Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,007,291 |
G→T |
E344* (GAA→TAA) |
ygeW → |
putative carbamoyltransferase YgeW |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,007,291 | 0 | G | T | 100.0%
| 155.5
/ NA
| 43 | E344* (GAA→TAA) | ygeW | putative carbamoyltransferase YgeW |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/29); total (14/29) |
GAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCC > NC_000913/3007213‑3007368
|
gAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTaa < 2:548968/81‑1 (MQ=255)
aCTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGt < 1:548970/81‑1 (MQ=255)
aCTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGt < 2:548969/81‑1 (MQ=255)
tGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGa > 1:549032/1‑81 (MQ=255)
cccGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACt > 1:549035/1‑80 (MQ=255)
cccGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTg < 1:548971/81‑1 (MQ=255)
cccGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTg < 2:548972/81‑1 (MQ=255)
cGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGaa < 1:548973/81‑1 (MQ=255)
gTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAg < 2:548974/81‑1 (MQ=255)
tGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAgg < 2:548975/81‑1 (MQ=255)
gATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGc > 2:549020/1‑81 (MQ=255)
ggCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTa > 2:549051/1‑81 (MQ=255)
ggCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTa > 1:549043/1‑81 (MQ=255)
gAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTc < 1:548976/81‑1 (MQ=255)
aaGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCg < 2:548978/81‑1 (MQ=255)
aaGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCg < 1:548977/81‑1 (MQ=255)
aGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGa < 2:548979/81‑1 (MQ=255)
ccTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGaaa > 2:549048/1‑80 (MQ=255)
tACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATa < 2:548980/80‑1 (MQ=255)
tACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATAc < 1:548981/81‑1 (MQ=255)
cATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATAcc < 2:548983/80‑1 (MQ=255)
cATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCg < 1:548982/81‑1 (MQ=255)
ctgcctgcCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGc < 1:548984/81‑1 (MQ=255)
gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGcta > 1:549049/1‑81 (MQ=255)
tgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGctacc > 1:549021/1‑80 (MQ=255)
aGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACa > 2:549039/1‑80 (MQ=255)
gCTGATATCAGCGGCGTAACCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACaaa < 2:548985/81‑1 (MQ=255)
atatCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAaagaa < 2:548986/80‑1 (MQ=255)
atCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGc > 1:549040/1‑80 (MQ=255)
atCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGc > 2:549036/1‑80 (MQ=255)
tCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGcc < 2:548988/80‑1 (MQ=255)
tCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGcc < 1:548987/80‑1 (MQ=255)
aGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAg < 2:548989/80‑1 (MQ=255)
cggcgTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTg < 2:548990/81‑1 (MQ=255)
cgTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGaa < 2:548991/81‑1 (MQ=255)
cgTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGaa < 2:548992/81‑1 (MQ=255)
gTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAg > 1:549028/1‑81 (MQ=255)
tCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGcc < 2:548993/80‑1 (MQ=255)
cTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCtt < 1:548994/80‑1 (MQ=255)
cTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTta > 1:549046/1‑81 (MQ=255)
aagaagGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATatca > 2:549044/1‑81 (MQ=255)
agaagGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATatcat < 1:548995/81‑1 (MQ=255)
ggTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATcgcc < 1:548996/81‑1 (MQ=255)
|
GAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCC > NC_000913/3007213‑3007368
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
