Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,194,572 |
(T)6→5 |
intergenic (+47/+149) |
yqiK → / ← sibD |
protein YqiK/small RNA SibD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,194,567 | 0 | T | . | 100.0%
| 121.4
/ NA
| 30 | intergenic (+42/+154) | yqiK/sibD | protein YqiK/small RNA SibD |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (14/16); total (14/16) |
TAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAG > NC_000913/3194488‑3194642
|
tAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtt > 2:593453/1‑81 (MQ=255)
ccTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttt < 2:593411/81‑1 (MQ=255)
ccTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttt < 1:593410/81‑1 (MQ=255)
caacTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCt < 1:593412/81‑1 (MQ=255)
acTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGc > 1:593461/1‑81 (MQ=255)
ccGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGt < 1:593413/79‑1 (MQ=255)
cGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGc > 2:593450/1‑80 (MQ=255)
gaagaaAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCt > 2:593458/1‑80 (MQ=255)
gaagaaAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTa < 1:593414/81‑1 (MQ=255)
aagaaAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTaa < 1:593415/81‑1 (MQ=255)
aagaaAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTa > 1:593445/1‑80 (MQ=255)
aaaaaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCTGGGGA‑TTTTTATATCTGCGTTCCGCTaaa > 2:593447/1‑79 (MQ=25)
aaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTg > 1:593460/1‑81 (MQ=255)
aGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGc < 2:593416/81‑1 (MQ=255)
gCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGc > 2:593459/1‑80 (MQ=255)
agagTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCaaa < 2:593417/81‑1 (MQ=255)
gagTAAACCCTCTTTCAGGAATAATCGCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAAt < 1:593418/81‑1 (MQ=18)
gagTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAAt < 2:593419/81‑1 (MQ=255)
agTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg < 2:593420/81‑1 (MQ=255)
aCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg < 1:593422/81‑1 (MQ=255)
aCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg < 2:593421/81‑1 (MQ=255)
cccTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAgg > 1:593452/1‑81 (MQ=255)
ccAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt < 2:593423/78‑1 (MQ=255)
aGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcag > 1:593473/1‑81 (MQ=255)
aataatCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAt < 1:593424/81‑1 (MQ=255)
aataatCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAt > 2:593469/1‑81 (MQ=255)
atCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAtttt < 1:593425/80‑1 (MQ=255)
cccTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCg > 2:593468/1‑81 (MQ=255)
gCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGa < 2:593427/79‑1 (MQ=255)
gCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACg < 2:593426/81‑1 (MQ=255)
aaaaGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCg > 1:593463/1‑81 (MQ=255)
aaaGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgg > 1:593471/1‑81 (MQ=255)
cGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg > 1:593470/1‑81 (MQ=255)
|
TAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAG > NC_000913/3194488‑3194642
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
