Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,962,983 |
(T)5→4 |
coding (174/408 nt) |
ygdB ← |
DUF2509 domain‑containing protein YgdB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,962,979 | 0 | T | . | 100.0%
| 142.7
/ NA
| 34 | coding (178/408 nt) | ygdB | DUF2509 domain‑containing protein YgdB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (20/14); total (20/14) |
CAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAG > NC_000913/2962899‑2963052
|
cAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAt > 2:540376/1‑81 (MQ=255)
cAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAt < 2:540313/81‑1 (MQ=255)
aaCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAttttcc > 2:540377/1‑79 (MQ=255)
caaacaaaCCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCAc > 2:540364/1‑81 (MQ=255)
aaacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAg > 2:540388/1‑81 (MQ=255)
aacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAgc > 1:540367/1‑81 (MQ=255)
aacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAgc < 2:540314/81‑1 (MQ=255)
gggCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGa > 2:540341/1‑81 (MQ=255)
ggCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCg < 2:540315/79‑1 (MQ=255)
cATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTg < 2:540316/81‑1 (MQ=255)
ggTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAAc > 1:540392/1‑80 (MQ=255)
gTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGa < 1:540317/81‑1 (MQ=255)
tttCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGAt > 1:540371/1‑81 (MQ=255)
ttCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATg > 2:540390/1‑81 (MQ=255)
ttCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATg > 1:540386/1‑81 (MQ=255)
cAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGc > 1:540363/1‑81 (MQ=255)
gCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCt < 1:540318/81‑1 (MQ=255)
gCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCt < 1:540319/81‑1 (MQ=255)
cGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTg < 1:540320/81‑1 (MQ=255)
gTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTgg > 2:540393/1‑81 (MQ=255)
gTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTgg > 1:540385/1‑81 (MQ=255)
gTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTgg > 1:540378/1‑81 (MQ=255)
cGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGgcggcg < 2:540321/80‑1 (MQ=255)
cGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGgcggcg > 2:540396/1‑80 (MQ=255)
cGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGgcgc < 2:540322/81‑1 (MQ=255)
aCTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtga > 2:540389/1‑81 (MQ=255)
aaCTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgact < 2:540323/79‑1 (MQ=255)
ctggctgCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACttt < 2:540324/80‑1 (MQ=255)
tggctgCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTc > 1:540399/1‑80 (MQ=255)
ggctgCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCa > 2:540405/1‑81 (MQ=255)
gctgCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGCACGATGGCCTGGCGGCGCAATGACTGACTTTCCAt > 1:540381/1‑81 (MQ=255)
tgCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGc > 1:540403/1‑81 (MQ=255)
gCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCt > 1:540401/1‑81 (MQ=255)
cGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTc < 2:540325/81‑1 (MQ=255)
ccAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAcgc > 2:540395/1‑79 (MQ=255)
cagcagTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCgag < 2:540326/81‑1 (MQ=255)
gcagTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGagag < 1:540327/81‑1 (MQ=255)
|
CAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAG > NC_000913/2962899‑2963052
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
