Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,568,321 |
G→A |
G336G (GGC→GGT) |
glgC ← |
glucose‑1‑phosphate adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,568,321 | 0 | G | A | 97.8%
| 149.7
/ ‑4.2
| 45 | G336G (GGC→GGT) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (22/22); total (22/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 |
ATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGA > NC_000913/3568244‑3568395
|
aTGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccaccg < 1:687566/81‑1 (MQ=255)
tGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCgg < 2:687567/81‑1 (MQ=255)
tGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCgg < 2:687568/81‑1 (MQ=255)
ttGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGa < 2:687569/80‑1 (MQ=255)
tGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGaaa > 2:687627/1‑81 (MQ=255)
cAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAAcc < 2:687570/81‑1 (MQ=255)
cAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAAc > 1:687621/1‑80 (MQ=255)
gaatgaatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAg < 2:687571/81‑1 (MQ=255)
gaatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAg > 2:687641/1‑81 (MQ=255)
aatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAg < 2:687572/80‑1 (MQ=255)
aatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGt > 2:687656/1‑81 (MQ=255)
atTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGt > 1:687619/1‑80 (MQ=255)
cgcgAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAggg < 1:687573/81‑1 (MQ=255)
cgAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTc < 2:687574/81‑1 (MQ=255)
aaCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCAt < 2:687575/81‑1 (MQ=255)
aaCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCAt < 1:687576/81‑1 (MQ=255)
aCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATc < 1:687577/81‑1 (MQ=255)
cgcgcgAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATcc > 1:687646/1‑81 (MQ=255)
gaacagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGc > 2:687635/1‑80 (MQ=255)
gaacagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGc > 1:687634/1‑80 (MQ=255)
aacagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTa < 1:687578/81‑1 (MQ=255)
acagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTAc > 1:687660/1‑81 (MQ=255)
gaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCgg < 2:687579/81‑1 (MQ=255)
gaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCg > 1:687655/1‑80 (MQ=255)
ggACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGc > 1:687676/1‑80 (MQ=255)
ggACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGc > 1:687643/1‑80 (MQ=255)
ggACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGc > 1:687633/1‑80 (MQ=255)
ggACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCg < 1:687580/81‑1 (MQ=255)
ggACTGCACCACCACCGAACCGGAGATCACACAACCACCGAAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCg < 1:687581/81‑1 (MQ=255)
gACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGa > 1:687644/1‑81 (MQ=255)
aCTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGAt < 1:687582/81‑1 (MQ=255)
gCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCt > 1:687628/1‑81 (MQ=255)
accaccaccGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGc < 2:687583/81‑1 (MQ=255)
accaccGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCAc > 2:687647/1‑80 (MQ=255)
ccaccGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGa < 1:687584/81‑1 (MQ=255)
caccGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGaa > 2:687636/1‑81 (MQ=255)
accGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAAt > 2:687650/1‑81 (MQ=255)
cGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAAttt > 2:687683/1‑81 (MQ=255)
aCCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGc > 2:687661/1‑81 (MQ=255)
ggAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTgg > 1:687637/1‑81 (MQ=255)
gagaTCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGc < 1:687585/81‑1 (MQ=255)
agaTCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCg < 1:687586/81‑1 (MQ=255)
acacaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAAt < 2:687588/81‑1 (MQ=255)
acacaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAAt < 2:687587/81‑1 (MQ=255)
cacaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATg < 1:687589/81‑1 (MQ=255)
acaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGa > 1:687645/1‑81 (MQ=255)
|
ATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGA > NC_000913/3568244‑3568395
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
