Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
182 |
31.9 |
1789978 |
98.5% |
1763128 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,184,175 |
T→C |
T98A (ACC→GCC) |
ribB ← |
3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,184,175 | 0 | T | C | 100.0%
| 145.3
/ NA
| 40 | T98A (ACC→GCC) | ribB | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/24); total (16/24) |
CGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATC > NC_000913/3184097‑3184250
|
cGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCa > 2:545827/1‑81 (MQ=255)
ggCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCAc < 1:545773/81‑1 (MQ=255)
cAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACgg < 2:545774/81‑1 (MQ=255)
cAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACgg > 2:545812/1‑81 (MQ=255)
cAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACgg > 2:545824/1‑81 (MQ=255)
cAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACgg > 2:545840/1‑81 (MQ=255)
aaTCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGt < 1:545775/81‑1 (MQ=255)
aTCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGt > 2:545810/1‑80 (MQ=255)
tCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTaa < 2:545776/81‑1 (MQ=255)
tCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTa > 1:545820/1‑80 (MQ=255)
cGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTaa > 1:545845/1‑80 (MQ=255)
cTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAAc < 1:545777/81‑1 (MQ=255)
cgcgAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAAcc < 1:545778/79‑1 (MQ=255)
gcgAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGt < 1:545780/81‑1 (MQ=255)
gcgAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGt < 2:545779/81‑1 (MQ=255)
gAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGc < 1:545781/81‑1 (MQ=255)
gTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAg > 1:545843/1‑81 (MQ=255)
cGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGgc > 1:545847/1‑81 (MQ=255)
aTACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCt < 1:545783/80‑1 (MQ=255)
aTACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCt < 2:545782/80‑1 (MQ=255)
aCGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGgt > 1:545829/1‑81 (MQ=255)
cGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGgtg < 2:545785/81‑1 (MQ=255)
cGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGgt < 1:545784/80‑1 (MQ=255)
ggTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGgtgt > 2:545832/1‑81 (MQ=255)
aGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTAtt > 1:545831/1‑81 (MQ=255)
cGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTAtttt < 1:545786/81‑1 (MQ=255)
aaCACCGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACcat < 1:545787/81‑1 (MQ=255)
ccGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCAtt < 1:545788/81‑1 (MQ=255)
cGGTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTg < 1:545789/81‑1 (MQ=255)
gTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGc < 2:545790/81‑1 (MQ=255)
gTAGTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGc < 1:545791/81‑1 (MQ=255)
gTCACACCTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAg < 1:545792/80‑1 (MQ=255)
cTTCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAgtt < 2:545793/81‑1 (MQ=255)
ttCAGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAgttg > 2:545835/1‑81 (MQ=255)
aGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAgttgtt < 2:545794/80‑1 (MQ=255)
aGCTGCTTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGttt < 2:545795/81‑1 (MQ=255)
ctgctTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTAc < 2:545796/81‑1 (MQ=255)
ctTCAATGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGc > 2:545842/1‑81 (MQ=255)
aaTGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATc > 2:545836/1‑81 (MQ=255)
aaTGGCCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATc > 1:545833/1‑81 (MQ=255)
|
CGGCAATCGCTGCGCGAACGGTCGTAATACGGTCAGCGGCAGAAACACCGGTAGTCACACCTTCAGCTGCTTCAATGGTCACGGTAAAACCGGTGCCATAGGCGCTGGTGTTATTTTCTACCATCATTGGCAGATCGAGTTGTTTACGGCGATC > NC_000913/3184097‑3184250
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
