Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	730	35.1	2107516	91.4%	1926269	80.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,182,453	C→A	intergenic (‑19/‑97)	ygiD ← / → zupT	4,5‑DOPA dioxygenase extradiol/heavy metal divalent cation transporter ZupT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,182,453	0	C	A	100.0%	192.7 / NA	54	intergenic (‑19/‑97)	ygiD/zupT	4,5‑DOPA dioxygenase extradiol/heavy metal divalent cation transporter ZupT
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (32/22); total (32/22)

AAAAACAATGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGA  >  NC_000913/3182376‑3182529

aaaaaaaaTGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCt                                                                           <  2:591021/81‑1 (MQ=255)

   aaCAATGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGt                                                                        >  1:591085/1‑81 (MQ=255)

    aCAATGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGtt                                                                       <  1:591022/81‑1 (MQ=255)

      aaTGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTcg                                                                     >  2:591094/1‑81 (MQ=255)

      aaTGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATACTCACAATAAGCTTGTTcg                                                                     >  2:591075/1‑81 (MQ=255)

        tGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTcggc                                                                   <  1:591023/81‑1 (MQ=255)

        tGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTcggc                                                                   >  1:591099/1‑81 (MQ=255)

          cTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTcggcgg                                                                 <  2:591024/81‑1 (MQ=255)

          cTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTcggcgg                                                                 <  1:591025/81‑1 (MQ=255)

          cTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTcggcg                                                                  >  2:591077/1‑80 (MQ=255)

             gCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAg                                                               <  1:591026/80‑1 (MQ=255)

              cATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGt                                                              >  2:591070/1‑80 (MQ=255)

              cATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTa                                                             <  1:591028/81‑1 (MQ=255)

              cATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTa                                                             <  2:591027/81‑1 (MQ=255)

               aTACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTaa                                                            <  1:591029/81‑1 (MQ=255)

                 aCGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAg                                                           >  2:591071/1‑80 (MQ=255)

                 aCGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAg                                                           >  2:591086/1‑80 (MQ=255)

                        gAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCgg                                                    <  1:591030/80‑1 (MQ=255)

                        gAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGa                                                   >  2:591079/1‑81 (MQ=255)

                             cATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAAc                                               >  1:591074/1‑80 (MQ=255)

                              atgatgATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACaa                                             >  1:591064/1‑81 (MQ=255)

                               tgatgaTATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAat                                            <  2:591031/81‑1 (MQ=255)

                                 atgatATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAatga                                          >  2:591109/1‑81 (MQ=255)

                                   gatATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAatgat                                         >  2:591107/1‑80 (MQ=255)

                                    atatCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAatgatg                                        <  1:591032/80‑1 (MQ=255)

                                     tatCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAatgatgat                                      >  2:591097/1‑81 (MQ=255)

                                         cTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATgatgatgat                                   <  2:591033/80‑1 (MQ=255)

                                         cTTAACTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGatc                                  <  1:591034/81‑1 (MQ=255)

                                            aaCTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGatcat                                >  2:591095/1‑80 (MQ=255)

                                            aaCTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGatcat                                >  2:591106/1‑80 (MQ=255)

                                             aCTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGatcatc                               <  2:591036/80‑1 (MQ=255)

                                             aCTAAAGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGAtcatca                              <  2:591035/81‑1 (MQ=255)

                                                 aaGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGtt                           <  1:591037/80‑1 (MQ=255)

                                                  aGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTAt                         >  1:591091/1‑81 (MQ=255)

                                                  aGGTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTAt                         <  1:591038/81‑1 (MQ=255)

                                                   ggTGTCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTAtt                        >  2:591076/1‑81 (MQ=255)

                                                      gtCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTAtttt                      >  1:591101/1‑80 (MQ=255)

                                                      gtCATTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTAtttt                      >  1:591092/1‑80 (MQ=255)

                                                         aTTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGAc                   >  1:591110/1‑80 (MQ=255)

                                                         aTTTTGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGAc                   <  2:591039/80‑1 (MQ=255)

                                                           tttGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGAt                >  1:591089/1‑81 (MQ=255)

                                                            ttGATATCCTCTCAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATc               >  2:591088/1‑81 (MQ=255)

                                                            ttGATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATc               >  2:591105/1‑81 (MQ=255)

                                                              gATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCt              <  2:591040/80‑1 (MQ=255)

                                                              gATATCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTg             >  2:591087/1‑81 (MQ=255)

                                                               atatCCTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGc            <  1:591041/81‑1 (MQ=255)

                                                                    cTCACAATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTg         >  1:591081/1‑79 (MQ=255)

                                                                      cacaATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAgg     >  1:591119/1‑81 (MQ=255)

                                                                      cacaATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAgg     >  2:591121/1‑81 (MQ=255)

                                                                        caATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGt    >  2:591113/1‑80 (MQ=255)

                                                                        caATAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGt    >  1:591112/1‑80 (MQ=255)

                                                                         aaTAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTg   >  1:591090/1‑80 (MQ=255)

                                                                         aaTAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGa  <  1:591042/81‑1 (MQ=255)

                                                                          aTAAGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTg   >  1:591103/1‑79 (MQ=255)

AAAAACAATGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGA  >  NC_000913/3182376‑3182529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A