Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,039,238 |
A→C |
V169G (GTC→GGC) |
xerD ← |
site‑specific recombinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,039,238 | 0 | A | C | 100.0%
| 142.0
/ NA
| 41 | V169G (GTC→GGC) | xerD | site‑specific recombinase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (25/16); total (25/16) |
TGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCA > NC_000913/3039160‑3039315
|
tGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGccc > 1:556461/1‑81 (MQ=255)
ttCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGcccc < 1:556418/80‑1 (MQ=255)
ccAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGt < 1:556419/80‑1 (MQ=255)
aTAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCAc < 2:556420/81‑1 (MQ=255)
tAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACg > 2:556467/1‑81 (MQ=255)
ggTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGc < 1:556421/80‑1 (MQ=255)
gTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGccc > 2:556459/1‑81 (MQ=255)
ccagccagTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGAc > 1:556478/1‑81 (MQ=255)
cagccagTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGAc < 1:556422/80‑1 (MQ=255)
agTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGc > 2:556479/1‑81 (MQ=255)
gTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCt < 2:556423/81‑1 (MQ=255)
aaaCCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGa > 2:556482/1‑81 (MQ=255)
cGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATc < 2:556424/81‑1 (MQ=255)
gCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCa > 1:556462/1‑81 (MQ=255)
ccTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCAc < 1:556425/81‑1 (MQ=255)
ccTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCAc > 1:556460/1‑81 (MQ=255)
ctctTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACt < 2:556426/81‑1 (MQ=255)
ctctTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACt > 2:556474/1‑81 (MQ=255)
tCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCAtt > 2:556485/1‑81 (MQ=255)
cACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCAtt < 1:556427/80‑1 (MQ=255)
cACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTg > 2:556450/1‑81 (MQ=255)
aCCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGt < 2:556428/81‑1 (MQ=255)
tAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAg > 1:556481/1‑81 (MQ=255)
aaCGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGt > 1:556470/1‑81 (MQ=255)
ggCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTcc > 1:556472/1‑80 (MQ=255)
ggCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTcc > 1:556464/1‑80 (MQ=255)
ccAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTTCCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGAc > 1:556465/1‑79 (MQ=255)
cGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGtt > 2:556476/1‑79 (MQ=255)
tctTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCa > 1:556471/1‑78 (MQ=255)
tttGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCagag < 1:556429/79‑1 (MQ=255)
tgttgCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGacac > 1:556473/1‑81 (MQ=255)
gttgCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACg > 2:556469/1‑81 (MQ=255)
gCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAg > 2:556504/1‑81 (MQ=255)
cTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGcc < 1:556430/81‑1 (MQ=255)
tttACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGccc > 2:556483/1‑81 (MQ=255)
tACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCg < 2:556431/80‑1 (MQ=255)
aCCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCgg > 1:556475/1‑80 (MQ=255)
ccAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCgg > 2:556477/1‑79 (MQ=255)
ccAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGt < 1:556432/80‑1 (MQ=255)
cAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAg < 2:556433/81‑1 (MQ=255)
aaTGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGc < 1:556434/81‑1 (MQ=255)
aTGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCTGACCAGTTCAGAGACACGCAGCCCGGTAGCa > 2:556490/1‑81 (MQ=255)
aTGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCa > 1:556501/1‑81 (MQ=255)
|
TGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCA > NC_000913/3039160‑3039315
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
