Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,200,368 |
G→A |
Q200* (CAG→TAG) |
ygiF ← |
inorganic triphosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,200,368 | 0 | G | A | 97.1%
| 115.2
/ NA
| 34 | Q200* (CAG→TAG) | ygiF | inorganic triphosphatase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (17/16); total (17/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGC > NC_000913/3200290‑3200443
|
ccAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTacg > 2:595034/1‑81 (MQ=255)
cAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTacg < 1:594966/80‑1 (MQ=255)
ccccTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAg < 1:594967/81‑1 (MQ=255)
ccTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAgat < 2:594968/81‑1 (MQ=255)
cTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAgat > 1:595020/1‑80 (MQ=255)
tGTTCCACATCCTCTTTTTCCGCCACATGCAACATGGTGGTCGGTTTGATTTCACTCGCCGGATTTCCCTACCCCAgttga > 2:1035738/1‑81 (MQ=255)
acaTCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCa < 1:594969/81‑1 (MQ=255)
ttttGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGcgccgc > 2:595047/1‑81 (MQ=255)
cGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCt < 1:594970/81‑1 (MQ=255)
cAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCt > 2:595026/1‑79 (MQ=255)
aaCATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTGAg > 2:595061/1‑81 (MQ=255)
aaCATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAg > 1:595050/1‑81 (MQ=255)
aaCATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAg < 1:594971/81‑1 (MQ=255)
aCATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAgg > 1:595055/1‑81 (MQ=255)
aTGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCt < 1:594972/81‑1 (MQ=255)
cAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGcc < 2:594973/81‑1 (MQ=255)
aaaaTGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGccc > 2:595054/1‑81 (MQ=255)
aaaaTGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGccc > 2:595039/1‑81 (MQ=255)
tggtggTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGc < 2:594975/81‑1 (MQ=255)
tggtggTCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGc < 1:594974/81‑1 (MQ=255)
ggtggtCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCg > 2:595042/1‑81 (MQ=255)
gtggtCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGt < 2:594977/81‑1 (MQ=255)
gtggtCGGTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGt < 1:594976/81‑1 (MQ=255)
gTTTGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCgg < 2:594978/81‑1 (MQ=255)
tGATTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGtt < 2:594979/80‑1 (MQ=255)
aTTTCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGc > 2:595040/1‑81 (MQ=255)
tttCACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCg < 1:594980/81‑1 (MQ=255)
cACGCGCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATa > 2:595051/1‑81 (MQ=255)
cgcgCCGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATAc > 1:595046/1‑80 (MQ=255)
cGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTg > 2:595053/1‑81 (MQ=255)
cGGATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTg < 1:594981/81‑1 (MQ=255)
ggATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTgg > 1:595057/1‑81 (MQ=255)
gATTGCCCTGCGCCAGATGATAACCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGt < 1:594982/81‑1 (MQ=255)
gATTGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGt > 1:595060/1‑81 (MQ=255)
ttGCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTc < 2:594983/81‑1 (MQ=255)
gCCCTACGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGc > 2:595067/1‑81 (MQ=255)
|
CCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGTCGGTTTGATTTCACGCGCCGGATTGCCCTGCGCCAGATGATAGCCACGCGCCGCTTTGCTCAGGCTGCCCTGGCGTAATCCGGTTTGCGATACCAGTTGGTTCGC > NC_000913/3200290‑3200443
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
