Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,494,616 |
A→G |
intergenic (‑210/‑7) |
idnD ← / → idnK |
L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,494,616 | 0 | A | G | 100.0%
| 59.0
/ NA
| 18 | intergenic (‑210/‑7) | idnD/idnK | L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (10/8); total (10/8) |
GTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGC > NC_000913/4494545‑4494693
|
gTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGGTTTTAAATg < 2:908437/81‑1 (MQ=255)
gAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGGTTTTAAATGGCg > 1:908461/1‑81 (MQ=255)
gAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTAtt > 2:908463/1‑81 (MQ=255)
tGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGc < 2:908438/80‑1 (MQ=255)
ccgccAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGtt < 1:908439/81‑1 (MQ=255)
cgccAGGCGTAGTATCGCAGCAGGTAAGATGGTTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGttt < 2:908440/81‑1 (MQ=255)
gccAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGttt > 1:908457/1‑80 (MQ=255)
gccAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGttt > 2:908458/1‑80 (MQ=255)
ccAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCa < 2:908441/81‑1 (MQ=255)
gtaTCGCAGCAGGTAAGATGATTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTgg > 1:908477/1‑80 (MQ=255)
gcagcaGGTAAGATGATTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTaaaa > 1:908460/1‑80 (MQ=255)
agcagGTAAGATGATTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACAt > 2:908467/1‑81 (MQ=255)
agcagGTAAGATGATTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACAt < 2:908442/81‑1 (MQ=255)
gTAAGATGATTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAAtt < 1:908443/81‑1 (MQ=255)
gatgatTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTgg > 2:908459/1‑79 (MQ=255)
atgatTCATGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAg < 1:908444/81‑1 (MQ=255)
atgatTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAg > 2:908445/1‑81 (MQ=255)
tgatTCAGGAGGTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAg > 2:908462/1‑80 (MQ=255)
gaggTTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGc > 2:908468/1‑81 (MQ=255)
|
GTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGC > NC_000913/4494545‑4494693
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
