Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,030,090 |
A→G |
V281A (GTC→GCC) |
ygfT ← |
fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,030,090 | 0 | A | G | 100.0%
| 102.3
/ NA
| 30 | V281A (GTC→GCC) | ygfT | fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/17); total (13/17) |
GCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCT > NC_000913/3030020‑3030167
|
gCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCg < 1:554215/80‑1 (MQ=255)
gACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGt > 2:554250/1‑80 (MQ=255)
cATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGAt < 1:554216/81‑1 (MQ=255)
tCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCt < 1:554217/81‑1 (MQ=255)
tCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCt < 1:554218/81‑1 (MQ=255)
gggACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCttt > 2:554260/1‑81 (MQ=255)
gggACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCttt > 1:554258/1‑81 (MQ=255)
gACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTc > 1:554251/1‑80 (MQ=255)
aCGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCaa < 1:554219/81‑1 (MQ=255)
ccAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAg > 2:554268/1‑80 (MQ=255)
aGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTa > 1:554243/1‑80 (MQ=255)
cccATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACAt < 1:554220/81‑1 (MQ=255)
aTCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATgcg < 1:554221/81‑1 (MQ=255)
cAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCtt > 2:554255/1‑80 (MQ=255)
cAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTc < 2:554222/81‑1 (MQ=255)
gcgcGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTcac < 2:554223/81‑1 (MQ=255)
gcgGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTcaca > 2:554253/1‑80 (MQ=255)
ggTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAg < 2:554224/81‑1 (MQ=255)
gTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGa < 2:554225/81‑1 (MQ=255)
cGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGt > 1:554252/1‑81 (MQ=255)
cGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGt > 2:554264/1‑81 (MQ=255)
tGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCt < 2:554226/81‑1 (MQ=255)
gATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCtg > 2:554256/1‑81 (MQ=255)
aTGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCtgt < 2:554227/81‑1 (MQ=255)
ttCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATAc < 2:554228/81‑1 (MQ=255)
ttACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCg < 2:554229/81‑1 (MQ=255)
aCCGATAGAGGCTGCGCCCGAGTGATCTTTCAAGGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGc < 1:554230/80‑1 (MQ=255)
cGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCaaa < 1:554231/81‑1 (MQ=255)
gATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAAt > 2:554266/1‑81 (MQ=255)
agagGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTc > 2:554254/1‑81 (MQ=255)
gaggCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCt > 2:554257/1‑81 (MQ=255)
|
GCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCT > NC_000913/3030020‑3030167
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
