621355/81‑1 (MQ=255)
gAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCa > 2:621413/1‑81 (MQ=255)
gAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCa > 1:621420/1‑81 (MQ=255)
gAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCa > 1:621426/1‑81 (MQ=255)
aaCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCAt < 1:621356/81‑1 (MQ=255)
cgcgcgAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATcc < 1:621357/81‑1 (MQ=255)
gagaACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTgg > 1:621417/1‑81 (MQ=255)
gagaACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTgg > 2:621411/1‑81 (MQ=255)
gaacagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGc > 1:621418/1‑80 (MQ=255)
gaacagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCt < 1:621358/81‑1 (MQ=255)
aacagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTa < 2:621358/81‑1 (MQ=255)
gaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCgg < 2:621359/81‑1 (MQ=255)
cTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATc < 1:621361/81‑1 (MQ=255)
caccaccacCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTg > 1:621442/1‑81 (MQ=255)
accaccaccGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGc < 2:621361/81‑1 (MQ=255)
accGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAAt < 2:621362/81‑1 (MQ=255)
cGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAAttt > 2:621437/1‑81 (MQ=255)
gAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTc > 1:621425/1‑81 (MQ=255)
ggAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTgg < 1:621364/81‑1 (MQ=255)
gagaTCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGc < 2:621364/81‑1 (MQ=255)
agaTCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCg < 2:621365/81‑1 (MQ=255)
acacaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAAt < 1:621367/81‑1 (MQ=255)
cacaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATg < 2:621367/81‑1 (MQ=255)
acaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGa > 2:621429/1‑81 (MQ=255)
aaCCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGAtt > 1:621423/1‑81 (MQ=255)
|
TGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGATT > NC_000913/3568245‑3568397
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
