Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,877,592 |
G→A |
V338M (GTG→ATG) |
iap → |
alkaline phosphatase isozyme conversion protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,877,592 | 0 | G | A | 100.0%
| 118.2
/ NA
| 33 | V338M (GTG→ATG) | iap | alkaline phosphatase isozyme conversion protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/11); total (22/11) |
AACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAA > NC_000913/2877518‑2877665
|
aaCATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAgg < 2:522078/81‑1 (MQ=255)
aCATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGa < 2:522079/81‑1 (MQ=255)
cATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAg > 1:522104/1‑81 (MQ=255)
cATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAg > 2:522133/1‑81 (MQ=255)
aTAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCg < 2:522080/80‑1 (MQ=255)
aGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAgg > 2:522110/1‑81 (MQ=255)
ctTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCt > 1:522122/1‑81 (MQ=255)
ccTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCtt < 1:522081/79‑1 (MQ=255)
gaagaaTAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATgg < 1:522082/80‑1 (MQ=255)
gaaTAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGttt > 1:522117/1‑81 (MQ=255)
tAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGa > 1:522107/1‑80 (MQ=255)
gAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGaaaa > 1:522116/1‑81 (MQ=255)
tCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGa < 2:522083/81‑1 (MQ=255)
tCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGa < 1:522084/81‑1 (MQ=255)
tGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTg > 1:522126/1‑81 (MQ=255)
tGCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTg > 2:522121/1‑81 (MQ=255)
gCCGTGACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTgg > 1:522125/1‑81 (MQ=255)
gACGTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGtt > 1:522129/1‑81 (MQ=255)
gTTATGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTa > 2:522130/1‑81 (MQ=255)
aTGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCg > 1:522143/1‑81 (MQ=255)
tGCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCg < 1:522085/80‑1 (MQ=255)
gCGGATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCg > 2:522127/1‑79 (MQ=255)
ggATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCaga < 1:522086/81‑1 (MQ=255)
ggATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCa > 2:522134/1‑79 (MQ=255)
gATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCagag > 1:522153/1‑81 (MQ=255)
gATAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCagag < 1:522087/81‑1 (MQ=255)
aTAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTCGAAAATGGGAGCTGGGAGTTCTACCGCAGAgg > 1:522131/1‑81 (MQ=255)
tAATGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGc > 2:522118/1‑81 (MQ=255)
aaTGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCg > 1:522135/1‑81 (MQ=255)
aTGCTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCgg > 1:522132/1‑81 (MQ=255)
cTACCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCgggg > 1:522137/1‑80 (MQ=255)
aCCTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGaa > 2:522123/1‑81 (MQ=255)
ccTCTGATGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGa < 2:522088/79‑1 (MQ=255)
|
AACATATTGATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAA > NC_000913/2877518‑2877665
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
