Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,184,351 |
T→C |
N39S (AAC→AGC) |
ribB ← |
3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,184,351 | 0 | T | C | 100.0%
| 119.8
/ NA
| 35 | N39S (AAC→AGC) | ribB | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/21); total (14/21) |
CCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTC > NC_000913/3184276‑3184427
|
ccGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTc < 1:591464/80‑1 (MQ=255)
cTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATTATATCACCTTCGCTTTCACgg > 2:591496/1‑81 (MQ=255)
tACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGt > 2:591523/1‑81 (MQ=255)
aCCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTc > 2:591530/1‑81 (MQ=255)
ccGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTc < 1:591465/80‑1 (MQ=255)
ccGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCt < 1:591466/81‑1 (MQ=255)
gTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtc < 2:591467/81‑1 (MQ=255)
gCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtcatc < 1:591469/81‑1 (MQ=255)
gCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtcat < 1:591468/80‑1 (MQ=255)
gCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtcat > 2:591519/1‑80 (MQ=255)
aTGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtcatcatc > 1:591497/1‑80 (MQ=255)
tGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCaa < 1:591470/81‑1 (MQ=255)
tGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCaa < 2:591471/81‑1 (MQ=255)
aGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCAc > 2:591522/1‑80 (MQ=255)
ccATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTa > 1:591521/1‑81 (MQ=255)
cATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTac < 1:591472/81‑1 (MQ=255)
aTCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTaca < 2:591473/81‑1 (MQ=255)
cTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACAcc < 1:591474/78‑1 (MQ=255)
aaCAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGt < 1:591476/79‑1 (MQ=255)
aaCAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTc < 1:591477/80‑1 (MQ=255)
aaCAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTc < 1:591475/80‑1 (MQ=255)
cAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCtt < 2:591478/81‑1 (MQ=255)
tCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAc < 2:591479/81‑1 (MQ=255)
gTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAcgcagc < 1:591480/81‑1 (MQ=255)
gTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAcgcagc < 1:591481/81‑1 (MQ=255)
ttCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAcgcagcg > 1:591527/1‑80 (MQ=255)
tCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAcgcagcgc < 1:591482/80‑1 (MQ=255)
gggAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGt > 2:591544/1‑81 (MQ=255)
aaGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCa > 2:591535/1‑81 (MQ=255)
aGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCAt > 1:591541/1‑81 (MQ=255)
gATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCAtt > 2:591545/1‑81 (MQ=255)
aTCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCAttt > 1:591526/1‑81 (MQ=255)
tCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAac < 2:591483/80‑1 (MQ=255)
aCCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACg < 1:591484/81‑1 (MQ=255)
tCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTc > 2:591529/1‑80 (MQ=255)
|
CCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTC > NC_000913/3184276‑3184427
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
