Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,194,572 |
(T)6→5 |
intergenic (+47/+149) |
yqiK → / ← sibD |
protein YqiK/small RNA SibD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,194,567 | 0 | T | . | 100.0%
| 149.4
/ NA
| 36 | intergenic (+42/+154) | yqiK/sibD | protein YqiK/small RNA SibD |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (26/10); total (26/10) |
CCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGG > NC_000913/3194491‑3194643
|
ccTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttt < 1:532610/81‑1 (MQ=255)
cTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttta > 1:532648/1‑80 (MQ=255)
caacTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCt < 1:532611/81‑1 (MQ=255)
aacTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTg < 2:532611/81‑1 (MQ=255)
cGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTcc < 1:532613/81‑1 (MQ=255)
cGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTcc > 2:532662/1‑81 (MQ=255)
gTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCg > 1:532649/1‑81 (MQ=255)
gTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCg > 1:532642/1‑81 (MQ=255)
gTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCg > 1:532647/1‑81 (MQ=255)
aaaaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAg > 1:532654/1‑81 (MQ=255)
aaaaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAg > 2:532681/1‑81 (MQ=255)
aGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTg > 2:532640/1‑80 (MQ=255)
aGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTg > 2:532656/1‑80 (MQ=255)
gCAGAGTAAACCCTCTTCCAGGAATAATCTCTGGCCCAAAAAGCCGGGGA‑TCTTTATATCTGCGTTCCGCTAAAAAGTGc > 2:532650/1‑80 (MQ=25)
cAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCa > 1:532650/1‑80 (MQ=255)
agagTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCaaa > 2:532652/1‑81 (MQ=255)
agTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTCTATATCTGCGTTCCGCTAAAAAGTGCAAAt > 2:532644/1‑80 (MQ=21)
aaaCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGAt > 1:532672/1‑80 (MQ=255)
aaCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGAt > 1:532646/1‑79 (MQ=255)
aCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg < 1:532614/81‑1 (MQ=255)
ctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt > 1:532655/1‑81 (MQ=255)
ccAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgtt < 1:532615/79‑1 (MQ=255)
ggAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcagc < 1:532616/81‑1 (MQ=255)
gAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcagca < 2:532616/81‑1 (MQ=255)
aataatCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAt > 1:532665/1‑81 (MQ=255)
atCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAtttt < 1:532618/80‑1 (MQ=255)
atCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTg > 1:532658/1‑81 (MQ=255)
tCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGc < 1:532619/81‑1 (MQ=255)
cTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGt > 1:532668/1‑80 (MQ=255)
ggCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGAc > 1:532664/1‑81 (MQ=255)
cAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCt > 2:532659/1‑81 (MQ=255)
aaaGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgg > 2:532658/1‑81 (MQ=255)
aaaGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgg > 2:532672/1‑81 (MQ=255)
ccGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgggg > 2:532660/1‑79 (MQ=255)
ccGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgggg > 1:532671/1‑79 (MQ=255)
cGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg < 2:532619/81‑1 (MQ=255)
cGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg > 1:532674/1‑81 (MQ=255)
ggggA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAgg > 2:532683/1‑81 (MQ=255)
|
CCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGG > NC_000913/3194491‑3194643
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
